二苯乙烯化合物的提取工艺优化及其酪氨酸酶抑制活性

该研究探讨了桑根来源的三种二苯乙烯化合物(氧化白藜芦醇,白藜芦醇和白藜芦醇苷)的富集提取工艺,并对提取物的抗氧化活性和酪氨酸酶抑制作用进行了研究。结果表明,通过单因素试验和响应面试验确定富集二苯乙烯化合物的最佳条件为:提取时间1h,料液比1:20,乙醇浓度90%,在此条件下,桑根中氧化白藜芦醇,白藜芦醇和白藜芦醇苷的含量分别为2923.59μg/g,32.45μg/g和3.15μg/g,富含三种二苯乙烯化合物提取物的得率为10.23%。并且,提取物表现出极强的DPPH自由基清除能力(IC_(50)=0.063 mg/mL)和ABTS自由基清除能力(IC_(50)=0.008 mg/mL)。此外,提取物比熊果苷表现出更强的酪氨酸酶抑制活性,其酪氨酸酶抑制率IC_(50)(2.514μg/m L)仅约为熊果苷(IC_(50)=15.551μg/m L)的16%。综上,高活性的二苯乙烯化合物提取物具有天然抗氧化剂和美白剂的潜力,在食品、美容与医药领域有很大的应用价值。桑根可作为二苯乙烯化合物的重要天然来源,是值得被开发利用的抗氧化、美白的原料。

菟丝草植物的研究进展

目的总结分析近10年对菟丝草植物的相关研究进展。方法查阅国内外有关菟丝草和菟丝子的主要化学成分、生物活性、质量标准控制及其生药鉴别等文献,对其进行总结和归纳分析。结果菟丝草植物具有寄生性,其成分及生物活性受寄主影响。在多种活性成分中,黄酮类含量居多。菟丝草植物有抗氧化等多种功效。结论菟丝草植物中菟丝子的研究较广泛,对其全草研究多集中在其质量标准方面。

Presence of Obstetrical Conditions among Women with Pelvic Inflammatory Diseases: Experience of 50 Cases in Bangladesh

Background: Pelvic Inflammatory Diseases can be associated with the different obstetrical condition. Objectives: The purpose of the present study was to see the Presence of Obstetrical Conditions among Women with pelvic inflammatory diseases (PID). Methodology: This cross-sectional of study was conducted in the Department of Obstetrics and Gynaecology at Dhaka Medical College, Dhaka from January 2007 to June 2007 for a period of six (6) months. Women with the age group of 15 - 45 years presented with lower abdominal pain, tenderness, per vaginal discharge and cervical motion tenderness were included in this study. A pre-designed data collection sheet was used to record the history of different obstetrical condition. Result: A total number of 50 women presented with PID were recruited for this study of which majority was in para 2 to 5 which was 30 (60.0%) cases. Majority of the patients had given the history of home delivery (74.0%) which was performed by untrained birth attendance (60.0%). The history of induced abortion was given by 24.0% cases. History of more than 2 times menstrual regulation (MR) and 2 to 5 times were performed in 20 (40.0%) cases in each. Among 50 cases majority used intrauterine device (IUCD) which were 20 (40.0%) cases. Conclusion: In conclusion majority of the women presented with PID have given the history of para 2 to 5, home delivery with untrained birth attendance, induction abortion and history of more than two times MR.

Diagnostic Validity of Cica Beta Test 1 for the Detection of Extended Spectrum Beta-Lactamase (ESBL) Producing Gram Negative Bacteria by Comparing with Phenotypic Method

Background: Detection of extended spectrum beta lactamase producing bacteria is an important issue in the clinical settings. Objective: The purpose of the present study was to validate the Cica Beta Test 1 for detection of extended spectrum beta-lactamase (ESBL) producing bacteria. Method: This analytical type of cross-sectional study was carried out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from January 2006 to December 2006 for a period of one (01) year. All the patients presented with the clinical features of urinary tract infection and surgical as well as burn wound infection at any age with both sexes were selected as study population. All bacteria were isolated and identified by their colony morphology, staining characters, pigment production, motility and other relevant biochemical tests. Phenotypic confirmation of ESBLs producing isolates were done by inhibitor potentiated disc diffusion test according to CLSI recommendation. The Cica Beta Test 1 was performed according to the manufacturer’s instructions. Result: A total number of 288 Gram negative bacteria were isolated. Among these isolates Cica Beta test 1 was positive in 97 strains and phenotypic confirmatory test was positive in 89 strains. The test sensitivity of Cica Beta Test 1 was 100% (95% CI 95.9% to 100.0%). Specificity of the test was 96.0% (95% CI 92.2% to 98.2%). The positive predictive value (PPV) and negative predictive value (NPV) were 92.7% (95% CI 84.5% to 95.7%) and 100.0% (95% CI 98.0% to 100.0%) respectively. The accuracy of the test was 97.2% (95% CI 95.1% to 99.1%). Area under ROC curve = 0.980 (95% CI 0.964 to 0.996);p value 0.0001. Conclusion: In conclusion, Cica Beta Test 1 is very high sensitivity and specificity for the detection of ESBL from Gram negative bacteria.

Surgical Outcomes of Spinal Tumour: Experiences of 48 Cases at Referral Neurosurgery Hospital in Bangladesh

Background: Spinal tumour has a great morbidity. Objective: The purpose of the present study was to see the outcome of the spinal tumour surgery. Methodology: Patients with spinal tumor have undergone surgery in neurospine unit of National Institute of Neurosciences and Hospital, Dhaka, Bangladesh from May 2013 to March 2015 for a period of 23(twenty three) months. Plain X-ray and MRI were done in all cases. All patients have undergone surgery through posterior midline approach. They were evaluated preoperatively and at discharge usually on 10th postoperative day after stitch removal and advised to attend in follow up clinic after 2 months of surgery. Result: 50 - 59 years age group was observed as most vulnerable for tumor occurrence (23 cases, 47.9%). The male female ratio was 1:1.3. The highest number (28 cases, 58.4%) of tumor was observed in thoracic region but the highest variety was schwannoma (33 cases, 68.7%). Initial presentation of patients was pain (32 cases, 66.7%), motor disturbances (9 cases 18.7%), sensory disturbances (5 cases 10.4%) and sphincter disturbances (2 cases 4.2%). Symptomatic improvement was in 44 (91.7%) patients whereas improvement in Frankel Scale was observed in 10 (20.8%) patients during follow up. 4 (8.4%) patients deteriorated and there was no death in this series. Conclusion: In conclusion, majority of the spinal tumour patients are presented with schwannoma with good symptomatic improvement.

库鲁木提草多糖成分及其抗病毒活性研究

采用热水提取、乙醇沉淀等方法制备新疆药用植物库鲁木提草的多糖,利用紫外和红外仪器、1-苯基-3-甲基-5-吡唑啉酮柱前衍生法等对其组成进行了分析.通过半叶枯斑法分析了其抗烟草花叶病毒(TMV)活性.库鲁木提草90%醇沉除蛋白部位多糖(HNP90)的糖含量和蛋白质含量分别为63.6%和2.98%.红外光谱结果显示,HNP90呈现明显的多糖特征吸收峰.单糖组成研究结果表明库鲁木提草多糖样品HNP90主要含有6种单糖.抗TMV结果显示HNP90的浓度为500 mg/mL时,其对感染TMV的保护、治疗和钝化活性,与对照药利巴韦林接近,具有显著的抗病毒活性.通过对新疆特色药用植物库鲁木提草多糖成分及其抗病毒活性进行研究,为库鲁木提草药用植物中多糖的开发和应用提供了参考.

黄花软紫草的多糖成分及其抗病毒活性研究

采用水提醇沉法,提取黄花软紫草水溶性多糖,利用紫外-可见分光光度法、傅立叶变换红外光谱法和1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱(PMP-HPLC)法对其化学成分进行了分析.应用半叶枯斑法分析了黄花软紫草水溶性多糖抗烟草花叶病毒(TMV)活性.通过半叶枯斑法分析了其抗烟草花叶病毒(TMV)活性.结果表明,黄花软紫草水溶性多糖AG90的糖含量和蛋白质含量分别为81.0%和8.1%.红外光谱结果呈现明显的多糖特征吸收峰,结构为吡喃环.AG90是由6种单糖构成的水溶性杂多糖,组成单糖如下:甘露糖、鼠李糖、葡萄糖、半乳糖、木糖和阿拉伯糖.抗TMV结果显示,当AG90的浓度为500 mg/mL时,其抗TMV的保护、治疗和钝化活性均优于阳性对照药利巴韦林.

UPLC法测定库鲁木提草成分及其指纹图谱研究

运用超高效液相色谱(ultrahigh performance liquid chromatography,缩写为UPLC)技术建立快速、高效、全面评价中药库鲁木提草质量的指纹图谱方法.流动相用乙腈-0.3%磷酸水溶液对样品进行梯度洗脱,流速设置为0.32 mL/min,检测波长为220 nm,柱温为30℃,进样量为2μL,采用Waters公司Acquity UPLC BEH C18(1.7μm, 2.1 mm×100 mm)色谱柱,共计分析了10个不同地区中库鲁木提草的UPLC色谱图,初步建立了库鲁木提草UPLC指纹图谱,运用中药色谱指纹图谱相似度评价系统进行分析,共确定了12个共有峰,通过与标准物质保留时间的对比进行分析,初步确认了其中的3个化合物.结果表明本方法为中药库鲁木提草提供了操作简便、快速、重复性好的质量评价方法,可为中药库鲁木提草的质量控制提供一定的参考.