Dear editor,The term“syringocele”originated from Greek words“syringo”meaning tube and“cele”meaning swelling and was first coined by Maizels et al.[1]in 1983 to represent a spectrum of dilatation of the normal Co...Dear editor,The term“syringocele”originated from Greek words“syringo”meaning tube and“cele”meaning swelling and was first coined by Maizels et al.[1]in 1983 to represent a spectrum of dilatation of the normal Cowper’s gland duct.They classified syringocele into four morphological types:Simple,perforate,imperforate,and ruptured.Melquist et al.[2]simplified the classification by dividing syringocele into two groups:Closed and open syringocele.Usually diagnosed in the pediatric population,only 10 cases of adult syringocele were reported in literature till 2007[3].Endoscopic management is preferred in most symptomatic adolescent patients and various energy sources employed for endoscopic marsupialization include cold knife urethrotomy,electrocautery,or holmium laser[4,5].Use of holmium laser is reported only in three cases with maximum follow-up of up to 1 year[6e8].We herein report three additional cases of adolescent closed syringocele,provide detailed technique of laser marsupialization,and report its long-term postoperative outcome.The study was approved by the University of Miami Ethics Committee(study ID:20180511).展开更多
We present first results from a hybrid coplanar waveguide microfluidic tank circuit for monitoring lipid bilayer formation and fluctuations of integrated proteins. The coplanar waveguide is a radio frequency resonator...We present first results from a hybrid coplanar waveguide microfluidic tank circuit for monitoring lipid bilayer formation and fluctuations of integrated proteins. The coplanar waveguide is a radio frequency resonator operating at ~250 MHz. Changes within the integrated microfluidic chamber, such as vesicle bursting and subsequent nanopore formation alter the reflected signal, and can be detected with nanosecond resolution. We show experimental evidence of such alterations when the microfluidic channel is filled with giant unilamellar vesicles (GUVs). Subsequent settling and bursting of the GUVs form planar lipid bilayers, yielding a detectable change in the resonant frequency of the device. Results from finite element simulations of our device correlate well with our experimental evidence. These simulations also indicate that nanopore formation within the bilayer is easily detectable. The simulated structure allows for incorporation of microfluidics as well as simultaneous RF and DC recordings. The technique holds promise for high throughput drug screening applications and could also be used as an in-plane probe for various other applications. It opens up possibilities of exploring ion channels and other nano scale pores in a whole new frequency band allowing for operating at bandwidths well above the traditional DC methods.展开更多
文摘Dear editor,The term“syringocele”originated from Greek words“syringo”meaning tube and“cele”meaning swelling and was first coined by Maizels et al.[1]in 1983 to represent a spectrum of dilatation of the normal Cowper’s gland duct.They classified syringocele into four morphological types:Simple,perforate,imperforate,and ruptured.Melquist et al.[2]simplified the classification by dividing syringocele into two groups:Closed and open syringocele.Usually diagnosed in the pediatric population,only 10 cases of adult syringocele were reported in literature till 2007[3].Endoscopic management is preferred in most symptomatic adolescent patients and various energy sources employed for endoscopic marsupialization include cold knife urethrotomy,electrocautery,or holmium laser[4,5].Use of holmium laser is reported only in three cases with maximum follow-up of up to 1 year[6e8].We herein report three additional cases of adolescent closed syringocele,provide detailed technique of laser marsupialization,and report its long-term postoperative outcome.The study was approved by the University of Miami Ethics Committee(study ID:20180511).
文摘We present first results from a hybrid coplanar waveguide microfluidic tank circuit for monitoring lipid bilayer formation and fluctuations of integrated proteins. The coplanar waveguide is a radio frequency resonator operating at ~250 MHz. Changes within the integrated microfluidic chamber, such as vesicle bursting and subsequent nanopore formation alter the reflected signal, and can be detected with nanosecond resolution. We show experimental evidence of such alterations when the microfluidic channel is filled with giant unilamellar vesicles (GUVs). Subsequent settling and bursting of the GUVs form planar lipid bilayers, yielding a detectable change in the resonant frequency of the device. Results from finite element simulations of our device correlate well with our experimental evidence. These simulations also indicate that nanopore formation within the bilayer is easily detectable. The simulated structure allows for incorporation of microfluidics as well as simultaneous RF and DC recordings. The technique holds promise for high throughput drug screening applications and could also be used as an in-plane probe for various other applications. It opens up possibilities of exploring ion channels and other nano scale pores in a whole new frequency band allowing for operating at bandwidths well above the traditional DC methods.
