真武汤联合新辅助化疗对卵巢癌病灶内癌细胞恶性特征的影响

目的:探讨真武汤联合新辅助化疗对卵巢癌病灶内癌细胞恶性特征的影响。方法:选择在本院接受手术治疗的卵巢癌患者80例,回顾治疗方案并分为接受术前新辅助化疗的对照组42例、接受术前真武汤联合新辅助化疗的真武汤组38例。留取术中卵巢癌组织并采用荧光定量PCR法检测其中增殖基因、侵袭基因、自噬基因的表达量。结果:真武汤组患者卵巢病灶组织中增殖基因AKT、LSD1、SIRT1、NOB1mRNA的表达量低于对照组;侵袭基因DACT1 mRNA的表达量高于对照组,MTA1、GRP78mRNA的表达量低于对照组;自噬基因Beclin1 mRNA的表达量高于对照组,LC3-Ⅱ、Atg5mRNA的表达量低于对照组。结论:卵巢癌患者术前接受真武汤联合新辅助化疗,可有效抑制肿瘤细胞的增殖及侵袭活性并调节其自噬功能。

Effect of zhenwu decoction combined with neoadjuvant chemotherapy on malignant characteristics of cancer cells in ovarian cancer

Objective:To explore the effect of zhenwu decoction combined with neoadjuvant chemotherapy on malignant characteristics of cancer cells in ovarian cancer.Methods:A total of 80 patients with ovarian cancer who underwent surgical treatment in this hospital between March 2015 and January 2017 were divided into the control group (n=42) who received preoperative neoadjuvant chemotherapy and the zhenwu decoction group (n=38) who received preoperative zhenwu decoction combined with neoadjuvant chemotherapy. Intraoperative ovarian cancer tissue was kept to detect the expression of proliferation genes, invasion genes and autophagy genes through fluorescence quantitative PCR method.Results:Proliferation genes AKT, LSD1, SIRT1 and NOB1 mRNA expression in ovarian lesion tissue of zhenwu decoction group were lower than those of control group;invasion gene DACT1 mRNA expression was higher than that of control group whereas MTA1 and GRP78 mRNA expression were lower than those of control group;autophagy gene Beclin1 mRNA expression was higher than that of control group whereas LC3-Ⅱ and Atg5 mRNA expression were lower than those of control group.Conclusion:Preoperative zhenwu decoction combined with neoadjuvant chemotherapy can effectively inhibit the proliferation and invasion activity of tumor cells and regulate their autophagy function in patients with ovarian cancer.