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5-氮杂-2'-脱氧胞苷体外对黑素瘤细胞系肿瘤相关抗原和免疫分子表达的影响
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作者 柴丽娜 王捷熙 +9 位作者 李伟静 刘敏霞 王璇琳 王艳 贺敏 杨超 于群 韩颖 任素萍 adam i.riker 《军事医学》 CAS CSCD 北大核心 2013年第10期740-744,共5页
目的探讨5-氮杂-2'-脱氧胞苷(5-aza-2'-deoxycytidine,5-aza-CdR)对4种体外培养建系的黑素瘤细胞肿瘤相关抗原和免疫分子表达水平的影响。方法将手术切除或细针穿刺活组织检查获得的黑素瘤组织体外培养建系;应用流式细胞术检测5... 目的探讨5-氮杂-2'-脱氧胞苷(5-aza-2'-deoxycytidine,5-aza-CdR)对4种体外培养建系的黑素瘤细胞肿瘤相关抗原和免疫分子表达水平的影响。方法将手术切除或细针穿刺活组织检查获得的黑素瘤组织体外培养建系;应用流式细胞术检测5-aza-CdR用药后黑素瘤细胞表面人类白细胞抗原(human leukocyte antigen,HLA)-Ⅰ类、-Ⅱ类、-A2以及细胞间黏附分子1(intercellular adhesion molecule 1,ICAM-1)表达的变化;采用实时定量RT-PCR检测用药后一系列重要的肿瘤相关抗原表达的变化。结果 5-aza-CdR用药后,4种黑素瘤细胞系HLA-Ⅰ类、-Ⅱ类和-A2的表达与对照组相比无明显变化;TC12A和TC13细胞ICAM-1表达明显升高;TC12A、TC13和TC69B细胞肿瘤相关抗原黑素瘤抗原基因(MAGE)-4和NY-ESO-1的表达水平明显升高。结论 5-aza-CdR不影响黑素瘤细胞HLA的表达水平,对ICAM-1和某些黑素瘤相关肿瘤抗原的表达有明显上调作用,提示化疗药物5-aza-CdR不会阻碍抗肿瘤免疫中黑素瘤细胞的抗原呈递能力,为化疗联合免疫治疗肿瘤提供了理论前提。 展开更多
关键词 黑色素瘤 5-氮杂-2’-脱氧胞苷 HLA抗原 ICAM-1 肿瘤相关抗原
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Cyclin G2,a novel target of sulindac to inhibit cell cycle progression in colorectal cancer 被引量:2
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作者 Hongyou Zhao Bin Yi +4 位作者 Zhipin Liang Ches’Nique Phillips Hui-Yi Lin adam i.riker Yaguang Xi 《Genes & Diseases》 SCIE 2021年第3期320-330,共11页
Sulindac has shown significant clinical benefit in preventing colorectal cancer pro-gression,but its mechanism of action has not been fully elucidated.We have found that sulin-dac sulfide(SS)is able to inhibit cell cy... Sulindac has shown significant clinical benefit in preventing colorectal cancer pro-gression,but its mechanism of action has not been fully elucidated.We have found that sulin-dac sulfide(SS)is able to inhibit cell cycle progression in human colorectal cancer cells,particularly through G1 arrest.To understand the underlying mechanisms of sulindac inhibitory activity,we have demonstrated that Cyclin G2 up-regulation upon SS treatment can substan-tially delay cell cycle progression by enhancing the transcriptional activity of FOXO3a in human colorectal tumor cells.MiR-182,an oncogenic microRNA known to inhibit FOXO3a gene expres-sion,is also involved in the suppressive effect of SS on cell cycle progression.This process be-gins with the down-regulation of miR-182,followed by the enhancement of FOXO3a transcriptional activity and the up-regulation of Cyclin G2.To further determine the clinical utility of this axis,we analyzed the expression of miR-182/FOXO3a/Cyclin G2 in human colo-rectal tumor samples.Our results show not only that there are significant dfferences in miR-182/FOXO3a/Cyclin G2 between tumors and normal tissues,but also that the synergetic effect of miR-182 and FOXO3a is associated with predicting tumor progression.Our study dem-onstrates a novel mechanistic axis consisting of miR-182/FOXO3a/Cyclin G2 that mediates su-lindac inhibition of cell cycle progression. 展开更多
关键词 Cell cycle Colorectal cancer Gene regulation miRNA SULINDAC
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