Objective:To determine the phytochemicals,total saponin content and types of saponin present in leaf extracts of Cassytha filiformis.Methods:The leaves were extracted with n-hexane and methanol.The methanol extract wa...Objective:To determine the phytochemicals,total saponin content and types of saponin present in leaf extracts of Cassytha filiformis.Methods:The leaves were extracted with n-hexane and methanol.The methanol extract was fractionated.The total saponin content of the butanol fraction was determined by colorimetry via a UV spectrophotometer and ginsenoside was used as the standard.Measurements were carried out at 550 nm.The butanol fraction was subjected to gas chromatography-mass spectrometer analysis.Results:All screened phytochemicals were absent in the n-hexane extract while saponins,steroids,tannins and glycosides were present in the methanol extract.Flavonoids and alkaloids were absent.The total saponin content of the methanol extract is 73.47μg ginsenoside Rb1 equivalent/g extract.The chromatography-mass spectrometer analysis gave eicosanoic acid,methyl ester as the most abundant compound and the steroidal saponin,cholestan-7-one and cyclic 1,2-ethanedienyl acetal as the most abundant saponin in the butanol fraction.Conclusions:The leaves of Cassytha filiformis are rich in steroidal saponins.展开更多
文摘Objective:To determine the phytochemicals,total saponin content and types of saponin present in leaf extracts of Cassytha filiformis.Methods:The leaves were extracted with n-hexane and methanol.The methanol extract was fractionated.The total saponin content of the butanol fraction was determined by colorimetry via a UV spectrophotometer and ginsenoside was used as the standard.Measurements were carried out at 550 nm.The butanol fraction was subjected to gas chromatography-mass spectrometer analysis.Results:All screened phytochemicals were absent in the n-hexane extract while saponins,steroids,tannins and glycosides were present in the methanol extract.Flavonoids and alkaloids were absent.The total saponin content of the methanol extract is 73.47μg ginsenoside Rb1 equivalent/g extract.The chromatography-mass spectrometer analysis gave eicosanoic acid,methyl ester as the most abundant compound and the steroidal saponin,cholestan-7-one and cyclic 1,2-ethanedienyl acetal as the most abundant saponin in the butanol fraction.Conclusions:The leaves of Cassytha filiformis are rich in steroidal saponins.
