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Colorimetric Determination of Monosodium Glutamate in Food Samples Using Colorimetric Determination of Monosodium Glutamate in Food Samples Using L-glutamate Oxidase-glutamate Oxidase 被引量:1
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作者 afraa alnokkari Mounir Ataie Zaid Alasaf 《应用与环境生物学报》 CAS CSCD 北大核心 2013年第6期1069-1072,共4页
A rapid, sensitive and simple chromogenic method was developed for quantitative determination of monosodium glutamate(MSG) in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H 2 ... A rapid, sensitive and simple chromogenic method was developed for quantitative determination of monosodium glutamate(MSG) in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H 2 O 2 from a glutamate sample as a result of enzymatic action was measured using 4-aminoantipyrine and phenol as a chromogenic reagent at 502 nm. Glutamate calibration curve was linear up to 125 mmol L-1 with a detection limit of 2 mmol L-1. The results of analyzing L-glutamate in various food samples using the approach were compared to those of a standard procedure employing HPLC method. MSG concentration in different samples ranged from 0.93 to 4.9 g/kg. This method is relatively sensitive and specifi c, without the need of pre-treatment for sample. Fig 5, Tab 1, Ref 展开更多
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