Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leish...Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leishmania seropositive(n=124).patients seropositive but without clinical presentation(n=82) and healthy controls(n=63) were selected to conduct this cross-sectional study.Polymorphism at +2767 position of IL-8 was investigated using PCR-RPLP techniques.Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.Results:We observed higher significant frequencies +2767 A/A and A/T genotypes in groups I compared to group 2 and healthy controls(P=0.001).Also,patients in Group 1 carriyng A/A genotype showed higher liter of antileshmania antibody than patients with A/T and T/T genotypes(P=0.05).The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance(ANOVA),as well as x^2tests.Conclusions:Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress.展开更多
Objective:To study Leishmania infection in cats and its potential role in triuismission of the disease to human by parasitological,serological and molecular methods in Ahar District,East Azerbaijan Province.Methods:In...Objective:To study Leishmania infection in cats and its potential role in triuismission of the disease to human by parasitological,serological and molecular methods in Ahar District,East Azerbaijan Province.Methods:In this study,65 cats from different parts of Ahar Province were trapped.The cats were anesthetized with chloroform and blood samples were taken from jugular vein and tested by direct agglutination lest.Spleen and liver smear samples were prepared in order to microscopically examine these organs,and also cultured in Novy-MucNeal-Nicolle and Roswell Park Memorial Institute 1 641) media.Finally,spleen tissue DNA was extracted to perform polymerase chain reaction analysis.Results:In direct agglutination test,4(6%) cats had a positive titer,while 14(22%) cats had a titer of 1:80 which was suspected for an infection and 47(729c) cats were negative.Culture results were negative and ill polymerase chain reaction no amplification was observed.Conclusions:Wc found no case of feline visceral leishmaniasis.It needs more extensive studies by using a larger number of cats to firmly establish leishmaniasis in this area.展开更多
基金supported by a grant from the Tabriz University of Medical Sciences(No.91-20)
文摘Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leishmania seropositive(n=124).patients seropositive but without clinical presentation(n=82) and healthy controls(n=63) were selected to conduct this cross-sectional study.Polymorphism at +2767 position of IL-8 was investigated using PCR-RPLP techniques.Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.Results:We observed higher significant frequencies +2767 A/A and A/T genotypes in groups I compared to group 2 and healthy controls(P=0.001).Also,patients in Group 1 carriyng A/A genotype showed higher liter of antileshmania antibody than patients with A/T and T/T genotypes(P=0.05).The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance(ANOVA),as well as x^2tests.Conclusions:Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress.
基金Supported by Infectious Disease and Tropical Research Center of Tabriz University of Medical Sciences(Project No.92-02)
文摘Objective:To study Leishmania infection in cats and its potential role in triuismission of the disease to human by parasitological,serological and molecular methods in Ahar District,East Azerbaijan Province.Methods:In this study,65 cats from different parts of Ahar Province were trapped.The cats were anesthetized with chloroform and blood samples were taken from jugular vein and tested by direct agglutination lest.Spleen and liver smear samples were prepared in order to microscopically examine these organs,and also cultured in Novy-MucNeal-Nicolle and Roswell Park Memorial Institute 1 641) media.Finally,spleen tissue DNA was extracted to perform polymerase chain reaction analysis.Results:In direct agglutination test,4(6%) cats had a positive titer,while 14(22%) cats had a titer of 1:80 which was suspected for an infection and 47(729c) cats were negative.Culture results were negative and ill polymerase chain reaction no amplification was observed.Conclusions:Wc found no case of feline visceral leishmaniasis.It needs more extensive studies by using a larger number of cats to firmly establish leishmaniasis in this area.
