Leptadenia pyrotechnica is an important multipurpose endangered plant in the Kingdom of Bahrain with restricted distribution. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different...Leptadenia pyrotechnica is an important multipurpose endangered plant in the Kingdom of Bahrain with restricted distribution. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of indole acetic acid (IAA) and 6-benzylaminopurine (BAP). Initially, 80% and 60% explants responded in direct shoot and callus initiation response respectively in presence of 8.88 μM BAP with 5.71 μM IAA in modified MS media after two weeks of culture. The highest frequency of plant regeneration was observed in presence of 8.88 μM BAP with 1.14 μM IAA following organogenic pathway of differentiation. Hundred percent callus proliferation was observed while initial callus developed in presence of 4.44 μM BAP with 2.85 μM IAA and was transferred in media containing 4.44 μM, 6.66 μM BAP with 2.85 μM IAA and 13.32 μM BAP with 5.71 μM IAA. The callus derived plants were regenerated following the pathway of indirect somatic embryogenesis. The induction of somatic embryogenesis and plant regeneration from callus was also observed in modified MS media supplemented with 4.44 μM BAP and 2.85 μM IAA. The plant regeneration protocol we developed for Leptadenia pyrotechnica will be very beneficial for biodiversity conservation and environment protection of Bahrain. Moreover, the present paper reports for the first time specifically the somatic embryogenesis in this multipurpose desert plant Leptadenia pyrotechnica.展开更多
Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conserva...Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conservation of this plant applying tissue culture method was used in the present study. In vitro plant regeneration has been established via shoot organogenesis using nodal meristems. The effect of various plant growth regulators was evaluated to regenerate the whole plants by in vitro micropropagation in modified MS media supplemented with different plant growth regulators (PGRs). In vitro regeneration of plants was involved in three steps by shoot initiation, multiplication and rooting of microshoots. Initially 100% explants of Heliotropium kotschyi responded to initiate shoot in MS medium supplemented with 8.88 μM BAP with 5.71 μM IAA after 4 weeks of culture. Multiplication of shoots and ultimate plant regeneration capacity of Heliotropium kotschyi were compared and the highest performance of 578 shoots/explant was observed in presence of 8.88 μM BAP and 5.71 μM IAA after second transfer. Rooted plantlets were transferred to soil pots and 60% plants were survived after one and a half months. The highly efficient plant regeneration protocol of the desert plant is of great value in rapid plant propagation program for the purpose of conservation of biodiversity.展开更多
Partial Rootzone Drying (PRD) is an irrigation technique which offers a means of modifying the growth and development of crops through relatively simple changes to the method of water delivery. The technique causes th...Partial Rootzone Drying (PRD) is an irrigation technique which offers a means of modifying the growth and development of crops through relatively simple changes to the method of water delivery. The technique causes the stimulation of physiological responses which are normally associated with water stress and this results in a significant reduction in water use through the production of chemical signals in drying roots. Partial drying of one half of the roots of plants grown with two root systems is rapidly translated into a reduction in transpiration and assimilation of all the crop leaves. The aim of this research is to examine and simulate the novel irrigation method (PRD), which would stimulate the endogenous stress response mechanisms of Conocarpus erectus trees in the pilot area of the Arabian Gulf University in Bahrain and wheat and maize crops in the Mashtul Pilot Area (MPA), Egypt using Saltmed model so that vigor is reduced and the efficiency of water use is enhanced. This is to be achieved by the manipulation of the hydration status of parts of a crop’s roots that could be used to control vegetative vigor without detrimental effects on canopy water relations. The PRD technique is researched for wheat as a winter crop and maize as a summer crop in Egypt. The technique causes the stimulation of physiological responses which are normally associated with water stress and this results in a significant reduction in water use through the production of chemical signals in drying roots. The results confirmed an increase in irrigation water use efficiency using PRD comparing with conventional flood irrigation. The research highly recommends applying the PRD method in the Gulf Cooperation Council (GCC) countries and in new reclaimed areas in Egypt to save water and improve crop quality.展开更多
文摘Leptadenia pyrotechnica is an important multipurpose endangered plant in the Kingdom of Bahrain with restricted distribution. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of indole acetic acid (IAA) and 6-benzylaminopurine (BAP). Initially, 80% and 60% explants responded in direct shoot and callus initiation response respectively in presence of 8.88 μM BAP with 5.71 μM IAA in modified MS media after two weeks of culture. The highest frequency of plant regeneration was observed in presence of 8.88 μM BAP with 1.14 μM IAA following organogenic pathway of differentiation. Hundred percent callus proliferation was observed while initial callus developed in presence of 4.44 μM BAP with 2.85 μM IAA and was transferred in media containing 4.44 μM, 6.66 μM BAP with 2.85 μM IAA and 13.32 μM BAP with 5.71 μM IAA. The callus derived plants were regenerated following the pathway of indirect somatic embryogenesis. The induction of somatic embryogenesis and plant regeneration from callus was also observed in modified MS media supplemented with 4.44 μM BAP and 2.85 μM IAA. The plant regeneration protocol we developed for Leptadenia pyrotechnica will be very beneficial for biodiversity conservation and environment protection of Bahrain. Moreover, the present paper reports for the first time specifically the somatic embryogenesis in this multipurpose desert plant Leptadenia pyrotechnica.
文摘Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conservation of this plant applying tissue culture method was used in the present study. In vitro plant regeneration has been established via shoot organogenesis using nodal meristems. The effect of various plant growth regulators was evaluated to regenerate the whole plants by in vitro micropropagation in modified MS media supplemented with different plant growth regulators (PGRs). In vitro regeneration of plants was involved in three steps by shoot initiation, multiplication and rooting of microshoots. Initially 100% explants of Heliotropium kotschyi responded to initiate shoot in MS medium supplemented with 8.88 μM BAP with 5.71 μM IAA after 4 weeks of culture. Multiplication of shoots and ultimate plant regeneration capacity of Heliotropium kotschyi were compared and the highest performance of 578 shoots/explant was observed in presence of 8.88 μM BAP and 5.71 μM IAA after second transfer. Rooted plantlets were transferred to soil pots and 60% plants were survived after one and a half months. The highly efficient plant regeneration protocol of the desert plant is of great value in rapid plant propagation program for the purpose of conservation of biodiversity.
文摘Partial Rootzone Drying (PRD) is an irrigation technique which offers a means of modifying the growth and development of crops through relatively simple changes to the method of water delivery. The technique causes the stimulation of physiological responses which are normally associated with water stress and this results in a significant reduction in water use through the production of chemical signals in drying roots. Partial drying of one half of the roots of plants grown with two root systems is rapidly translated into a reduction in transpiration and assimilation of all the crop leaves. The aim of this research is to examine and simulate the novel irrigation method (PRD), which would stimulate the endogenous stress response mechanisms of Conocarpus erectus trees in the pilot area of the Arabian Gulf University in Bahrain and wheat and maize crops in the Mashtul Pilot Area (MPA), Egypt using Saltmed model so that vigor is reduced and the efficiency of water use is enhanced. This is to be achieved by the manipulation of the hydration status of parts of a crop’s roots that could be used to control vegetative vigor without detrimental effects on canopy water relations. The PRD technique is researched for wheat as a winter crop and maize as a summer crop in Egypt. The technique causes the stimulation of physiological responses which are normally associated with water stress and this results in a significant reduction in water use through the production of chemical signals in drying roots. The results confirmed an increase in irrigation water use efficiency using PRD comparing with conventional flood irrigation. The research highly recommends applying the PRD method in the Gulf Cooperation Council (GCC) countries and in new reclaimed areas in Egypt to save water and improve crop quality.
