This research involves the preparation ofa biosensor using silicon oxide for biomedical applications, and its effective use for the detection of target DNA hybridization. An electrochemical DNA biosensor was successfu...This research involves the preparation ofa biosensor using silicon oxide for biomedical applications, and its effective use for the detection of target DNA hybridization. An electrochemical DNA biosensor was successfully fabricated by using （3-aminopropyl） tri-ethoxysilane （APTES） as a linker molecule combined with gold nanoparticles （GNPs） on a thermally oxidized 5i02 thin film. The size of the GNPs was calculated by utilizing UV-vis data with an average calculated particle size within the range of 30 - 5 nm, and characterization by transmission electron microscopy （TEM） and atomic force microscopy （AFM）. The GNP-modified SiO2 thin films were electrically characterized through the measurement of capacitance, permittivity and conductivity using a low-cost dielectric analyzer. The capacitance, permittivity and conductivity profiles of the fabricated sensor clearly differentiated DNA immobilization and hybridization.展开更多
基金supported by INEE at (Uni MAP),through the Nano Technology project thereforethe Institute of Nano Electronic Engineering (INEE) at University Malaysia Perlis (Uni MAP) for supporting this work
文摘This research involves the preparation ofa biosensor using silicon oxide for biomedical applications, and its effective use for the detection of target DNA hybridization. An electrochemical DNA biosensor was successfully fabricated by using （3-aminopropyl） tri-ethoxysilane （APTES） as a linker molecule combined with gold nanoparticles （GNPs） on a thermally oxidized 5i02 thin film. The size of the GNPs was calculated by utilizing UV-vis data with an average calculated particle size within the range of 30 - 5 nm, and characterization by transmission electron microscopy （TEM） and atomic force microscopy （AFM）. The GNP-modified SiO2 thin films were electrically characterized through the measurement of capacitance, permittivity and conductivity using a low-cost dielectric analyzer. The capacitance, permittivity and conductivity profiles of the fabricated sensor clearly differentiated DNA immobilization and hybridization.
