BACKGROUND As reported,multiple circular RNAs(circRNAs)interfere with colorectal cancer(CRC)progression.Here,circRNA_0001658(circ_0001658)is focused on studying how it works in CRC.AIM Clarify the expression pattern,b...BACKGROUND As reported,multiple circular RNAs(circRNAs)interfere with colorectal cancer(CRC)progression.Here,circRNA_0001658(circ_0001658)is focused on studying how it works in CRC.AIM Clarify the expression pattern,biological function,and underlying mechanism of circ_0001658 of CRC tumorigenesis.METHODS In CRC-related chip data retrieved using the database named Gene Expression Omnibus,different expressions of circRNAs between CRC and normal tissue samples were identified.Quantitative Real-time PCR and Western blot ensured the analysis on circ_0001658,microRNA-590-5P(miR-590-5p),and methyltransferase-like 3(METTL3)mRNA expressions in tissues and cells.Cell counting kit-8 and flow cytometry were used to detect cell proliferation,apoptosis and migration.The targeting relations between circ_0001658,miR-590-5p,and METTL3 mRNA 3'-untranslated region were under the verification of bioinformatics prediction and dual luciferase-based reporter gene assays.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were employed on the downstream targets of miR-590-5p using the Database for Annotation,Visualization and Integrated Discovery database.RESULTS Circ_0001658 and METTL3 mRNA was elevated in CRC tissues and cells,whereas miR-590-5p was decreased.Circ_0001658 overexpression promoted the proliferation of HT29 cells,inhibited apoptosis,and accelerated the cell cycle.In SW480 cells,knocking down circ_0001658 had the opposite effect.Circ_0001658 could specifically bind to miR-590-5p and negatively modulate its expressions;METTL3 is a miR-590-5p target that can be positively regulated by circ 0001658.Circ 0001658 was inversely associated with miR-590-5p expression while positively with METTL3 expressions.CONCLUSION Circ_0001658 regulates the miR-590-5p/METTL 3-axis to increase CRC cell growth and decrease apoptosis.展开更多
文摘BACKGROUND As reported,multiple circular RNAs(circRNAs)interfere with colorectal cancer(CRC)progression.Here,circRNA_0001658(circ_0001658)is focused on studying how it works in CRC.AIM Clarify the expression pattern,biological function,and underlying mechanism of circ_0001658 of CRC tumorigenesis.METHODS In CRC-related chip data retrieved using the database named Gene Expression Omnibus,different expressions of circRNAs between CRC and normal tissue samples were identified.Quantitative Real-time PCR and Western blot ensured the analysis on circ_0001658,microRNA-590-5P(miR-590-5p),and methyltransferase-like 3(METTL3)mRNA expressions in tissues and cells.Cell counting kit-8 and flow cytometry were used to detect cell proliferation,apoptosis and migration.The targeting relations between circ_0001658,miR-590-5p,and METTL3 mRNA 3'-untranslated region were under the verification of bioinformatics prediction and dual luciferase-based reporter gene assays.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were employed on the downstream targets of miR-590-5p using the Database for Annotation,Visualization and Integrated Discovery database.RESULTS Circ_0001658 and METTL3 mRNA was elevated in CRC tissues and cells,whereas miR-590-5p was decreased.Circ_0001658 overexpression promoted the proliferation of HT29 cells,inhibited apoptosis,and accelerated the cell cycle.In SW480 cells,knocking down circ_0001658 had the opposite effect.Circ_0001658 could specifically bind to miR-590-5p and negatively modulate its expressions;METTL3 is a miR-590-5p target that can be positively regulated by circ 0001658.Circ 0001658 was inversely associated with miR-590-5p expression while positively with METTL3 expressions.CONCLUSION Circ_0001658 regulates the miR-590-5p/METTL 3-axis to increase CRC cell growth and decrease apoptosis.
