Exploring the mechanism of action of bei shashen-maidong in the treatment of non-small cell lung cancer based on network pharmacology and experimental validation

Beishashen(BSS)and Maidong(MD)are commonly used Medicine right for the treatment of non-small cell lung cancer(NSCLC),but their specific mechanism of action is not clear.In this study,network pharmacology and molecular docking techniques were used to investigate the molecular mechanisms of the therapeutic effects of BSS-MD on NSCLC and to experimentally validate some of the targets.The network pharmacology approach,including active ingredient and target screening,drug-compound-target network construction,protein-protein interaction(PPI)network,enrichment analysis,and molecular docking,was used to investigate the mechanism of action of Beisashen and Maitong on NSCLC.First,the active components of BSS-MD and their targets were predicted,of which 423 targets interacted with NSCLC targets.Then,network pharmacology showed that Stigmasterol,Quercetin,Alloisoimperatorin,Isoimperatorin,Beta-sitosterol were the core components of BSS-MD,and PLK1,HSP90AB1,and CDK1 were the key therapeutic targets.KEGG enrichment analysis indicated that the mechanism of action of BSS-MD in NSCLC treatment was related to the cell cycle.Then we further performed experimental validation.CCK-8 assay showed that BSS-MD inhibited LEWIS cell viability and promoted apoptosis in a dose-dependent manner.qPCR assay,immunofluorescence,and protein blotting experiments demonstrated that compared with the control group and the control group,the expression of PLK1,HSP90AB1,and CDK1 mRNAs and proteins were reduced in the treatment group(P<0.01).Therefore,we conclude that BSS-MD can block cell cycle progression by inhibiting the expression of PLK1,CDK1,and HSP90AB1 mRNAs and proteins to inhibit lung cancer cell growth and promote apoptosis,and emphasize that BSS-MD are promising adjuvants for NSCLC treatment.

Research progress of traditional Chinese medicine in regulating tumor microenvironment

The tumor microenvironment(TME)plays a crucial role in facilitating tumorigenesis and progression.Consequently,there is significant research interest within the oncology community in developing interventions that target the TME.Extensive research has been conducted on the mechanism of traditional Chinese medicine(TCM)in tumor therapy,revealing notable similarities between its theoretical framework and that of the TME.TCM has the ability to regulate various components of the microenvironment,including the modulation of proportions of T cell subsets,enhancement of the quantity and activity of NK cells,regulation of polarization of tumor-associated macrophages,suppression of expression of myeloid-derived suppressor cells,reduction of accumulation of tumor-associated endothelial cells,downregulation of the quantity and function of tumor-associated fibroblasts,and modulation of the architecture of the extracellular matrix.These multifaceted interventions ultimately lead to the attainment of anti-tumor objectives.This comprehensive review encompasses a thorough analysis of relevant literature from both domestic and international sources,with a specific emphasis on elucidating the mechanisms through which TCM compound formulas,single drugs,and monomeric components regulate the TME.

H-BONDING SELF-ASSEMBLED TEMPLATE-CONTROLLED SYNTHESIS OF A NOVEL SOLUBLE AND REACTIVE AMIDE-BRIDGED LADDERLIKE POLYHYDROSILOXANE

A novel soluble and reactive amide-bridged ladderlike polyhydrosiloxane （ALPHS） was first synthesized by an amido H-bonding self-assembled template. ALPHS with molecular weight M^-a = 18300 has very highly ordered ladderlike structure, which was confirmed by a sharp resonance absorption peak of [-Si（H）O2/2 ] moiety with the half peak width △1/2 〈 0.5 in^29Si-NMR spectrum. Presence of the reactive Si-H groups gives ALPHS an opportunity to further derive a variety of functional polymers by versatile Si-H reactions such as hydrosilylation, condensation, and so on.

Does persistence of showy calyces in Limonium leptolobum enhance pollinator attraction?

Aims The calyx,the outermost whorl of a flower(usually green),has been considered to function to protect flowers.in some species,how-ever,calyces are colorful and retained during seed development.Limonium species have been exploited as cut flower crops because the calyces persist for several months after the corolla has closed.To explore the adaptive significance of the persistent calyx in a desert plant Limonium leptolobum,we ask whether persistence of caly-ces can enhance pollinator attraction by enlarging floral displays,increasing reproductive success in this self-incompatible species.Methods The yellow flower of L.leptolobum lasted 1-2 days but its white,membranous calyx extended fully after the corolla closed,and per-sisted for over 2 months in the field,making hundreds of‘showy flowers’on one individual.To examine the ecological function of calyces,we test the pollinator attraction hypothesis.in an experi-mental population,we compared the difference in visit frequency and visitor behavior between intact inflorescences and inflores-cences with their calyces removed on the same individual plants.Important findingsin four experimental plots four types of floral visitors were observed including bees,butterflies,syrphid flies and day-flying moths.No significant preference was observed between calyx-free and intact inflorescences for both first arrivals and total visit frequency of all types of floral visitors,indicating that the persistence of calyces did not make plants more attractive to potential pollinators.The pollina-tor attraction hypothesis for the showy calyces was not supported by the current data.Whether the calyx in this desert plant helps seed development or has other functions needs further study.