Supercritical CO_2 technologies have been shown to have greatpotential in pharmaceutical science;they are effective in producing drug and/or polymer particles for biomedical applicationsdue to the mild critical condit...Supercritical CO_2 technologies have been shown to have greatpotential in pharmaceutical science;they are effective in producing drug and/or polymer particles for biomedical applicationsdue to the mild critical conditions(Tc=304.1 K,Pc=7.38MPa),non-toxicity,non-flammability,and low展开更多
Articular cartilage plays an important role in weight-bearing and movement.However,it is also more easily damaged in intra-articular fractures.As the cartilage has no vessel and nerve,so it is hardly to repair itself ...Articular cartilage plays an important role in weight-bearing and movement.However,it is also more easily damaged in intra-articular fractures.As the cartilage has no vessel and nerve,so it is hardly to repair itself once damaged seriously.Tissue engineering is a potential way to cure the cartilage damage because it combines scaffold and cells.Gels展开更多
Nanomaterials doped with non-metallic C have attracted tremendous attention as potential nano-artificial enzymes due to their ability to change the energy band structure to improve their intrinsic properties.Herein,we...Nanomaterials doped with non-metallic C have attracted tremendous attention as potential nano-artificial enzymes due to their ability to change the energy band structure to improve their intrinsic properties.Herein,we report a green,facile,efficient,fast strategy to access high-performance nanozymes via supercritical CO_(2)fluid technology-fabricated polymer nanoreactor of poly-(methyl vinyl ether-co-maleic anhydride)(PVM/MA)coated Co(NO_(3))_(2)into C-doped Co_(3)O_(4)(C-Co_(3)O_(4))nanozyme by a onestep calcination process.Converting PVM/MA to C doping into Co_(3)O_(4)shortens the entire lattice constant of the crystal structure,and the overall valence band energy level below the Fermi level shifts toward the lower energy direction.The as-prepared CCo_(3)O_(4)demonstrated significant peroxidase-like catalytic activity,significantly greater than the undoped Co_(3)O_(4)nanoparticle nanozyme.The following density functional theory(DFT)calculations revealed that the doped nano-enzyme catalytic site displayed a unique electronic structure,altering the material surface with more electrons to fill the anti-bond of the two molecular orbitals,significantly improving the peroxidase-like enzyme catalytic and glucose sensor performance.The resultant enzymatic glucose sensing in a linear range of 0.1–0.6 mM with a detection limit of 3.86μM is in line with standard Michaelis–Menten theory.Collectively,this work demonstrates that converting polymers into nanozymes of C-doped form by supercritical CO_(2)fluid technology in a step is an effective strategy for constructing high-performance glucose sensor nanozymes.This cost-effective,reliable,precise system offers the potential for rapid analyte detection,facilitating its application in a variety of fields.展开更多
Although nano-immunotherapy has advanced dramatically in recent times,there remain two significant hurdles related to immune systems in cancer treatment,such as(namely)inevitable immune elimination of nanoplat-forms a...Although nano-immunotherapy has advanced dramatically in recent times,there remain two significant hurdles related to immune systems in cancer treatment,such as(namely)inevitable immune elimination of nanoplat-forms and severely immunosuppressive microenvironment with low immunogenicity,hampering the perfor-mance of nanomedicines.To address these issues,several immune-regulating camouflaged nanocomposites have emerged as prevailing strategies due to their unique characteristics and specific functionalities.In this review,we emphasize the composition,performances,and mechanisms of various immune-regulating camouflaged nano-platforms,including polymer-coated,cell membrane-camouflaged,and exosome-based nanoplatforms to evade the immune clearance of nanoplatforms or upregulate the immune function against the tumor.Further,we discuss the applications of these immune-regulating camouflaged nanoplatforms in directly boosting cancer immunotherapy and some immunogenic cell death-inducing immunotherapeutic modalities,such as chemo-therapy,photothermal therapy,and reactive oxygen species-mediated immunotherapies,highlighting the cur-rent progress and recent advancements.Finally,we conclude the article with interesting perspectives,suggesting future tendencies of these innovative camouflaged constructs towards their translation pipeline.展开更多
Despite the success,it is highly challenging to battle against pathogenic biofilms-based chronic bacterial infections by conventional antibiotic therapy.Herein,we report a near-infrared(NIR)/acid-induced nanoplatform ...Despite the success,it is highly challenging to battle against pathogenic biofilms-based chronic bacterial infections by conventional antibiotic therapy.Herein,we report a near-infrared(NIR)/acid-induced nanoplatform based on chitosan(CS)-coated indocyanine green(ICG,photosensitizer)/luteolin(LUT,a natural quorum sensing inhibitor)nanocomposites(ICG/LUT-CS)as antibacterial and antibiofilm agents for skin wound healing.Initially,the ICG/LUT nanoplatforms are prepared by the supercritical antisolvent technology and coated with the CS layer.The obtained ICG/LUT-CS with ultra-high encapsulation efficiency exhibited more favorable photothermal conversion effects and improved NIR laser/acid dual-induced drug release behavior than individual modalities,achieving exceptional bacteria-killing and biofilm elimination effects.Moreover,the ICG/LUT-CS realized the synergetic effects of chemotherapy and photothermal therapy outcomes for wound healing.Together,our findings provided an appealing strategy for the rapid preparation and future translational application of ICG/LUT-CS as an ideal agent for fighting against biofilm infections.展开更多
Aripiprazole(ARI),a second-generation atypical antipsychotic drug approved for schizophrenia treatment,shows good efficacy against depression.However,the poorly aqueous solubility of ARI leads to low bioavailability a...Aripiprazole(ARI),a second-generation atypical antipsychotic drug approved for schizophrenia treatment,shows good efficacy against depression.However,the poorly aqueous solubility of ARI leads to low bioavailability and increased dose-related side effects,seriously limiting its application in pharmaceutics.Herein,we demonstrated the fabrication of ARI and poly(methyl vinyl etherco-maleic anhydride)(PVMMA)composite nanoparticles(PA NPs)using the supercritical antisolvent(SAS)process for enhancing its water-solubility and curative anti-depressant effects.Initially,the optimal experimental conditions(ARI/PVMMA mass ratio of 1:6,pressure of 10MPa,and solution flow rate of 0.75ml min^(-1))were determined by a 23 factorial experimental design,resulting in the PA NPs with an excellent particle morphology.In vitro cell experiments showed that PA NPs significantly inhibited the inflammatory response caused by the microglia activation induced by lipopolysaccharide(LPS).Similarly,mice behavioral tests demonstrated that PA NPs significantly improved LPS-induced depression-like behavior.Importantly,compared with free ARI,the LPS-induced activation of microglia in the mouse brain and the expression of inflammatory factors in serum were significantly reduced after treatment with PA NPs.Together,the innovative PA NPs designed by SAS processmight provide a candidate for developing new ARI-based nano-formulations.展开更多
基金Financial supports from Natural Science Foundation of China(8117147,51103049,31170939,31470927 and 31570974)are gratefullyacknowledged
文摘Supercritical CO_2 technologies have been shown to have greatpotential in pharmaceutical science;they are effective in producing drug and/or polymer particles for biomedical applicationsdue to the mild critical conditions(Tc=304.1 K,Pc=7.38MPa),non-toxicity,non-flammability,and low
文摘Articular cartilage plays an important role in weight-bearing and movement.However,it is also more easily damaged in intra-articular fractures.As the cartilage has no vessel and nerve,so it is hardly to repair itself once damaged seriously.Tissue engineering is a potential way to cure the cartilage damage because it combines scaffold and cells.Gels
基金the National Natural Science Foundation of China(Nos.81971734,32071323,and 32271410)Program for Innovative Research Team in Science and Technology in Fujian Province University,Instrumental Analysis Center of Huaqiao University for TEM images,and Subsidized Project for Cultivating Postgraduates’Innovative Ability in Scientific Research of Huaqiao University are gratefully acknowledged。
文摘Nanomaterials doped with non-metallic C have attracted tremendous attention as potential nano-artificial enzymes due to their ability to change the energy band structure to improve their intrinsic properties.Herein,we report a green,facile,efficient,fast strategy to access high-performance nanozymes via supercritical CO_(2)fluid technology-fabricated polymer nanoreactor of poly-(methyl vinyl ether-co-maleic anhydride)(PVM/MA)coated Co(NO_(3))_(2)into C-doped Co_(3)O_(4)(C-Co_(3)O_(4))nanozyme by a onestep calcination process.Converting PVM/MA to C doping into Co_(3)O_(4)shortens the entire lattice constant of the crystal structure,and the overall valence band energy level below the Fermi level shifts toward the lower energy direction.The as-prepared CCo_(3)O_(4)demonstrated significant peroxidase-like catalytic activity,significantly greater than the undoped Co_(3)O_(4)nanoparticle nanozyme.The following density functional theory(DFT)calculations revealed that the doped nano-enzyme catalytic site displayed a unique electronic structure,altering the material surface with more electrons to fill the anti-bond of the two molecular orbitals,significantly improving the peroxidase-like enzyme catalytic and glucose sensor performance.The resultant enzymatic glucose sensing in a linear range of 0.1–0.6 mM with a detection limit of 3.86μM is in line with standard Michaelis–Menten theory.Collectively,this work demonstrates that converting polymers into nanozymes of C-doped form by supercritical CO_(2)fluid technology in a step is an effective strategy for constructing high-performance glucose sensor nanozymes.This cost-effective,reliable,precise system offers the potential for rapid analyte detection,facilitating its application in a variety of fields.
基金Financial support from the National Key Research&Development Program of China(2019YFE0113600)National Natural Science Foundation of China(NSFC,81971734,and 32071323)Program for Innovative Research Team in Science and Technology in Fujian Province University,and the Scientific Research Funds of Huaqiao University(20BS104).
文摘Although nano-immunotherapy has advanced dramatically in recent times,there remain two significant hurdles related to immune systems in cancer treatment,such as(namely)inevitable immune elimination of nanoplat-forms and severely immunosuppressive microenvironment with low immunogenicity,hampering the perfor-mance of nanomedicines.To address these issues,several immune-regulating camouflaged nanocomposites have emerged as prevailing strategies due to their unique characteristics and specific functionalities.In this review,we emphasize the composition,performances,and mechanisms of various immune-regulating camouflaged nano-platforms,including polymer-coated,cell membrane-camouflaged,and exosome-based nanoplatforms to evade the immune clearance of nanoplatforms or upregulate the immune function against the tumor.Further,we discuss the applications of these immune-regulating camouflaged nanoplatforms in directly boosting cancer immunotherapy and some immunogenic cell death-inducing immunotherapeutic modalities,such as chemo-therapy,photothermal therapy,and reactive oxygen species-mediated immunotherapies,highlighting the cur-rent progress and recent advancements.Finally,we conclude the article with interesting perspectives,suggesting future tendencies of these innovative camouflaged constructs towards their translation pipeline.
基金supported by the National Key Research&Development Program of China(2019YFE0113600)the National Natural Science Foundation of China(NSFC 81971734,32071323)and Program for Innovative Research Team in Science,Scientific Research Funds of Huaqiao University(21BS113).
文摘Despite the success,it is highly challenging to battle against pathogenic biofilms-based chronic bacterial infections by conventional antibiotic therapy.Herein,we report a near-infrared(NIR)/acid-induced nanoplatform based on chitosan(CS)-coated indocyanine green(ICG,photosensitizer)/luteolin(LUT,a natural quorum sensing inhibitor)nanocomposites(ICG/LUT-CS)as antibacterial and antibiofilm agents for skin wound healing.Initially,the ICG/LUT nanoplatforms are prepared by the supercritical antisolvent technology and coated with the CS layer.The obtained ICG/LUT-CS with ultra-high encapsulation efficiency exhibited more favorable photothermal conversion effects and improved NIR laser/acid dual-induced drug release behavior than individual modalities,achieving exceptional bacteria-killing and biofilm elimination effects.Moreover,the ICG/LUT-CS realized the synergetic effects of chemotherapy and photothermal therapy outcomes for wound healing.Together,our findings provided an appealing strategy for the rapid preparation and future translational application of ICG/LUT-CS as an ideal agent for fighting against biofilm infections.
基金supported by the National Natural Science Foundation of China(NSFC,81971734,32071323,32271410),the Program for Innovative Research Team in Science and Technology in Fujian Province.
文摘Aripiprazole(ARI),a second-generation atypical antipsychotic drug approved for schizophrenia treatment,shows good efficacy against depression.However,the poorly aqueous solubility of ARI leads to low bioavailability and increased dose-related side effects,seriously limiting its application in pharmaceutics.Herein,we demonstrated the fabrication of ARI and poly(methyl vinyl etherco-maleic anhydride)(PVMMA)composite nanoparticles(PA NPs)using the supercritical antisolvent(SAS)process for enhancing its water-solubility and curative anti-depressant effects.Initially,the optimal experimental conditions(ARI/PVMMA mass ratio of 1:6,pressure of 10MPa,and solution flow rate of 0.75ml min^(-1))were determined by a 23 factorial experimental design,resulting in the PA NPs with an excellent particle morphology.In vitro cell experiments showed that PA NPs significantly inhibited the inflammatory response caused by the microglia activation induced by lipopolysaccharide(LPS).Similarly,mice behavioral tests demonstrated that PA NPs significantly improved LPS-induced depression-like behavior.Importantly,compared with free ARI,the LPS-induced activation of microglia in the mouse brain and the expression of inflammatory factors in serum were significantly reduced after treatment with PA NPs.Together,the innovative PA NPs designed by SAS processmight provide a candidate for developing new ARI-based nano-formulations.