Soybean(Glycine max)is a legume crop with great economic value that provides rich protein and oil for human food and animal feed.In order to cope with the ever-increasing need for soybean products and the changing env...Soybean(Glycine max)is a legume crop with great economic value that provides rich protein and oil for human food and animal feed.In order to cope with the ever-increasing need for soybean products and the changing environment,soybean genetic improvement needs to be accelerated.In recent years,the rapid developed genome editing technologies,such as zinc finger nuclease(ZFNs),transcription activator-like effector nucleases(TALENs),and clustered regularly interspaced short palindromic repeats/CRISPR associated protein(CRISPR/Cas),have shown broad application prospects in gene function research and improvement of important agronomic traits in many crops,and has also brought opportunities for soybean breeding.Here we systematically reviewed recent advances in genome editing technology.We also summarized the significances,current applications,challenges and future perspectives in soybean genome editing,which could provide references for exerting the feature and advantage of this technology to better soybean improvement.展开更多
Auxin, as an important phytohormone, regulates plant growth and development by regula-ting the expression of diverse genes. GH3 is one of the main auxin - responsive genes, and has crucial roles in many...Auxin, as an important phytohormone, regulates plant growth and development by regula-ting the expression of diverse genes. GH3 is one of the main auxin - responsive genes, and has crucial roles in many biology processes. Studies have demonstrated GH3 gene family in various plants, but no ge-nome -wide analysis of the GH3 gene family has been conducted for a legume species. Here we performed a comprehensive genome mining and identified a total of 24 GH3 genes, located on 13 different chromo-somes in the soybean genome （ GmGH3. 1 to GmGH3. 24） . Most of the predicted GmGH3s clustered to-gether in pairs, reflecting the ancient genome duplication event. Analysis of gene structure revealed the presence of introns in protein - coding regions, and that their size, abundance and distribution varied within the gene family. Expression analysis of GmGH3 genes in soybean various tissues of different devel-opmental stages displayed their temporally and spatially expression patterns. We have identified 24 GH3 genes in the soybean genome. The expansion of GmGH3 genes and their distribution pattern on the chro-mosomes revealed genome - wide segmental duplications of soybean. Diverse expression of GmGH3s dur-ing soybean growth and development indicated their important function roles.展开更多
基金supported by the National Genetically Modified Organisms Breeding Major Projects(2006ZX08004-005)。
文摘Soybean(Glycine max)is a legume crop with great economic value that provides rich protein and oil for human food and animal feed.In order to cope with the ever-increasing need for soybean products and the changing environment,soybean genetic improvement needs to be accelerated.In recent years,the rapid developed genome editing technologies,such as zinc finger nuclease(ZFNs),transcription activator-like effector nucleases(TALENs),and clustered regularly interspaced short palindromic repeats/CRISPR associated protein(CRISPR/Cas),have shown broad application prospects in gene function research and improvement of important agronomic traits in many crops,and has also brought opportunities for soybean breeding.Here we systematically reviewed recent advances in genome editing technology.We also summarized the significances,current applications,challenges and future perspectives in soybean genome editing,which could provide references for exerting the feature and advantage of this technology to better soybean improvement.
基金This work was supported by the National Natural Science Foundation of China [ grant number. 31371654, 31522042, 31501655 and 31501334]； National Trans-genic Project [ grant number. 2014ZX08004003, 2015ZX08004003 and 2016ZX08004003 ]；Agricultur-al Science and Technology Innovation Program, Breed-ing project [grant number. SQ2016ZY03002375 ] ； Wuhan Chenguang Plan [grant number. 2015070404-010193].
文摘Auxin, as an important phytohormone, regulates plant growth and development by regula-ting the expression of diverse genes. GH3 is one of the main auxin - responsive genes, and has crucial roles in many biology processes. Studies have demonstrated GH3 gene family in various plants, but no ge-nome -wide analysis of the GH3 gene family has been conducted for a legume species. Here we performed a comprehensive genome mining and identified a total of 24 GH3 genes, located on 13 different chromo-somes in the soybean genome （ GmGH3. 1 to GmGH3. 24） . Most of the predicted GmGH3s clustered to-gether in pairs, reflecting the ancient genome duplication event. Analysis of gene structure revealed the presence of introns in protein - coding regions, and that their size, abundance and distribution varied within the gene family. Expression analysis of GmGH3 genes in soybean various tissues of different devel-opmental stages displayed their temporally and spatially expression patterns. We have identified 24 GH3 genes in the soybean genome. The expansion of GmGH3 genes and their distribution pattern on the chro-mosomes revealed genome - wide segmental duplications of soybean. Diverse expression of GmGH3s dur-ing soybean growth and development indicated their important function roles.
