Early detection and intervention are key strategies to reduce mortality,increase longterm survival,and improve the therapeutic effects of hepatocellular carcinoma(HCC)patients.Herein,the isobaric tag for relative and ...Early detection and intervention are key strategies to reduce mortality,increase longterm survival,and improve the therapeutic effects of hepatocellular carcinoma(HCC)patients.Herein,the isobaric tag for relative and absolute quantitation(iTRAQ)-based quantitative proteomic strategy was used to study the secretomes in conditioned media from HCC cancerous tissues,surrounding noncancerous tissues,and distal noncancerous tissues to identify diagnostic and prognostic biomarkers for HCC.In total,22 and 49 dysregulated secretory proteins were identified in the cancerous and surrounding noncancerous tissues,respectively,compared with the distal noncancerous tissues.Among these proteins,carbonic anhydrase II(CA2)was identified to be significantly upregulated in the secretome of cancerous tissues;correspondingly,the serum concentrations of CA2 were remarkably increased in HCC patients compared with that in normal populations.Interestingly,a significant increase of serum CA2 in recurrent HCC patients after radical resection was also confirmed compared with HCC patients without recurrence,and the serum level of CA2 could act as an independent prognostic factor for time to recurrence and overall survival.Regarding the mechanism,the secreted CA2 enhances the migration and invasion of HCC cells by activating the epithelial mesenchymal transition pathway.Taken together,this study identified a novel biomarker for HCC diagnosis and prognosis,and provided a valuable resource of HCC secretome for investigating serological biomarkers.展开更多
The Jin-Neelin model for the El Nio–Southern Oscillation(ENSO for short) is considered for which the authors establish existence and uniqueness of global solutions in time over an unbounded channel domain. The resu...The Jin-Neelin model for the El Nio–Southern Oscillation(ENSO for short) is considered for which the authors establish existence and uniqueness of global solutions in time over an unbounded channel domain. The result is proved for initial data and forcing that are sufficiently small. The smallness conditions involve in particular key physical parameters of the model such as those that control the travel time of the equatorial waves and the strength of feedback due to vertical-shear currents and upwelling; central mechanisms in ENSO dynamics.From the mathematical view point, the system appears as the coupling of a linear shallow water system and a nonlinear heat equation. Because of the very different nature of the two components of the system, the authors find it convenient to prove the existence of solution by semi-discretization in time and utilization of a fractional step scheme. The main idea consists of handling the coupling between the oceanic and temperature components by dividing the time interval into small sub-intervals of length k and on each sub-interval to solve successively the oceanic component, using the temperature T calculated on the previous sub-interval, to then solve the sea-surface temperature(SST for short) equation on the current sub-interval. The passage to the limit as k tends to zero is ensured via a priori estimates derived under the aforementioned smallness conditions.展开更多
We address the well-posedness of the 2D(Euler)–Boussinesq equations with zero viscosity and positive diffusivity in the polygonal-like domains with Yudovich’s type data,which gives a positive answer to part of the q...We address the well-posedness of the 2D(Euler)–Boussinesq equations with zero viscosity and positive diffusivity in the polygonal-like domains with Yudovich’s type data,which gives a positive answer to part of the questions raised in Lai(Arch Ration Mech Anal 199(3):739–760,2011).Our analysis on the the polygonallike domains essentially relies on the recent elliptic regularity results for such domains proved in Bardos et al.(J Math Anal Appl 407(1):69–89,2013)and Di Plinio(SIAM J Math Anal 47(1):159–178,2015).展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.81702910 and 81672376)the Educational Commission of Fujian Province,China(Grant No.2018B013)the Natural Science Foundation of Fujian Province,China(Grant Nos.2019J01298,2017J01159,and 2016J01417)
文摘Early detection and intervention are key strategies to reduce mortality,increase longterm survival,and improve the therapeutic effects of hepatocellular carcinoma(HCC)patients.Herein,the isobaric tag for relative and absolute quantitation(iTRAQ)-based quantitative proteomic strategy was used to study the secretomes in conditioned media from HCC cancerous tissues,surrounding noncancerous tissues,and distal noncancerous tissues to identify diagnostic and prognostic biomarkers for HCC.In total,22 and 49 dysregulated secretory proteins were identified in the cancerous and surrounding noncancerous tissues,respectively,compared with the distal noncancerous tissues.Among these proteins,carbonic anhydrase II(CA2)was identified to be significantly upregulated in the secretome of cancerous tissues;correspondingly,the serum concentrations of CA2 were remarkably increased in HCC patients compared with that in normal populations.Interestingly,a significant increase of serum CA2 in recurrent HCC patients after radical resection was also confirmed compared with HCC patients without recurrence,and the serum level of CA2 could act as an independent prognostic factor for time to recurrence and overall survival.Regarding the mechanism,the secreted CA2 enhances the migration and invasion of HCC cells by activating the epithelial mesenchymal transition pathway.Taken together,this study identified a novel biomarker for HCC diagnosis and prognosis,and provided a valuable resource of HCC secretome for investigating serological biomarkers.
基金supported by the Office of Naval Research Multidisciplinary University Research Initiative(No.N00014-16-1-2073)the National Science Foundation(Nos.OCE-1658357,DMS-1616981,DMS-1206438,DMS-1510249)the Research Fund of Indiana University
文摘The Jin-Neelin model for the El Nio–Southern Oscillation(ENSO for short) is considered for which the authors establish existence and uniqueness of global solutions in time over an unbounded channel domain. The result is proved for initial data and forcing that are sufficiently small. The smallness conditions involve in particular key physical parameters of the model such as those that control the travel time of the equatorial waves and the strength of feedback due to vertical-shear currents and upwelling; central mechanisms in ENSO dynamics.From the mathematical view point, the system appears as the coupling of a linear shallow water system and a nonlinear heat equation. Because of the very different nature of the two components of the system, the authors find it convenient to prove the existence of solution by semi-discretization in time and utilization of a fractional step scheme. The main idea consists of handling the coupling between the oceanic and temperature components by dividing the time interval into small sub-intervals of length k and on each sub-interval to solve successively the oceanic component, using the temperature T calculated on the previous sub-interval, to then solve the sea-surface temperature(SST for short) equation on the current sub-interval. The passage to the limit as k tends to zero is ensured via a priori estimates derived under the aforementioned smallness conditions.
文摘We address the well-posedness of the 2D(Euler)–Boussinesq equations with zero viscosity and positive diffusivity in the polygonal-like domains with Yudovich’s type data,which gives a positive answer to part of the questions raised in Lai(Arch Ration Mech Anal 199(3):739–760,2011).Our analysis on the the polygonallike domains essentially relies on the recent elliptic regularity results for such domains proved in Bardos et al.(J Math Anal Appl 407(1):69–89,2013)and Di Plinio(SIAM J Math Anal 47(1):159–178,2015).
