In this study, the liver, kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District, Taizhou City for virus isolation and identification. The isolated virus was inoculated...In this study, the liver, kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District, Taizhou City for virus isolation and identification. The isolated virus was inoculated onto PK15 monolayer cells. The virus culture was collected to extract genomic DNA for PCR assay and indirect immunoinfluscent assay. The results showed that the isolated virus was porcine pseudorabies virus, which was named TAIZ130417. The growth titer of the isolated virus reached 10 8.12 TCID 50 /ml on PK15 cells. Rabbits inoculated with the isolated virus soon exhibited pseudorabies symptoms such as itching and eventually died. The results provided reference for in-depth research and scientific prevention and control of pseudorabies.展开更多
In September 2011, an infectious disease suspected to be postweaning multisystemic wasting syndrome (PMWS) broke out in some pig farm in Taizhou. The inguinal lymph node, liver and lung tissues were collected and gr...In September 2011, an infectious disease suspected to be postweaning multisystemic wasting syndrome (PMWS) broke out in some pig farm in Taizhou. The inguinal lymph node, liver and lung tissues were collected and grinded into tissue suspension. The suspension was subjected to PCR detection, and the positive product was sequenced. The suspension of positive samples was filtered with 0.22 μm filter membrane, and the filtrate was inoculated onto PK15 cells. After five generations of blind passages, the cell viral liquid was collected and extracted for DNA, which was subjected to PCR detection and indirect immunofluorescence. The results showed that the isolate was porcine circovirus type 2 (PCV2) and designated as TAIZ110926. The target sequence was committed to NCBI with a serial number: KF039888.展开更多
OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertili...OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertility were randomized into two groups:30 in an experimental group treated with Xiaoyao powder(Shugan)plus gonadotropin-releasing hormone analog(GnRHa)/follicle-stimulating hormone(FSH)/human chorionic gonadotropin(hCG)and 28 in the control group who were treated with GnRHa/FSH/hCG only.The total gonadotropin(Gn)doses required,endometrial thickness,oocyte numbers,high quality embryo production rate and pregnancy rate of the two groups were compared.The concentration of growth differentiation factor-9(GDF-9)in follicular fluid was detected by western blotting and the expression of GDF-9 mRNA in granulosa cells was measured using reverse tran-scription-polymerase chain reaction amplification.RESULTS:In the experimental group,the Gn dose was significantly lower than that in the control group;the endometrial thickness,high quality embryo production and pregnancy rates were significantly higher and the expression of GDF-9 mRNA was also significantly higher than in the control group(all P<0.05).CONCLUSION:Shugan treatment can improve the pregnancy rate of women with tubal infertility;its mechanism is possibly related to the increased expression of GDF-9 in granulosa cells.展开更多
RAD9 regulates multiple cellular processes that influence genomic integrity,and for at least some of its functions the protein acts as part of a heterotrimeric complex bound to HUS1 and RAD1 proteins.RAD9 participates...RAD9 regulates multiple cellular processes that influence genomic integrity,and for at least some of its functions the protein acts as part of a heterotrimeric complex bound to HUS1 and RAD1 proteins.RAD9 participates in DNA repair,including base excision repair,homologous recombination repair and mismatch repair,multiple cell cycle phase checkpoints and apoptosis.In addition,functions including the transactivation of downstream target genes,immunoglobulin class switch recombination,as well as 3′–5′exonuclease activity have been reported.Aberrant RAD9 expression has been linked to breast,lung,thyroid,skin and prostate tumorigenesis,and a cause–effect relationship has been demonstrated for the latter two.Interestingly,human RAD9 overproduction correlates with prostate cancer whereas deletion of Mrad9,the corresponding mouse gene,in keratinocytes leads to skin cancer.These results reveal that RAD9 protein can function as an oncogene or tumor suppressor,and aberrantly high or low levels can have deleterious health consequences.It is not clear which of the many functions of RAD9 is critical for carcinogenesis,but several alternatives are considered herein and implications for the development of novel cancer therapies based on these findings are examined.展开更多
In this paper, size-controlled and surface-functionalized RhB-labeled and Cyclosporin A (CsA)-loaded polysty- rene (PS) nanospheres were successfully synthesized via miniemulsion polymerization. The biophysical pr...In this paper, size-controlled and surface-functionalized RhB-labeled and Cyclosporin A (CsA)-loaded polysty- rene (PS) nanospheres were successfully synthesized via miniemulsion polymerization. The biophysical properties of PEG functionalized PS nanospheres from protein adsorption, blood compatibility, cell compatibility and cell penetrability showed the nanoparticles with high biocompatibility. These results indicated that PEG modified PS nanospheres showed outstanding properties as low size distribution (0.164), high encapsulation efficiency (98.3%), long re-calcification time (50% than positive control), low hemolysis ratio (3.19%) and high cell viability (95.3%). This work could be used as a good drug delivery system for CsA.展开更多
This study is to prepare a nanosuspension based on poly(lactic-co-glycolic acid) (PLGA) for delivery, controlled release and enhanced anti-solid tumor effects of As2O3. As2O3-1oaded PLGA nanocapsules (As2O3-PLGA...This study is to prepare a nanosuspension based on poly(lactic-co-glycolic acid) (PLGA) for delivery, controlled release and enhanced anti-solid tumor effects of As2O3. As2O3-1oaded PLGA nanocapsules (As2O3-PLGANCs) were prepared by double emulsion-solvent evaporation method and were optimized by univariate analysis in combination with orthogonal experimental according to several factors. The optimized As2O3-PLGA NCs presented suitable physical stability, favorable size of (200.2± 10.6) nm (PDI=0.117 ±0.008), spherical shape, and high encapsulation efficiency (92.48%± 2.14%). The in vitro suspension stability of the NCs was excellent. The release of As2O3 from the NCs showed pH responsive release characteristics. The NCs can be efficiently taken up by SMMC-7721 cell and showed excellent antitumor efficacy against SMMC-7721 cell line. Then, As2O3-PLGA NCs could be considered as a promising formulation for the pH dependent release of As2O3 in cancer cells and enhance the anti-solid tumor effects of As2O3.展开更多
Bioactive glasses (BGs) are ideal materials for macroporous scaffolds due to their excellent osteoconductive, osteoinductive, biocompatible and biodegradable properties, and their high bone bonding rates. Macroporou...Bioactive glasses (BGs) are ideal materials for macroporous scaffolds due to their excellent osteoconductive, osteoinductive, biocompatible and biodegradable properties, and their high bone bonding rates. Macroporous scaffolds made From BGs are in high demand for bone regeneration because they can stimulate vascularized bone ingrowth and they enhance bonding between scaffolds and surrounding tissues. Engineering BG/biopolymers (BP) composites or hybrids may be a good way to prepare macroporous scaffolds with excellent properties. This paper summarizes the progress in the past few years in preparing three-dimensional macroporous BG and BG/BP scaffolds for bone regeneration. Since the brittleness of BGs is a major problem in developing macroporous scaffolds and this limits their use in load bearing applications, the mechanical properties of macroporous scaffolds are particularly emphasized in this review.展开更多
基金Supported by Youth Fund of Jiangsu Agri-animal Husbandry Vocational College(NSFQN1304)Key Project of Jiangsu Agri-animal Husbandry Vocational College(ZD201104)Phoenix Talent Project of Jiangsu Agri-animal Husbandry Vocational College(10434014001)
文摘In this study, the liver, kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District, Taizhou City for virus isolation and identification. The isolated virus was inoculated onto PK15 monolayer cells. The virus culture was collected to extract genomic DNA for PCR assay and indirect immunoinfluscent assay. The results showed that the isolated virus was porcine pseudorabies virus, which was named TAIZ130417. The growth titer of the isolated virus reached 10 8.12 TCID 50 /ml on PK15 cells. Rabbits inoculated with the isolated virus soon exhibited pseudorabies symptoms such as itching and eventually died. The results provided reference for in-depth research and scientific prevention and control of pseudorabies.
基金Supported by Key Project of Jiangsu Agri-animal Husbandry Vocational College(ZD201104)College Students'Innovation and Enterpreneurship Training Program of Jiangsu Province(201312806014Y)Special Fund for Fenghuang talents in Jiangsu Agri-animal Husbandry Vocational College
文摘In September 2011, an infectious disease suspected to be postweaning multisystemic wasting syndrome (PMWS) broke out in some pig farm in Taizhou. The inguinal lymph node, liver and lung tissues were collected and grinded into tissue suspension. The suspension was subjected to PCR detection, and the positive product was sequenced. The suspension of positive samples was filtered with 0.22 μm filter membrane, and the filtrate was inoculated onto PK15 cells. After five generations of blind passages, the cell viral liquid was collected and extracted for DNA, which was subjected to PCR detection and indirect immunofluorescence. The results showed that the isolate was porcine circovirus type 2 (PCV2) and designated as TAIZ110926. The target sequence was committed to NCBI with a serial number: KF039888.
基金Supported by the National Natural Science Foundation of China(No.81173294)
文摘OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertility were randomized into two groups:30 in an experimental group treated with Xiaoyao powder(Shugan)plus gonadotropin-releasing hormone analog(GnRHa)/follicle-stimulating hormone(FSH)/human chorionic gonadotropin(hCG)and 28 in the control group who were treated with GnRHa/FSH/hCG only.The total gonadotropin(Gn)doses required,endometrial thickness,oocyte numbers,high quality embryo production rate and pregnancy rate of the two groups were compared.The concentration of growth differentiation factor-9(GDF-9)in follicular fluid was detected by western blotting and the expression of GDF-9 mRNA in granulosa cells was measured using reverse tran-scription-polymerase chain reaction amplification.RESULTS:In the experimental group,the Gn dose was significantly lower than that in the control group;the endometrial thickness,high quality embryo production and pregnancy rates were significantly higher and the expression of GDF-9 mRNA was also significantly higher than in the control group(all P<0.05).CONCLUSION:Shugan treatment can improve the pregnancy rate of women with tubal infertility;its mechanism is possibly related to the increased expression of GDF-9 in granulosa cells.
基金supported by National Institutes of Health grants CA130536,CA049062,ES017557 and GM079107.
文摘RAD9 regulates multiple cellular processes that influence genomic integrity,and for at least some of its functions the protein acts as part of a heterotrimeric complex bound to HUS1 and RAD1 proteins.RAD9 participates in DNA repair,including base excision repair,homologous recombination repair and mismatch repair,multiple cell cycle phase checkpoints and apoptosis.In addition,functions including the transactivation of downstream target genes,immunoglobulin class switch recombination,as well as 3′–5′exonuclease activity have been reported.Aberrant RAD9 expression has been linked to breast,lung,thyroid,skin and prostate tumorigenesis,and a cause–effect relationship has been demonstrated for the latter two.Interestingly,human RAD9 overproduction correlates with prostate cancer whereas deletion of Mrad9,the corresponding mouse gene,in keratinocytes leads to skin cancer.These results reveal that RAD9 protein can function as an oncogene or tumor suppressor,and aberrantly high or low levels can have deleterious health consequences.It is not clear which of the many functions of RAD9 is critical for carcinogenesis,but several alternatives are considered herein and implications for the development of novel cancer therapies based on these findings are examined.
基金the National Natural Science Foundation of China (Nos. 51073133, 21201149), the University Scientific Research Project in Jiangsu Province (No. 14KJA430006), the Natural Sci- ence Foundation of Jiangsu Province (No. BK2012259), the Combination of Innovative Funding-forward- looking Joint Research Project (No. SBY2014020171) and the Priority Academic Program Development of Jiangsu Higher Education Institutions. The detection was supported by the Testing Center of Yangzhou University.
文摘In this paper, size-controlled and surface-functionalized RhB-labeled and Cyclosporin A (CsA)-loaded polysty- rene (PS) nanospheres were successfully synthesized via miniemulsion polymerization. The biophysical properties of PEG functionalized PS nanospheres from protein adsorption, blood compatibility, cell compatibility and cell penetrability showed the nanoparticles with high biocompatibility. These results indicated that PEG modified PS nanospheres showed outstanding properties as low size distribution (0.164), high encapsulation efficiency (98.3%), long re-calcification time (50% than positive control), low hemolysis ratio (3.19%) and high cell viability (95.3%). This work could be used as a good drug delivery system for CsA.
基金This research was supported by the National Natural Science Foundation of China (No. 21201149), the Natural Science Foundation of Jiangsu Province (No. BK2012259), the Project of the Testing Center of Yangzhou University (China), the Postdoctoral Science Foundation and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘This study is to prepare a nanosuspension based on poly(lactic-co-glycolic acid) (PLGA) for delivery, controlled release and enhanced anti-solid tumor effects of As2O3. As2O3-1oaded PLGA nanocapsules (As2O3-PLGANCs) were prepared by double emulsion-solvent evaporation method and were optimized by univariate analysis in combination with orthogonal experimental according to several factors. The optimized As2O3-PLGA NCs presented suitable physical stability, favorable size of (200.2± 10.6) nm (PDI=0.117 ±0.008), spherical shape, and high encapsulation efficiency (92.48%± 2.14%). The in vitro suspension stability of the NCs was excellent. The release of As2O3 from the NCs showed pH responsive release characteristics. The NCs can be efficiently taken up by SMMC-7721 cell and showed excellent antitumor efficacy against SMMC-7721 cell line. Then, As2O3-PLGA NCs could be considered as a promising formulation for the pH dependent release of As2O3 in cancer cells and enhance the anti-solid tumor effects of As2O3.
文摘Bioactive glasses (BGs) are ideal materials for macroporous scaffolds due to their excellent osteoconductive, osteoinductive, biocompatible and biodegradable properties, and their high bone bonding rates. Macroporous scaffolds made From BGs are in high demand for bone regeneration because they can stimulate vascularized bone ingrowth and they enhance bonding between scaffolds and surrounding tissues. Engineering BG/biopolymers (BP) composites or hybrids may be a good way to prepare macroporous scaffolds with excellent properties. This paper summarizes the progress in the past few years in preparing three-dimensional macroporous BG and BG/BP scaffolds for bone regeneration. Since the brittleness of BGs is a major problem in developing macroporous scaffolds and this limits their use in load bearing applications, the mechanical properties of macroporous scaffolds are particularly emphasized in this review.