Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments,such as high-glucose,excessive reactive oxygen species(ROS),hypoxia and malnutrition.Unfortunately,few stra...Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments,such as high-glucose,excessive reactive oxygen species(ROS),hypoxia and malnutrition.Unfortunately,few strategies have been developed to ameliorate the multiple microenvironments simultaneously.In this study,Chlorella sp.(Chlorella)hydrogels were prepared against diabetic wounds.In vitro experiments demonstrated that living Chlorella could produce dissolved oxygen by photosynthesis,actively consume glucose and deplete ROS with the inherent antioxidants,during the daytime.At night,Chlorella was inactivated in situ by chlorine dioxide with human-body harmless concentration to utilize its abundant contents.It was verified in vitro that the inactivated-Chlorella could supply nutrition,relieve inflammation and terminate the oxygen-consumption of Chlorella-respiration.The advantages of living Chlorella and its contents were integrated ingeniously.The abovementioned functions were proven to accelerate cell proliferation,migration and angiogenesis in vitro.Then,streptozotocininduced diabetic mice were employed for further validation.The in vivo outcomes confirmed that Chlorella could ameliorate the undesirable microenvironments,including hypoxia,high-glucose,excessiveROS and chronic inflammation,thereby synergistically promoting tissue regeneration.Given the results above,Chlorella is considered as a tailor-made therapeutic strategy for diabetic wound healing.展开更多
基金financially supported by the National Natural Science Foundation of China(81673830)Six Talent Peaks Project in Jiangsu Province(YY053,China)+1 种基金Major Project and Double firstclass innovative team(CPU2018GY28,China)National Science and Technology Major Project(2017zx09101001005,China)。
文摘Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments,such as high-glucose,excessive reactive oxygen species(ROS),hypoxia and malnutrition.Unfortunately,few strategies have been developed to ameliorate the multiple microenvironments simultaneously.In this study,Chlorella sp.(Chlorella)hydrogels were prepared against diabetic wounds.In vitro experiments demonstrated that living Chlorella could produce dissolved oxygen by photosynthesis,actively consume glucose and deplete ROS with the inherent antioxidants,during the daytime.At night,Chlorella was inactivated in situ by chlorine dioxide with human-body harmless concentration to utilize its abundant contents.It was verified in vitro that the inactivated-Chlorella could supply nutrition,relieve inflammation and terminate the oxygen-consumption of Chlorella-respiration.The advantages of living Chlorella and its contents were integrated ingeniously.The abovementioned functions were proven to accelerate cell proliferation,migration and angiogenesis in vitro.Then,streptozotocininduced diabetic mice were employed for further validation.The in vivo outcomes confirmed that Chlorella could ameliorate the undesirable microenvironments,including hypoxia,high-glucose,excessiveROS and chronic inflammation,thereby synergistically promoting tissue regeneration.Given the results above,Chlorella is considered as a tailor-made therapeutic strategy for diabetic wound healing.
