Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong, Chinagrains to a^1-martensite nanograins with bimodal grain size distribution for lower strain rates to nanotwins ...Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong, Chinagrains to a^1-martensite nanograins with bimodal grain size distribution for lower strain rates to nanotwins in the ultrafine/coarse grained austenite phase for higher strain rates. Meanwhile, we will further address the mechanism-based plastic models to describe the yield strength, strain hardening and ductility in nanostructured metals with bimodal grain size distribution and nanotwinned polycrystalline metals. The proposed theoretical models can comprehensively describe the plastic deformation in these two kinds of nanostructured metals and excellent agreement is achieved between the numerical and experimental results. These models can be utilized to optimize the strength and ductility in nanostructured metals by controlling the size and distribution of nanostructures.展开更多
Metabolic reprogramming is a common phenomenon in cancer,with aerobic glycolysis being one of its important characteristics.Hypoxia-inducible factor-1α(HIF1Α)is thought to play an important role in aerobic glycolysi...Metabolic reprogramming is a common phenomenon in cancer,with aerobic glycolysis being one of its important characteristics.Hypoxia-inducible factor-1α(HIF1Α)is thought to play an important role in aerobic glycolysis.Meanwhile,naringin is a natural flavanone glycoside derived from grapefruits and many other citrus fruits.In this work,we identified glycolytic genes related to HIF1Αby analyzing the colon cancer database.The analysis of extracellular acidification rate and cell function verified the regulatory effects of HIF1Αoverexpression on glycolysis,and the proliferation and migration of colon cancer cells.Moreover,naringin was used as an inhibitor of colon cancer cells to illustrate its effect on HIF1Αfunction.The results showed that the HIF1Αand enolase 2(ENO2)levels in colon cancer tissues were highly correlated,and their high expression indicated a poor prognosis for colon cancer patients.Mechanistically,HIF1Αdirectly binds to the DNA promoter region and upregulates the transcription of ENO2;ectopic expression of ENO2 increased aerobic glycolysis in colon cancer cells.Most importantly,we found that the appropriate concentration of naringin inhibited the transcriptional activity of HIF1Α,which in turn decreased aerobic glycolysis in colon cancer cells.Generally,naringin reduces glycolysis in colon cancer cells by reducing the transcriptional activity of HIF1Αand the proliferation and invasion of colon cancer cells.This study helps to elucidate the relationship between colon cancer progression and glucose metabolism,and demonstrates the efficacy of naringin in the treatment of colon cancer.展开更多
基金supportedby the Chinese Ministry of Science and Technology of China (2012CB932203)the Research Grants Council of the Hong Kong Special Administrative Region of China(CityU8/CRF/08 and GRF/CityU519110)the Croucher Foundation CityU9500006 and PolyU Postdoctoral Fellowship Project (G-YX3S)
文摘Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong, Chinagrains to a^1-martensite nanograins with bimodal grain size distribution for lower strain rates to nanotwins in the ultrafine/coarse grained austenite phase for higher strain rates. Meanwhile, we will further address the mechanism-based plastic models to describe the yield strength, strain hardening and ductility in nanostructured metals with bimodal grain size distribution and nanotwinned polycrystalline metals. The proposed theoretical models can comprehensively describe the plastic deformation in these two kinds of nanostructured metals and excellent agreement is achieved between the numerical and experimental results. These models can be utilized to optimize the strength and ductility in nanostructured metals by controlling the size and distribution of nanostructures.
基金supported by the Fund of Hubei Provincial Health Commission(No.ZY2021M080)the Medical Research Project of Jiangsu Provincial Health Commission(No.Z2021068)+1 种基金the Yancheng Medical Science and Technology Development Plan Project(No.YK2021004)the Young Scientific and Technological Talents Support Project by Jiangsu Association for Science and Technology(No.TJ-2022-097),China.
文摘Metabolic reprogramming is a common phenomenon in cancer,with aerobic glycolysis being one of its important characteristics.Hypoxia-inducible factor-1α(HIF1Α)is thought to play an important role in aerobic glycolysis.Meanwhile,naringin is a natural flavanone glycoside derived from grapefruits and many other citrus fruits.In this work,we identified glycolytic genes related to HIF1Αby analyzing the colon cancer database.The analysis of extracellular acidification rate and cell function verified the regulatory effects of HIF1Αoverexpression on glycolysis,and the proliferation and migration of colon cancer cells.Moreover,naringin was used as an inhibitor of colon cancer cells to illustrate its effect on HIF1Αfunction.The results showed that the HIF1Αand enolase 2(ENO2)levels in colon cancer tissues were highly correlated,and their high expression indicated a poor prognosis for colon cancer patients.Mechanistically,HIF1Αdirectly binds to the DNA promoter region and upregulates the transcription of ENO2;ectopic expression of ENO2 increased aerobic glycolysis in colon cancer cells.Most importantly,we found that the appropriate concentration of naringin inhibited the transcriptional activity of HIF1Α,which in turn decreased aerobic glycolysis in colon cancer cells.Generally,naringin reduces glycolysis in colon cancer cells by reducing the transcriptional activity of HIF1Αand the proliferation and invasion of colon cancer cells.This study helps to elucidate the relationship between colon cancer progression and glucose metabolism,and demonstrates the efficacy of naringin in the treatment of colon cancer.