Optimization of Ultrasonic Extraction Process of Oenanthe benghalensis Polysaccharide by Orthogonal Test

[Objectives] The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test. [Methods] Phenol-sulfuric acid method was used to determine polysaccharide's content. Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected. [Results] The influence order of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide was A > D > B > C. Via the orthogonal test,it was determined that the optimal extraction condition of O. benghalensis polysaccharide was as below: solid-liquid ratio 1∶ 25( g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min. Under the condition,extraction rate of O. benghalensis polysaccharide was9. 80%. [Conclusions] The process could provide the reference basis for extracting and producing O. benghalensis polysaccharides.

Extraction of Flavonoids from Leaves of Coix lacryma-jobi L.

[Objectives] To extract the flavonoids from leaves of Coix lacryma-jobi L. [Methods] Ethanol extraction method was adopted,spectrophotometry was used,and single factor experiment and orthogonal experiment were carried out to study the effects of ethanol percentage,extraction temperature,solid-to-liquid ratio and extraction time on the extraction of total flavonoids from leaves of C. lacryma-jobi L.[Results] The order of 4 factors influencing the extraction of flavonoids from leaves of C. lacryma-jobi L. was: solid-to-liquid ratio > extraction time > ethanol percentage > extraction temperature. When the extraction temperature was 70℃,the extraction time was 1. 5 h and the solid-liquid ratio was 1: 10,the ethanol percentage was 60%,the extraction effect was the best,extraction of flavonoids was 0. 107 5 mg/m L.[Conclusions] This study is expected to provide a theoretical basis for further development and utilization of C. lacryma-jobi L.