神经退行性疾病是人类最难治疗的疾病之一.易获取的间充质干细胞(MSCs)可用于自体干细胞移植,间充质干细胞治疗被认为是最有希望的治疗选择之一.然而,传统的生长诱导因子难以实现间充质干细胞的神经分化.电刺激方式可以诱导间充质干细...神经退行性疾病是人类最难治疗的疾病之一.易获取的间充质干细胞(MSCs)可用于自体干细胞移植,间充质干细胞治疗被认为是最有希望的治疗选择之一.然而,传统的生长诱导因子难以实现间充质干细胞的神经分化.电刺激方式可以诱导间充质干细胞的神经分化,但外部电线和复杂的设备给临床治疗带来了很大阻碍.在这项研究中,基于磁电感应效应,我们发现在无需任何生物或化学因子的辅助下,旋转磁场(RMF)驱动下还原氧化石墨烯膜(rGO-M)上产生的无线电信号可以诱导间充质干细胞的神经元样分化.体外实验结果显示,RMF以400 r min^(-1)的速度刺激rGO-M,每天刺激15 min,rGO-M上的MSCs能表达神经元特异性基因和蛋白,连续处理15天后,基因和蛋白的表达量得到明显提升.大鼠体内实验证实,rGO-M上的外源性间充质干细胞可以在旋转磁场的驱动下分化成神经元样细胞.鉴于rGO-M和自体间充质干细胞来源成本较低,rGO-M介导的无线电刺激方法为神经退行性疾病的干细胞治疗提供了一个可行的方案.展开更多
Mesenchymal stem cells(MSCs)are multi-potent cells that are able to differentiate and mature into various types of cells under a certain microenvironment for cell therapy and tissue regeneration.Scandium(Sc),an import...Mesenchymal stem cells(MSCs)are multi-potent cells that are able to differentiate and mature into various types of cells under a certain microenvironment for cell therapy and tissue regeneration.Scandium(Sc),an important rare earth element,recently has been intensively investigated in biomedical fields as well as industrial engineering,and chloride channels have been proven to be able to affect osteogenic differentiation.Thus,it is significant to investigate effects of ScCl_(3)on cell activities of MSCs.In this paper,rat bone MSCs(rBMSCs)were co-cultured with various concentrations of ScCl_(3)(1×10^(-8),1×10^(-6),and 1×10^(-4)mol/L)to evaluate their influence on cell proliferation as well as osteogenic and adipogenic differentiation in vitro.The results indicate that ScCl_(3)promotes the proliferation of rBMSCs initially,which is yet reduced upon ion accumulation.We used immunofluorescence staining,quantitative real time polymerase chain reactions,and assays measuring alkaline phosphatase activity,mineralized deposits,and intracytoplasmic lipids to reveal that rBMSCs treated with ScCl_(3)at concentrations of 1×10^(-8)-1×10^(-6)mol/L can enhance levels of osteogenic differentiation in a dosedependent manner and reduce adipogenic differentiation to a certain degree through Wnt/β-catenin signaling pathway.These results indicate that appropriate concentrations of ScCl_(3)can improve osteogenic differentiation in the lineage commitment of rBMSCs,and thus,promote bone remodeling.This study implies that ScCl_(3) possesses great potentials in the treatment of bone diseases and would provide new strategy of designing composites by SiCl3 doping for biomedical applications in the future.展开更多
Magnetism has revolutionized important technologies,and continues to bring forth new phenomena in emergent materials and reduced dimensions.Here,using first-principles calculations,we demonstrate that the already-synt...Magnetism has revolutionized important technologies,and continues to bring forth new phenomena in emergent materials and reduced dimensions.Here,using first-principles calculations,we demonstrate that the already-synthesized two-dimensional(2D)Ni-tetracyanoquinodimethane(Ni_(2)(TCNQ)_(2))lattice is a stable ferromagnetism material with multiple spin-polarized Dirac cones.The conical bands in proximity of the Fermi level can be tuned by external tensile strain and show the fourfold degenerate electronic states at the critical tensile strain of~2.35%,whose energy dispersion is consistent with 2D Cairo pentagonal lattice.In addition,spin-orbital coupling can open a band gap at the Dirac point of A,leading to topologically nontrivial electronic states characterized by the non-zero Chern number and the edge states of nanoribbon.Our results offer versatile platforms for the realization of massless spintronics with full-spin polarization in 2D Cairo pentagonal Ni_(2)(TCNQ)_(2) Lattice.展开更多
基金supported by the National Natural Science Foundation of China(51972148,52272212,and 11904131)Shandong Provincial Natural Science Foundation(ZR2020KE056 and ZR2021YQ04)+1 种基金the Major Scientific and Technological Innovation Project of Shandong Province(2021CXGC010603)the Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong。
文摘神经退行性疾病是人类最难治疗的疾病之一.易获取的间充质干细胞(MSCs)可用于自体干细胞移植,间充质干细胞治疗被认为是最有希望的治疗选择之一.然而,传统的生长诱导因子难以实现间充质干细胞的神经分化.电刺激方式可以诱导间充质干细胞的神经分化,但外部电线和复杂的设备给临床治疗带来了很大阻碍.在这项研究中,基于磁电感应效应,我们发现在无需任何生物或化学因子的辅助下,旋转磁场(RMF)驱动下还原氧化石墨烯膜(rGO-M)上产生的无线电信号可以诱导间充质干细胞的神经元样分化.体外实验结果显示,RMF以400 r min^(-1)的速度刺激rGO-M,每天刺激15 min,rGO-M上的MSCs能表达神经元特异性基因和蛋白,连续处理15天后,基因和蛋白的表达量得到明显提升.大鼠体内实验证实,rGO-M上的外源性间充质干细胞可以在旋转磁场的驱动下分化成神经元样细胞.鉴于rGO-M和自体间充质干细胞来源成本较低,rGO-M介导的无线电刺激方法为神经退行性疾病的干细胞治疗提供了一个可行的方案.
基金Project supported by the National Natural Science Foundation of China(51972148,51802115,11904131,21501090)the Project of"20 items of University"of Jinan(2018GXRC031)the Doctoral Fund of University of Jinan(XBS1609)。
文摘Mesenchymal stem cells(MSCs)are multi-potent cells that are able to differentiate and mature into various types of cells under a certain microenvironment for cell therapy and tissue regeneration.Scandium(Sc),an important rare earth element,recently has been intensively investigated in biomedical fields as well as industrial engineering,and chloride channels have been proven to be able to affect osteogenic differentiation.Thus,it is significant to investigate effects of ScCl_(3)on cell activities of MSCs.In this paper,rat bone MSCs(rBMSCs)were co-cultured with various concentrations of ScCl_(3)(1×10^(-8),1×10^(-6),and 1×10^(-4)mol/L)to evaluate their influence on cell proliferation as well as osteogenic and adipogenic differentiation in vitro.The results indicate that ScCl_(3)promotes the proliferation of rBMSCs initially,which is yet reduced upon ion accumulation.We used immunofluorescence staining,quantitative real time polymerase chain reactions,and assays measuring alkaline phosphatase activity,mineralized deposits,and intracytoplasmic lipids to reveal that rBMSCs treated with ScCl_(3)at concentrations of 1×10^(-8)-1×10^(-6)mol/L can enhance levels of osteogenic differentiation in a dosedependent manner and reduce adipogenic differentiation to a certain degree through Wnt/β-catenin signaling pathway.These results indicate that appropriate concentrations of ScCl_(3)can improve osteogenic differentiation in the lineage commitment of rBMSCs,and thus,promote bone remodeling.This study implies that ScCl_(3) possesses great potentials in the treatment of bone diseases and would provide new strategy of designing composites by SiCl3 doping for biomedical applications in the future.
基金A.W.greatly appreciates the National Natural Science Foundation of China(No.11904131)the Natural Science Foundation of Shandong Province(No.ZR2019BA006)+2 种基金M.Z.thanks the supports from the National Natural Science Foundation of China(No.11774201)the Taishan scholarship of Shandong Province.N.R.appreciates the National Natural Science Foundation of China(No.51972148)L.D.thanks the support from the National Natural Science Foundation of China(No.51802118).
文摘Magnetism has revolutionized important technologies,and continues to bring forth new phenomena in emergent materials and reduced dimensions.Here,using first-principles calculations,we demonstrate that the already-synthesized two-dimensional(2D)Ni-tetracyanoquinodimethane(Ni_(2)(TCNQ)_(2))lattice is a stable ferromagnetism material with multiple spin-polarized Dirac cones.The conical bands in proximity of the Fermi level can be tuned by external tensile strain and show the fourfold degenerate electronic states at the critical tensile strain of~2.35%,whose energy dispersion is consistent with 2D Cairo pentagonal lattice.In addition,spin-orbital coupling can open a band gap at the Dirac point of A,leading to topologically nontrivial electronic states characterized by the non-zero Chern number and the edge states of nanoribbon.Our results offer versatile platforms for the realization of massless spintronics with full-spin polarization in 2D Cairo pentagonal Ni_(2)(TCNQ)_(2) Lattice.
