This study investigated bacteria that are associated with milt in cultured samples of African catfish-Clarias gariepinus.Male broodstocks of Clarias gariepinus were collected from private Fish Farm in Ogun State,Niger...This study investigated bacteria that are associated with milt in cultured samples of African catfish-Clarias gariepinus.Male broodstocks of Clarias gariepinus were collected from private Fish Farm in Ogun State,Nigeria,while the fish’s milt was collected and analyzed for microbial load at laboratory of the Department of Microbiology,Federal University of Agriculture,Abeokuta,Ogun State.The molecular characterization of the amplicon was done at International Institute of Tropical Agriculture,Ibadan.Bio-edit was used for importing and mining nucleotide sequences into gene bank.As revealed by the results,bacterial organisms that were present in the milt of Clarias gariepinus are Aeromonas caviae,Proteus mirabilis,Serratia rubidaea,Pseudomonas mosselii,Acinetobacter soli and Klebsiella variicola.The Basic Local Alignment Search Tools revealed the percentage similarity ranging from 86%-97.04%and their accession numbers.These bacteria indicated high levels of faecal contamination in the environment.In conclusion,bacteria were found in the milt of cultured catfish and are capable of being pathogenic to humans and may increase the vertical transfer to fry during breeding and rearing stage.Therefore,fish farmers should maintain a hygiene and serene environment during breeding and culturing of catfish.展开更多
文摘This study investigated bacteria that are associated with milt in cultured samples of African catfish-Clarias gariepinus.Male broodstocks of Clarias gariepinus were collected from private Fish Farm in Ogun State,Nigeria,while the fish’s milt was collected and analyzed for microbial load at laboratory of the Department of Microbiology,Federal University of Agriculture,Abeokuta,Ogun State.The molecular characterization of the amplicon was done at International Institute of Tropical Agriculture,Ibadan.Bio-edit was used for importing and mining nucleotide sequences into gene bank.As revealed by the results,bacterial organisms that were present in the milt of Clarias gariepinus are Aeromonas caviae,Proteus mirabilis,Serratia rubidaea,Pseudomonas mosselii,Acinetobacter soli and Klebsiella variicola.The Basic Local Alignment Search Tools revealed the percentage similarity ranging from 86%-97.04%and their accession numbers.These bacteria indicated high levels of faecal contamination in the environment.In conclusion,bacteria were found in the milt of cultured catfish and are capable of being pathogenic to humans and may increase the vertical transfer to fry during breeding and rearing stage.Therefore,fish farmers should maintain a hygiene and serene environment during breeding and culturing of catfish.
