Soil seed banks are a vital part of ecosystems and influence community dynamics and regeneration.Although soil seed banks in different habitats have been reported, how soil seed banks vary with elevational gradients i...Soil seed banks are a vital part of ecosystems and influence community dynamics and regeneration.Although soil seed banks in different habitats have been reported, how soil seed banks vary with elevational gradients in different climatic zones is still unknown. This paper investigates seed density,species composition and nonconstituent species of forest soil seed banks in Yunnan Province, southwest China. Similarity between the soil seed bank and standing vegetation was also examined. We collected soil samples from sites spanning 12 elevations in tropical rain forests, subtropical evergreen broadleaved forests and subalpine coniferous forests, and transported them to a glasshouse for germination trials for species identification. The soil seed banks of tropical and subtropical forests had much higher seed densities and species richness than those of subalpine forests. Seeds of woody species dominated the soil seed banks of tropical and subtropical forests, while herbs dominated those of subalpine forests.The nonconstituent species in the soil seed banks were all herbs and were most abundant in tropical forests, followed by subtropical forests but were completely absent from subalpine forests.展开更多
Elevational patterns of tree diversity are well studied worldwide.However,few studies have examined how seedlings respond to elevational gradients and whether their responses vary across climatic zones.In this study,w...Elevational patterns of tree diversity are well studied worldwide.However,few studies have examined how seedlings respond to elevational gradients and whether their responses vary across climatic zones.In this study,we established three elevational transects in tropical,subtropical and subalpine mountain forests in Yunnan Province,southern China,to examine the responses of tree species and their seedlings to elevational gradients.Within each transect,we calculated species diversity indices and composition of both adult trees and seedlings at different elevations.For both adult trees and seedlings,we found that species diversity decreased with increasing elevation in both tropical and subalpine transects.Species composition showed significant elevational separation within all three transects.Many species had specific elevational preferences,but abundant tree species that occurred at specific elevations tended to have very limited recruitment in the understory.Our results highlight that the major factors that determine elevational distributions of tree species vary across climatic zones.Specifically,we found that the contribution of air temperature to tree species composition increased from tropical to subalpine transects,whereas the contribution of soil moisture decreased across these transects.展开更多
Background: Caries detection in school oral examinations insufficient accuracy. Objective: To evaluate advantages of introducing quantitative light-induced fluorescence-digital (QLF-D) in school oral examinations. Met...Background: Caries detection in school oral examinations insufficient accuracy. Objective: To evaluate advantages of introducing quantitative light-induced fluorescence-digital (QLF-D) in school oral examinations. Methods: Experiment No. 1. Early demineralized lesions in the upper and lower incisors and canines were visually inspected by three dentists and by QLF-D. The numbers of tooth planes with early demineralized lesions were compared between the methods. Experiment No. 2. Approximal demineralized lesions in molars were assessed by visual inspection, x-ray imaging, and QLF-D. The numbers of tooth planes with demineralized lesions were compared among the methods. Experiment No. 3. Plaque distribution was evaluated by QLF-D and a traditional staining method. The ratio of the diameter of plaque to tooth crown in the tooth axis direction in each method was calculated. The results were evaluated by Pearson’s correlation coefficient analysis and Bland-Altman plot. Results: Experiment No. 1. The three dentists found 0.67 tooth planes on average. QLF-D found 22 tooth planes with early demineralized lesions in the same samples. Experiment No. 2. Fourteen approximal tooth planes of molars were found to have demineralized lesions by x-ray imaging. QLF-D detected 71.4% of the tooth planes out of the 14, whereas visual inspection found 7.1%. Experiment No. 3. The Pearson’s correlation coefficient for the evaluations of plaque distribution between the QLF-D and traditional staining methods was 0.77 (P Conclusion: The results support introduction of QLF-D for use in school dental examinations.展开更多
Aims Quantifying the relative importance of the mechanisms that drive community assembly in forests is a crucial issue in community ecol-ogy.The present study aims to understand the ways in which niche-based and spati...Aims Quantifying the relative importance of the mechanisms that drive community assembly in forests is a crucial issue in community ecol-ogy.The present study aims to understand the ways in which niche-based and spatially based processes influence community assembly in areas in different climatic conditions and how these processes change during the transition from seedling to adult.Methods In this study,we investigated how taxonomic and phylogenetic beta diversity in seedling and adult stages of forest trees change across three elevational transects in tropical,subtropical and subalpine for-ests in Southwest China,and the relationships of these changes to the environment and inter-site distances.We quantified the relative contribution of environmental conditions and spatial distribution to taxonomic and phylogenetic beta diversity of both seedling and adult life stages along each elevational transect.We also quantified the taxonomic and phylogenetic similarity between seedlings and adult trees along elevations.Important Findings Taxonomic and phylogenetic beta diversity of both seedlings and adult trees increased with an increase in both environmental distance and spatial distance in all three transects.On both taxonomic and phylo-genetic levels,the effects of environmental filtering and spatial dispos-ition varied between life stages and among forest types.Phylogenetic similarity between seedlings and adult trees increased with elevation,although the taxonomic similarity did not show clear elevational pat-terns.Our results suggest that the relative contribution of niche-based and space-based processes to taxonomic and phylogenetic assem-blages varies across major plant life stages and among forest types.Our findings also highlight the importance of ontogenetic stages for fully understanding community assembly of long-lived tree species.展开更多
Caterpillars(Lepidoptera and Symphyta larvae)employ diverse visual defensive tactics,and effectiveness of such tactics may be highly dynamic across time due to seasonal changes in the predator assemblages and their pr...Caterpillars(Lepidoptera and Symphyta larvae)employ diverse visual defensive tactics,and effectiveness of such tactics may be highly dynamic across time due to seasonal changes in the predator assemblages and their preferences.However,this has rarely been studied especially in tropical regions.Here we assessed temporal changes in the defensive value of caterpillar color and shape,using six types of plasticine dummy caterpillars:three colors(green,black,and white)x two shapes(curled and straight).These dummy caterpillars were deployed five times over different seasons in tropical forests of Xishuangbanna(China)and,as a comparison,twice in a temperate forest of Hirosaki(Japan).The colors and shapes of dummy caterpillars simulate visual traits of black sawfly larvae which take the curled resting posture in tropical rainforests of Xishuangbanna,apparently masquerading excrements commonly found on plants,while in Hirosaki there is no black-curled sawfly larvae and few excrements on plants.Wc found no significant effects of caterpillar colors or shapes on predation in Hirosaki.In contrast,black and curled caterpillars received significantly lower predation by birds in Xishuangbanna constantly across time.However,we were unable to provide evidence that the black-curled sawfly larvae are masquerading as excrements.Shapes of the dummy caterpillars also affected the predation by ants and parasitoid wasps at certain times.This is the first report on ecological function of the curled posture of sawfly larvae,and we demonstrated the importance to assess the temporal dynamics of predation and effectiveness of defensive tactics in tropical forests.展开更多
基金supported by the National Key Basic Research Program of China (2014CB954100)Yunnan Provincial Foundation of Science and Technology (2014GA003)the QueenslandChinese Academy of Sciences Biotechnology Fund(GJHZ1130)
文摘Soil seed banks are a vital part of ecosystems and influence community dynamics and regeneration.Although soil seed banks in different habitats have been reported, how soil seed banks vary with elevational gradients in different climatic zones is still unknown. This paper investigates seed density,species composition and nonconstituent species of forest soil seed banks in Yunnan Province, southwest China. Similarity between the soil seed bank and standing vegetation was also examined. We collected soil samples from sites spanning 12 elevations in tropical rain forests, subtropical evergreen broadleaved forests and subalpine coniferous forests, and transported them to a glasshouse for germination trials for species identification. The soil seed banks of tropical and subtropical forests had much higher seed densities and species richness than those of subalpine forests. Seeds of woody species dominated the soil seed banks of tropical and subtropical forests, while herbs dominated those of subalpine forests.The nonconstituent species in the soil seed banks were all herbs and were most abundant in tropical forests, followed by subtropical forests but were completely absent from subalpine forests.
基金supported by the National Natural Science Foundation of China(31800353 and 32061123003)the West Light Foundation of the Chinese Academy of Sciences,the Applied Fundamental Research Foundation of Yunnan Province(2019FB038,2014GA003 and 2013FB079)the National Key Basic Research Program of China(2014CB954100)and the QueenslandeChinese Academy of Sciences Biotechnology Fund(GJHZ1130).
文摘Elevational patterns of tree diversity are well studied worldwide.However,few studies have examined how seedlings respond to elevational gradients and whether their responses vary across climatic zones.In this study,we established three elevational transects in tropical,subtropical and subalpine mountain forests in Yunnan Province,southern China,to examine the responses of tree species and their seedlings to elevational gradients.Within each transect,we calculated species diversity indices and composition of both adult trees and seedlings at different elevations.For both adult trees and seedlings,we found that species diversity decreased with increasing elevation in both tropical and subalpine transects.Species composition showed significant elevational separation within all three transects.Many species had specific elevational preferences,but abundant tree species that occurred at specific elevations tended to have very limited recruitment in the understory.Our results highlight that the major factors that determine elevational distributions of tree species vary across climatic zones.Specifically,we found that the contribution of air temperature to tree species composition increased from tropical to subalpine transects,whereas the contribution of soil moisture decreased across these transects.
文摘Background: Caries detection in school oral examinations insufficient accuracy. Objective: To evaluate advantages of introducing quantitative light-induced fluorescence-digital (QLF-D) in school oral examinations. Methods: Experiment No. 1. Early demineralized lesions in the upper and lower incisors and canines were visually inspected by three dentists and by QLF-D. The numbers of tooth planes with early demineralized lesions were compared between the methods. Experiment No. 2. Approximal demineralized lesions in molars were assessed by visual inspection, x-ray imaging, and QLF-D. The numbers of tooth planes with demineralized lesions were compared among the methods. Experiment No. 3. Plaque distribution was evaluated by QLF-D and a traditional staining method. The ratio of the diameter of plaque to tooth crown in the tooth axis direction in each method was calculated. The results were evaluated by Pearson’s correlation coefficient analysis and Bland-Altman plot. Results: Experiment No. 1. The three dentists found 0.67 tooth planes on average. QLF-D found 22 tooth planes with early demineralized lesions in the same samples. Experiment No. 2. Fourteen approximal tooth planes of molars were found to have demineralized lesions by x-ray imaging. QLF-D detected 71.4% of the tooth planes out of the 14, whereas visual inspection found 7.1%. Experiment No. 3. The Pearson’s correlation coefficient for the evaluations of plaque distribution between the QLF-D and traditional staining methods was 0.77 (P Conclusion: The results support introduction of QLF-D for use in school dental examinations.
基金supported by the University of the Chinese Academy of Sciences (UCAS)MSc scholarship (2015-2018)CAS-TWAS President's Fellowship for International Doctoral Students (2018-2022)+5 种基金Chinese Academy of Sciences President's International Fellowship Initiative (2020FYB0003)Strategic Priority Research Program of Chinese Academy of Sciences (XDB31000000)High-End Foreign Experts Program of the High-Level Talent Recruitment Plan of Yunnan Provincethe National Natural Science Foundation of China International (Regional)Cooperation and Exchange Project (32161160324)ZiHui (Wisdom)Yunnan Program (202203AM140026)Xishuangbanna State Rainforest Talent Support Program.
基金This research was supported by the National Natural Science Foundation of China(31400362 and 31670442)National Key Basic Research Program of China(2014CB954100)+2 种基金the West Light Foundation of the Chinese Academy of Sciences,the Chinese Academy of Sciences Youth Innovation Promotion Association(2016352)the Queensland-Chinese Academy of Sciences Biotechnology Fund(GJHZ1130)the Applied Fundamental Research Foundation of Yunnan Province(2014GA003 and 2013FB079).
文摘Aims Quantifying the relative importance of the mechanisms that drive community assembly in forests is a crucial issue in community ecol-ogy.The present study aims to understand the ways in which niche-based and spatially based processes influence community assembly in areas in different climatic conditions and how these processes change during the transition from seedling to adult.Methods In this study,we investigated how taxonomic and phylogenetic beta diversity in seedling and adult stages of forest trees change across three elevational transects in tropical,subtropical and subalpine for-ests in Southwest China,and the relationships of these changes to the environment and inter-site distances.We quantified the relative contribution of environmental conditions and spatial distribution to taxonomic and phylogenetic beta diversity of both seedling and adult life stages along each elevational transect.We also quantified the taxonomic and phylogenetic similarity between seedlings and adult trees along elevations.Important Findings Taxonomic and phylogenetic beta diversity of both seedlings and adult trees increased with an increase in both environmental distance and spatial distance in all three transects.On both taxonomic and phylo-genetic levels,the effects of environmental filtering and spatial dispos-ition varied between life stages and among forest types.Phylogenetic similarity between seedlings and adult trees increased with elevation,although the taxonomic similarity did not show clear elevational pat-terns.Our results suggest that the relative contribution of niche-based and space-based processes to taxonomic and phylogenetic assem-blages varies across major plant life stages and among forest types.Our findings also highlight the importance of ontogenetic stages for fully understanding community assembly of long-lived tree species.
基金supported by Yunnan postdoctoral research project,Yunnan postdoctoral orientation training project,Strategic Priority Research Program of Chinese Academy of Sciences(CAS)(Grant No.XDB31000000)the CAS 135 Programs(2017XTBG-T01 and 2017XTBG-F01)+2 种基金National Natural Science Foundation of China General Program(31770472Shirakami Research Center for Environmental Sciences,Hirosaki Universitysupported by the CAS President's International Fellowship Initiative(2018PC0031)。
文摘Caterpillars(Lepidoptera and Symphyta larvae)employ diverse visual defensive tactics,and effectiveness of such tactics may be highly dynamic across time due to seasonal changes in the predator assemblages and their preferences.However,this has rarely been studied especially in tropical regions.Here we assessed temporal changes in the defensive value of caterpillar color and shape,using six types of plasticine dummy caterpillars:three colors(green,black,and white)x two shapes(curled and straight).These dummy caterpillars were deployed five times over different seasons in tropical forests of Xishuangbanna(China)and,as a comparison,twice in a temperate forest of Hirosaki(Japan).The colors and shapes of dummy caterpillars simulate visual traits of black sawfly larvae which take the curled resting posture in tropical rainforests of Xishuangbanna,apparently masquerading excrements commonly found on plants,while in Hirosaki there is no black-curled sawfly larvae and few excrements on plants.Wc found no significant effects of caterpillar colors or shapes on predation in Hirosaki.In contrast,black and curled caterpillars received significantly lower predation by birds in Xishuangbanna constantly across time.However,we were unable to provide evidence that the black-curled sawfly larvae are masquerading as excrements.Shapes of the dummy caterpillars also affected the predation by ants and parasitoid wasps at certain times.This is the first report on ecological function of the curled posture of sawfly larvae,and we demonstrated the importance to assess the temporal dynamics of predation and effectiveness of defensive tactics in tropical forests.