Immunomodulatory activity of a lupane triterpenoid ester isolated from the eastern Nigeria mistletoe,Loranthus micranthus(Linn)

Objective:To provide further evidence for the ethnomedicinal use of the Eastern Nigeria mistletoe,Loranthus micranthus(L.micranthus),as an immunostimulant.Methods:Solvent fractions from the crude extract of the mistletoe plant was obtained and screened by the cell mediated delayed type hypersensitivity reaction(DTHR) model in mice.Then the immunomodulatory potentials of a major lupane triterpenoid ester isolated from an active hexane fraction of the Eastern Nigeria mistletoe was investigated.Three lupeol-based triterpenoid esters: 7β15α-dihydroxyl-lup-20(29)-ene-3β-palmitate(Ⅰ),7β,15α-dihydroxyl-lup-20(29)- ene-3β-stearate(Ⅱ) and 7β,15α-dihydroxyl-lup-20(29)-ene-3β-decadecanoate(Ⅲ) were isolated from the plant leaves epiphyting on a local kola nut tree and were characterized. Compound 1 was subjected to cell proliferation studies using C57H1/6 splenocvtes at three dose levels(5.25 and 100μg/mL) in presence of controls.Furthermore,the effect of this compound on IL-8 receptor expression was evaluated at three doses(1,5 and 10μg/mL) using the real time polymerase chain reaction assay.Results:This triterpenoid ester produced some enhancement of the splenocytes at the tested doses but at doses higher than 5μg/mL caused inhibition of the IL-8 receptor expression.Conclusions:The present findings support the ethnomedicinal use of the Eastern Nigeria Mistletoe in the management of diseases affecting the immune system.The trilerpenoid(s) have some immunomodulatory abilities on splenocytes and IL-8 receptors and may partly account lor the overall immunomodulatory activity of this plant.

Measuring and evaluating of road roughness conditions with a compact road profiler and ArcGIS

With a wide range of requirements throughout the world, high-quality road management is subject to increasing demand from a perspective of customer-oriented levels of service. In recent years, road administrators are requested to create a visual map of a road network to monitor conditions. To fulfill these requirements, this study conducted as follows. Firstly, this paper introduces a new compact road profiler to collect the profile data at ease. Using the international roughness index （IRI） to assess public roads in local cities of Japan＇s Hokkaido prefecture, this study also provides real-time monitoring of pavement roughness conditions. Moreover, this study deals with an effective method for visualizing collected IRI data as an attribute in a geographic information system （GIS） and the database of Japan digital road map （DRM）. Secondly, the authors present the measurement results of IRI at two different cities during different seasons by using GIS to compare the road conditions. According to the results clarified on different statistical characteristics of road profiles, this study recommends that it is necessary to establish pavement management system （PMS） in consideration of road class, network of local city, and evaluation and management of road conditions in winter quantitatively. Finally, the authors measure and evaluate ride quality by assessing differences between the inner wheel path （IWP） and outer wheel path （OWP） of the vehicle into account, using the previously mentioned profilers and the driving simulator, which is called KITDS. Results show that information from both wheel paths contributes to improve current monitoring process regarding pavement surface, and expects to construct a high level of PMS for road administration in the future.