Dear Editor,We read with interest your recent article on the impact of combined oral contraceptives on ocular tissues.We report a case of a branch retinal vein occlusion（BRVO）associated with use of an etonogestrel/e...Dear Editor,We read with interest your recent article on the impact of combined oral contraceptives on ocular tissues.We report a case of a branch retinal vein occlusion（BRVO）associated with use of an etonogestrel/ethinyl estradiol vaginal ring（NuvaR ing）,an implantable combined hormonal contraceptive,in a young healthy female with no other identifiable risk factors.展开更多
AIM:To evaluate differences in microparticle profiles in vitreous samples between diabetic and non-diabetic eyes undergoing vitrectomy.METHODS:Un-masked cross-sectional series of 34 eyes undergoing vitrectomy.Vitreous...AIM:To evaluate differences in microparticle profiles in vitreous samples between diabetic and non-diabetic eyes undergoing vitrectomy.METHODS:Un-masked cross-sectional series of 34 eyes undergoing vitrectomy.Vitreous specimens were collected and processed to evaluate for membrane integrity(DAPI),apoptosis(Annexin-V),and endothelial-cell origin(V-Cadherin).A BD LSR II flow cytometer was used for analysis and standardized sub-micron-sized beads were used for size comparison.RESULTS:Thirty-four specimens underwent analysis.Greater levels of Annexin-V were found on microparticles from specimens in which blood had entered the vitreous(n=12)compared to those without blood(n=22;52.3%±30.7%vs 19.6%±27.2%,P=0.002).Patients with diabetes having surgery with hemorrhage(n=7)had greater expression of Annexin-V than those without hemorrhage(n=8;62.1%±31.7%vs 18.9%±20.9%,P=0.009).However,in patients with non-diabetic vitreous hemorrhage,the level of Annexin-V expression was not significantly different compared to other disease processes(38.6%±25.7%,n=5 vs 20.0%±30.9%,n=14,P=0.087).CONCLUSION:Increased expression of the apoptotic marker,Annexin-V is detected on vitreous microparticles in diabetes-related vitreous hemorrhage.When evaluating vitreous hemorrhage in patients without diabetes,the apoptotic signal is not significantly different.Vitrectomy in patients with diabetes,and improvement in visual outcomes,may be related to the removal of a serumderived,pro-apoptotic vitreous.Further investigation is warranted in order to identify the molecular characteristics of microparticles that regulate disease.展开更多
文摘Dear Editor,We read with interest your recent article on the impact of combined oral contraceptives on ocular tissues.We report a case of a branch retinal vein occlusion（BRVO）associated with use of an etonogestrel/ethinyl estradiol vaginal ring（NuvaR ing）,an implantable combined hormonal contraceptive,in a young healthy female with no other identifiable risk factors.
基金Supported by an unrestricted grant to Washington University by Research to Prevent Blindness Inc.,New York,NY。
文摘AIM:To evaluate differences in microparticle profiles in vitreous samples between diabetic and non-diabetic eyes undergoing vitrectomy.METHODS:Un-masked cross-sectional series of 34 eyes undergoing vitrectomy.Vitreous specimens were collected and processed to evaluate for membrane integrity(DAPI),apoptosis(Annexin-V),and endothelial-cell origin(V-Cadherin).A BD LSR II flow cytometer was used for analysis and standardized sub-micron-sized beads were used for size comparison.RESULTS:Thirty-four specimens underwent analysis.Greater levels of Annexin-V were found on microparticles from specimens in which blood had entered the vitreous(n=12)compared to those without blood(n=22;52.3%±30.7%vs 19.6%±27.2%,P=0.002).Patients with diabetes having surgery with hemorrhage(n=7)had greater expression of Annexin-V than those without hemorrhage(n=8;62.1%±31.7%vs 18.9%±20.9%,P=0.009).However,in patients with non-diabetic vitreous hemorrhage,the level of Annexin-V expression was not significantly different compared to other disease processes(38.6%±25.7%,n=5 vs 20.0%±30.9%,n=14,P=0.087).CONCLUSION:Increased expression of the apoptotic marker,Annexin-V is detected on vitreous microparticles in diabetes-related vitreous hemorrhage.When evaluating vitreous hemorrhage in patients without diabetes,the apoptotic signal is not significantly different.Vitrectomy in patients with diabetes,and improvement in visual outcomes,may be related to the removal of a serumderived,pro-apoptotic vitreous.Further investigation is warranted in order to identify the molecular characteristics of microparticles that regulate disease.
