BACKGROUND Colorectal cancer(CRC)is the third leading cause of cancer-related deaths in the world.Tumor removal remains the preferred frontline treatment;however,effective non-surgical interventions remain a high prio...BACKGROUND Colorectal cancer(CRC)is the third leading cause of cancer-related deaths in the world.Tumor removal remains the preferred frontline treatment;however,effective non-surgical interventions remain a high priority.5-fluorouracil(5-FU)is a widely used chemotherapy agent,and molecular hydrogen(H2)has been recognized for its antioxidant and anti-inflammatory effects,with research also suggesting its potential anti-tumor effects.Therefore,H2 dissolved in water[hydrogen-rich water(HRW)],with or without 5-FU,may present itself as a novel therapeutic for CRC.AIM To investigate the effects of HRW,with or without 5-FU,as a novel therapeutic for CRC.METHODS CRC was induced in the left flank of inbred Balb/c mice.A total of 24 mice bearing tumors were randomly divided into four groups(n=6 per group)and treated as follows:(1)Control group;(2)5-FU group that received intraperitoneal injection of 5-FU(5 mg/kg)every other day;(3)H2 group that received HRW,created and delivered via dissolving the H2-generating tablet in the animals’drinking water,with 200μL also delivered by oral gavage;and(4)The combination group,H2(administered in same way as for group three)combined with 5-FU administered same way as group two.RESULTS Administration of HRW+5-FU significantly improved tumor weight,tumor size,collagen content and fibrosis as compared to the CRC control group.Specifically,HRW attenuated oxidative stress(OS)and potentiated antioxidant activity(AA),whereas 5-FU treatment exacerbated OS and blunted AA.The combination of HRW+5-FU significantly reduced tumor weight and size,as well as reduced collagen deposition and the degree of fibrosis,while further increasing OS and decreasing AA compared to administration of 5-FU alone.CONCLUSION Administration of HRW,with or without 5-FU,may serve as a therapeutic for treating CRC.展开更多
文摘BACKGROUND Colorectal cancer(CRC)is the third leading cause of cancer-related deaths in the world.Tumor removal remains the preferred frontline treatment;however,effective non-surgical interventions remain a high priority.5-fluorouracil(5-FU)is a widely used chemotherapy agent,and molecular hydrogen(H2)has been recognized for its antioxidant and anti-inflammatory effects,with research also suggesting its potential anti-tumor effects.Therefore,H2 dissolved in water[hydrogen-rich water(HRW)],with or without 5-FU,may present itself as a novel therapeutic for CRC.AIM To investigate the effects of HRW,with or without 5-FU,as a novel therapeutic for CRC.METHODS CRC was induced in the left flank of inbred Balb/c mice.A total of 24 mice bearing tumors were randomly divided into four groups(n=6 per group)and treated as follows:(1)Control group;(2)5-FU group that received intraperitoneal injection of 5-FU(5 mg/kg)every other day;(3)H2 group that received HRW,created and delivered via dissolving the H2-generating tablet in the animals’drinking water,with 200μL also delivered by oral gavage;and(4)The combination group,H2(administered in same way as for group three)combined with 5-FU administered same way as group two.RESULTS Administration of HRW+5-FU significantly improved tumor weight,tumor size,collagen content and fibrosis as compared to the CRC control group.Specifically,HRW attenuated oxidative stress(OS)and potentiated antioxidant activity(AA),whereas 5-FU treatment exacerbated OS and blunted AA.The combination of HRW+5-FU significantly reduced tumor weight and size,as well as reduced collagen deposition and the degree of fibrosis,while further increasing OS and decreasing AA compared to administration of 5-FU alone.CONCLUSION Administration of HRW,with or without 5-FU,may serve as a therapeutic for treating CRC.
