Cells are known to release different types of vesicles such as small extracellular vesicles(sEVs)and large extracellular vesicles(LEVs).sEVs and LEVs play important roles in intercellular communication,pre-metastatic ...Cells are known to release different types of vesicles such as small extracellular vesicles(sEVs)and large extracellular vesicles(LEVs).sEVs and LEVs play important roles in intercellular communication,pre-metastatic niche formation,and disease progression;both can be detected cell culture media and biological fluids.sEVs and LEVs contain a variety of protein and RNA cargo,and they are believed to impact many biological functions of the recipient cells upon their internalization or binding to cell surface proteins.It has recently been established that standard isolation techniques,such as differential ultracentrifugation,yield a mixed population of EVs.However,density gradient ultracentrifugation has been reported to allow the isolation of sEVs without cellular debris.Here,we describe the most common methods used to isolate sEVs from cell culture medium,mouse and human plasma,and a new technique for isolating sEVs from tissues as well.This article also provides detailed procedures to isolate LEVs.展开更多
文摘Cells are known to release different types of vesicles such as small extracellular vesicles(sEVs)and large extracellular vesicles(LEVs).sEVs and LEVs play important roles in intercellular communication,pre-metastatic niche formation,and disease progression;both can be detected cell culture media and biological fluids.sEVs and LEVs contain a variety of protein and RNA cargo,and they are believed to impact many biological functions of the recipient cells upon their internalization or binding to cell surface proteins.It has recently been established that standard isolation techniques,such as differential ultracentrifugation,yield a mixed population of EVs.However,density gradient ultracentrifugation has been reported to allow the isolation of sEVs without cellular debris.Here,we describe the most common methods used to isolate sEVs from cell culture medium,mouse and human plasma,and a new technique for isolating sEVs from tissues as well.This article also provides detailed procedures to isolate LEVs.
