We have developed a two-photon fluorescence microscope capable of imaging up to 4mm inturbid media with micron resolution.The key feature of this instrument is the innovative de-tector,capable of collecting emission p...We have developed a two-photon fluorescence microscope capable of imaging up to 4mm inturbid media with micron resolution.The key feature of this instrument is the innovative de-tector,capable of collecting emission photons from a wider surface area of the sample thandetectors in traditional two-photon microscopes.This detection scheme is extremely efficient inthe collection of emitted photons scattered by turbid media which allows eight fold increase in theimaging depth when compared with conventional two-photon microscopes.Furthermore,thissystem also has in-depth fluorescence lifetime imaging microscopy(FLiM)imaging capabilitywhich increases image contrast.The detection scheme captures emission light in a transmissionconfiguration,making it extremely efficient for the detection of second harmonic generation(SHG)signals,which is generally forward propagating.Here we present imaging experiments oftissue phantoms and in vivo and ea vivo biological tissue performed with this microscope.展开更多
基金Funding was provided by National Institutes of Health P50 GM076516by the National Center for Research Resources(5P41RR003155-27)the National Instit ute of General Medical Sciences(8 P41GM103540-27).
文摘We have developed a two-photon fluorescence microscope capable of imaging up to 4mm inturbid media with micron resolution.The key feature of this instrument is the innovative de-tector,capable of collecting emission photons from a wider surface area of the sample thandetectors in traditional two-photon microscopes.This detection scheme is extremely efficient inthe collection of emitted photons scattered by turbid media which allows eight fold increase in theimaging depth when compared with conventional two-photon microscopes.Furthermore,thissystem also has in-depth fluorescence lifetime imaging microscopy(FLiM)imaging capabilitywhich increases image contrast.The detection scheme captures emission light in a transmissionconfiguration,making it extremely efficient for the detection of second harmonic generation(SHG)signals,which is generally forward propagating.Here we present imaging experiments oftissue phantoms and in vivo and ea vivo biological tissue performed with this microscope.
