Mycotoxins are toxic secondary metabolites produced by filamentous fungi that are commonly detected as natural contaminants in agricultural commodities worldwide.Mycotoxin exposure can lead to mycotoxicosis in both an...Mycotoxins are toxic secondary metabolites produced by filamentous fungi that are commonly detected as natural contaminants in agricultural commodities worldwide.Mycotoxin exposure can lead to mycotoxicosis in both animals and humans when found in animal feeds and food products,and at lower concentrations can affect animal performance by disrupting nutrient digestion,absorption,metabolism,and animal physiology.Thus,mycotoxin contamination of animal feeds represents a significant issue to the livestock industry and is a health threat to food animals.Since prevention of mycotoxin formation is difficult to undertake to avoid contamination,mitigation strategies are needed.This review explores how the mycotoxins aflatoxins,deoxynivalenol,zearalenone,fumonisins and ochratoxin A impose nutritional and metabolic effects on food animals and summarizes mitigation strategies to reduce the risk of mycotoxicity.展开更多
The consumption of long chain polyunsaturated fatty acids (LC-PUFA) is associated with several human health benefits. Most notable of these LC-PUFA is docosahexaenoic acid (DHA C22:6) whose inclusion is considered ess...The consumption of long chain polyunsaturated fatty acids (LC-PUFA) is associated with several human health benefits. Most notable of these LC-PUFA is docosahexaenoic acid (DHA C22:6) whose inclusion is considered essential for optimum human health. Biofortification of common foods such as eggs with DHA has emerged as a specific approach to increase the intake of DHA in human populations. This can be achieved by supplementing poultry rations with feeds like microalgae or fish oil that are rich in DHA, which results in an increased uptake in the egg. Gas chromatography with flame ionization detection (GC-FID) is the method of choice when analyzing food such as eggs for DHA and other fatty acids. For regulatory studies it is desirable to demonstrate that the method is specifically suitable for the analysis of DHA and fatty acids in eggs. The purpose of this paper is to further extend the scope of the AOAC 996.06 methodology examined in the paper by Dillon et al., and to demonstrate the fitness for purpose of the method by examining specific validation parameters. It is a further objective to investigate the stability of DHA and other fatty acids of short and long timepoints. A validation of the method for the determination of DHA and three other fatty acids in eggs is thus presented.展开更多
Research into long-chain polyunsaturated fatty acids (LC-PUFA), such as docosahexaenoic acid (DHA C22:6 n-3), has shown that their inclusion in the human diet is linked with many health benefits. This has led to an in...Research into long-chain polyunsaturated fatty acids (LC-PUFA), such as docosahexaenoic acid (DHA C22:6 n-3), has shown that their inclusion in the human diet is linked with many health benefits. This has led to an increased interest in the enrichment of certain foodstuffs with DHA by supplementing animal fed with DHA-rich ingredients which can lead to an increased uptake in the meat, milk and eggs animal by-products. The microalgae Aurantiochytrium limacinum has been found to be especially useful in this pursuit. It is subsequently desirable to availably have a simple and robust method for the routine analysis of DHA and other fatty acids in the algal biomass. The AOAC method 996.06 is often followed for the analysis of fatty acids in foods and demonstrating that its fitness for purpose in the analysis of DHA and additional fatty acids in Aurantiochytrium limacinum is therefore the objective of this paper. A validation of the method for the determination of DHA and three other fatty acids in Aurantiochytrium limacinum is presented. The method was found to be linear over the following ranges for each fatty acid methyl ester (FAME) analyte;50 to 15,000 μg/ml (C14:0), 300 to 95,000 (C16:0), 25 to 15,000 (C18:0) and 300 to 59,375 (C22:6). The accuracy, precision and LOD and LOQ of the method were confirmed and its robustness tested. The application of the method to assess the stability of Aurantiochytrium limacinum containing two alternative antioxidants was further examined. The investigation showed that DHA was stable over six months with the inclusion of either Duralox? or ethoxyquin as an antioxidant and ethoxyquin could additionally stabilize DHA in Aurantiochytrium limacinum up to 24 months.展开更多
High incidence of traditional and emerging Fusarium mycotoxins in cereal grains and silages can be a potential threat to feed safety and ruminants.Inadequate biodegradation of Fusarium mycotoxins by rumen microflora f...High incidence of traditional and emerging Fusarium mycotoxins in cereal grains and silages can be a potential threat to feed safety and ruminants.Inadequate biodegradation of Fusarium mycotoxins by rumen microflora following ingestion of mycotoxin-contaminated feeds can lead to their circulatory transport to target tissues such as mammary gland.The bovine udder plays a pivotal role in maintaining milk yield and composition,thus,human health.However,toxic effects of Fusarium mycotoxins on bovine mammary gland are rarely studied.In this study,the bovine mammary epithelial cell line was used as an in-vitro model of bovine mammary epithelium to investigate effects of deoxynivalenol(DON),enniatin B(ENB)and beauvericin(BEA)on bovine mammary gland homeostasis.Results indicated that exposure to DON,ENB and BEA for 48 h significantly decreased cell viability in a concentration-dependent manner(P<0.001).Exposure to DON at 0.39μmol/L and BEA at 2.5μmol/L for 48 h also decreased paracellular flux of FITC-40 kDa dextran(P<0.05),whereas none of the mycotoxins affected transepithelial electrical resistance after 48 h exposure.The qPCR was performed for assessment of expression of gene coding tight junction(TJ)proteins,toll-like receptor 4(TLR4)and cytokines after 4,24 and 48 h of exposure.DON,ENB and BEA significantly upregulated the TJ protein zonula occludens-1,whereas markedly downregulated claudin 3(P<0.05).Exposure to DON at 1.35μmol/L for 4 h significantly increased expression of occludin(P<0.01).DON,ENB and BEA significant downregulated TLR4(P<0.05).In contrast,ENB markedly increased expression of cytokines interleukin-6(IL-6)(P<0.001),tumor necrosis factorα(TNF-a)(P<0.05)and transforming growth factor-β(TGF-β)(P<0.01).BEA significantly upregulated IL-6(P<0.001)and TGF-β(P=0.01),but downregulated TNF-α(P<0.001).These results suggest that DON,ENB and BEA can disrupt mammary gland homeostasis by inducing cell death as well as altering its paracellular permeability and expression of genes involved in innate immune function.展开更多
基金funded by Natural Sciences and Engineering Research Council of Canada and Alltech Inc,KY,US[532378-18].
文摘Mycotoxins are toxic secondary metabolites produced by filamentous fungi that are commonly detected as natural contaminants in agricultural commodities worldwide.Mycotoxin exposure can lead to mycotoxicosis in both animals and humans when found in animal feeds and food products,and at lower concentrations can affect animal performance by disrupting nutrient digestion,absorption,metabolism,and animal physiology.Thus,mycotoxin contamination of animal feeds represents a significant issue to the livestock industry and is a health threat to food animals.Since prevention of mycotoxin formation is difficult to undertake to avoid contamination,mitigation strategies are needed.This review explores how the mycotoxins aflatoxins,deoxynivalenol,zearalenone,fumonisins and ochratoxin A impose nutritional and metabolic effects on food animals and summarizes mitigation strategies to reduce the risk of mycotoxicity.
文摘The consumption of long chain polyunsaturated fatty acids (LC-PUFA) is associated with several human health benefits. Most notable of these LC-PUFA is docosahexaenoic acid (DHA C22:6) whose inclusion is considered essential for optimum human health. Biofortification of common foods such as eggs with DHA has emerged as a specific approach to increase the intake of DHA in human populations. This can be achieved by supplementing poultry rations with feeds like microalgae or fish oil that are rich in DHA, which results in an increased uptake in the egg. Gas chromatography with flame ionization detection (GC-FID) is the method of choice when analyzing food such as eggs for DHA and other fatty acids. For regulatory studies it is desirable to demonstrate that the method is specifically suitable for the analysis of DHA and fatty acids in eggs. The purpose of this paper is to further extend the scope of the AOAC 996.06 methodology examined in the paper by Dillon et al., and to demonstrate the fitness for purpose of the method by examining specific validation parameters. It is a further objective to investigate the stability of DHA and other fatty acids of short and long timepoints. A validation of the method for the determination of DHA and three other fatty acids in eggs is thus presented.
文摘Research into long-chain polyunsaturated fatty acids (LC-PUFA), such as docosahexaenoic acid (DHA C22:6 n-3), has shown that their inclusion in the human diet is linked with many health benefits. This has led to an increased interest in the enrichment of certain foodstuffs with DHA by supplementing animal fed with DHA-rich ingredients which can lead to an increased uptake in the meat, milk and eggs animal by-products. The microalgae Aurantiochytrium limacinum has been found to be especially useful in this pursuit. It is subsequently desirable to availably have a simple and robust method for the routine analysis of DHA and other fatty acids in the algal biomass. The AOAC method 996.06 is often followed for the analysis of fatty acids in foods and demonstrating that its fitness for purpose in the analysis of DHA and additional fatty acids in Aurantiochytrium limacinum is therefore the objective of this paper. A validation of the method for the determination of DHA and three other fatty acids in Aurantiochytrium limacinum is presented. The method was found to be linear over the following ranges for each fatty acid methyl ester (FAME) analyte;50 to 15,000 μg/ml (C14:0), 300 to 95,000 (C16:0), 25 to 15,000 (C18:0) and 300 to 59,375 (C22:6). The accuracy, precision and LOD and LOQ of the method were confirmed and its robustness tested. The application of the method to assess the stability of Aurantiochytrium limacinum containing two alternative antioxidants was further examined. The investigation showed that DHA was stable over six months with the inclusion of either Duralox? or ethoxyquin as an antioxidant and ethoxyquin could additionally stabilize DHA in Aurantiochytrium limacinum up to 24 months.
基金The authors acknowledge the financial contributions from the Natural Sciences and Engineering Research Council[401550]Alltech(United States)[054247]to this study.
文摘High incidence of traditional and emerging Fusarium mycotoxins in cereal grains and silages can be a potential threat to feed safety and ruminants.Inadequate biodegradation of Fusarium mycotoxins by rumen microflora following ingestion of mycotoxin-contaminated feeds can lead to their circulatory transport to target tissues such as mammary gland.The bovine udder plays a pivotal role in maintaining milk yield and composition,thus,human health.However,toxic effects of Fusarium mycotoxins on bovine mammary gland are rarely studied.In this study,the bovine mammary epithelial cell line was used as an in-vitro model of bovine mammary epithelium to investigate effects of deoxynivalenol(DON),enniatin B(ENB)and beauvericin(BEA)on bovine mammary gland homeostasis.Results indicated that exposure to DON,ENB and BEA for 48 h significantly decreased cell viability in a concentration-dependent manner(P<0.001).Exposure to DON at 0.39μmol/L and BEA at 2.5μmol/L for 48 h also decreased paracellular flux of FITC-40 kDa dextran(P<0.05),whereas none of the mycotoxins affected transepithelial electrical resistance after 48 h exposure.The qPCR was performed for assessment of expression of gene coding tight junction(TJ)proteins,toll-like receptor 4(TLR4)and cytokines after 4,24 and 48 h of exposure.DON,ENB and BEA significantly upregulated the TJ protein zonula occludens-1,whereas markedly downregulated claudin 3(P<0.05).Exposure to DON at 1.35μmol/L for 4 h significantly increased expression of occludin(P<0.01).DON,ENB and BEA significant downregulated TLR4(P<0.05).In contrast,ENB markedly increased expression of cytokines interleukin-6(IL-6)(P<0.001),tumor necrosis factorα(TNF-a)(P<0.05)and transforming growth factor-β(TGF-β)(P<0.01).BEA significantly upregulated IL-6(P<0.001)and TGF-β(P=0.01),but downregulated TNF-α(P<0.001).These results suggest that DON,ENB and BEA can disrupt mammary gland homeostasis by inducing cell death as well as altering its paracellular permeability and expression of genes involved in innate immune function.