Rabilé is a ferment of dolo, a traditional sorghum beer of Burkina Faso and it is harvested at the end of dolo fermentation process. It is a significant source of proteins and it is used like a condiment and food...Rabilé is a ferment of dolo, a traditional sorghum beer of Burkina Faso and it is harvested at the end of dolo fermentation process. It is a significant source of proteins and it is used like a condiment and food seasoning by some communities. The present study characterized and identified the yeasts isolated from Rabilé. A total of 70 samples were collected from 7 localities of Burkina Faso with dolo sellers according to the ethnic groups. The aerobic mesophilic flora count from Rabilé varied from 8.34 ± 0.72 to 10.07 ± 0.51 log10 CFU·g-1 and yeast varied from 7.24 to 8.28 log10 UFC·g-1. Based on morphological, cultural, sexual and biochemical (carbon and nitrogen assimilation) profiles, 50 yeast strains were identified and found to belong to 4 genera and 7 species. Saccharomyces cerevisiae was found as most predominant yeasts species of yeast in of Rabilé with 64 %, Rhodotorula muciloginosa, (8%), Candida pseudorhangii (6%), Candida heliconiae (12%), Candida utilis (4%), Shizosaccharomyces pombe (4%) and Sporobolomyces odoratus (2%). This activity has been carried out in the Laboratory of Biotechnology for Food and Nutritional Sciences, University Ouaga I Pr Josph KY-ZERBO, Burkina Faso, and it was done from June to October 2018.展开更多
Urban truck farming in developing countries appears of great importance to overcome unemployment and poverty. However, the quality of wastewater used for such activity could expose populations to waterborne diseases. ...Urban truck farming in developing countries appears of great importance to overcome unemployment and poverty. However, the quality of wastewater used for such activity could expose populations to waterborne diseases. The microbial quality of wastewaters used for truck farming in Ouagadougou city, was examined for the presence of bacterial and parasitological fecal indicators during the dry-hot season (May) and the wet season (July) in 2012. The wastewaters of three water reservoirs and two canals intensively used for truck farming were analyzed throughout the study. These indicators were also monitored in waste stabilization ponds during wastewater treatment in 3 plants of the city. For all the selected sampling sites, the concentrations of microbial indicators in water were significantly higher in the dry-hot season compared to the wet one (p - 53,800 CFU/100ml for Escherichia coli, 8200 - 108,400 CFU/100ml for fecal coliforms, 650 - 45,000 CFU/100ml) for fecal streptococcus, and 0 - 2.4 eggs/l for helminthes during the study periods. For wastewater under treatment in waste stabilization ponds, significant microbial concentration drops (p 0.0001) in the range of 82% - 100%, 78% - 98%, 60% - 100% and 82% - 88%, respectively were recorded between the anaerobic and the maturation ponds. The later results highlighted that improving the refining performances of the waste stabilization ponds technology could help decreasing health risks related to wastewater reuse in urban agriculture for a sustainable development of cities in developing countries.展开更多
文摘Rabilé is a ferment of dolo, a traditional sorghum beer of Burkina Faso and it is harvested at the end of dolo fermentation process. It is a significant source of proteins and it is used like a condiment and food seasoning by some communities. The present study characterized and identified the yeasts isolated from Rabilé. A total of 70 samples were collected from 7 localities of Burkina Faso with dolo sellers according to the ethnic groups. The aerobic mesophilic flora count from Rabilé varied from 8.34 ± 0.72 to 10.07 ± 0.51 log10 CFU·g-1 and yeast varied from 7.24 to 8.28 log10 UFC·g-1. Based on morphological, cultural, sexual and biochemical (carbon and nitrogen assimilation) profiles, 50 yeast strains were identified and found to belong to 4 genera and 7 species. Saccharomyces cerevisiae was found as most predominant yeasts species of yeast in of Rabilé with 64 %, Rhodotorula muciloginosa, (8%), Candida pseudorhangii (6%), Candida heliconiae (12%), Candida utilis (4%), Shizosaccharomyces pombe (4%) and Sporobolomyces odoratus (2%). This activity has been carried out in the Laboratory of Biotechnology for Food and Nutritional Sciences, University Ouaga I Pr Josph KY-ZERBO, Burkina Faso, and it was done from June to October 2018.
基金ISP-SUEDE/RA-BIOTECH,PACER-UEMOA/RA-BIO-TECH,National Office of Water and Sanitation(ONEA)and CNRST/IRSS,for financial and technical supports
文摘Urban truck farming in developing countries appears of great importance to overcome unemployment and poverty. However, the quality of wastewater used for such activity could expose populations to waterborne diseases. The microbial quality of wastewaters used for truck farming in Ouagadougou city, was examined for the presence of bacterial and parasitological fecal indicators during the dry-hot season (May) and the wet season (July) in 2012. The wastewaters of three water reservoirs and two canals intensively used for truck farming were analyzed throughout the study. These indicators were also monitored in waste stabilization ponds during wastewater treatment in 3 plants of the city. For all the selected sampling sites, the concentrations of microbial indicators in water were significantly higher in the dry-hot season compared to the wet one (p - 53,800 CFU/100ml for Escherichia coli, 8200 - 108,400 CFU/100ml for fecal coliforms, 650 - 45,000 CFU/100ml) for fecal streptococcus, and 0 - 2.4 eggs/l for helminthes during the study periods. For wastewater under treatment in waste stabilization ponds, significant microbial concentration drops (p 0.0001) in the range of 82% - 100%, 78% - 98%, 60% - 100% and 82% - 88%, respectively were recorded between the anaerobic and the maturation ponds. The later results highlighted that improving the refining performances of the waste stabilization ponds technology could help decreasing health risks related to wastewater reuse in urban agriculture for a sustainable development of cities in developing countries.