Effects of hydroxyl alkaline compounds on dermatophytic cells

Objective:To determine the effect of hydroxide compounds on the viability of dermatophytic cells.Methods:Two strains(Trichophyton mentagrophytes and Epidermophyton floccosum)of dermatophytes were clinical isolated from an old male patient with tinea corporis at AL-Hussein General Hospital of Karbala Province.Skin scales of fungal lesion were cultured on Sabouraud dextrose agar containing 0.05 g chloramphenicol.The hydroxide compounds were dissolved in melted fungal media to obtain different percentages(0.625%,1.25%,2.5%,5%).Then the fungal growth on solid media containing tested compounds was determined by using colony diameter method and different concentrations of KOH,NaOH,Mg(OH)_(2)and Ca(OH)_(2)were tested against two species of dermatophytes.Experiments were repeated triplicate for statistical analysis and the data were analyzed for SE of each experiment.Results:KOH and NaOH(down to 1.25%)showed the ability to completely inhibit the growth of both strains of dermatophytes(Trichophyton mentagrophytes and Epidermophyton floccosum).Other two hydroxide compounds revealed variable effects on dermatophytes cells.Conclusions:The strong hydroxide compounds showed a harmful effect on fungal structures and functions.In addition to diagnosis,pathogenic fungi like dermatophytes were killed after treating with these hydroxide agents.

Lactate dehydrogenase as an indicator of liver,muscular and cancer diseases

Objective:To determine lactate dehydrogenase(LDH)level as an indicator of liver,muscular or cancer diseases in patients of more than 40 years of age.Methods:Ninety-one patients(43 females and 48 males)had been tested for LDH and liver function tests(LFTs).Creatine kinase(CK)levels were measurement only in patients who had high levels of LDH.Results:As an indicator for liver diseases,high levels of LDH and one or more of LFTs,especially alkaline phosphatase,had been observed in 12 patients(8 females and 4 males).For muscular damage,measurement of CK in patients with elevated levels of LDH and normal levels of LFT revealed that CK values elevated in three males and one female.Whereas high LDH levels,as an indicator for cancer diseases,were found in three males and one female who had normal values of LFTs and CK.Conclusions:LDH can be regarded as a good biomarker for diagnosis of liver,muscular and cancer diseases.There is little variable between males and females in the elevated value of LDH.Patients who had high values of LDH,although they had normal levels of LFTs and CK are proposed to have unidentified cancer disease.