This study describes a simple,rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine（6-MAM） as major metabolite of heroin in biological samples.The method is b...This study describes a simple,rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine（6-MAM） as major metabolite of heroin in biological samples.The method is based upon the catalytic effect of 6-MAM on the oxidation of Janus Green by bromate in acid media.The reaction was followed spectrophotometrically by measuring the decrease in absorbance of Janus Green at 618 nm.The dependence of sensitivity on the reaction variables was studied.Under optimum conditions,two linear calibration curves over the range 0.1-1.0μg mL^（-1） and 1.0-34.0μg mL^（-1） of 6- MAM were obtained.The detection limit was 1.2×10~2μg mL^（-1） of 6-MAM.The relative standard deviations for six replicate determinations of 0.8 and 5.0μg mL^（-1） of 6-MAM were 1.4 and 1.1%respectively.The effect of various species commonly associated with heroin in real samples was also investigated.The proposed method was successfully applied in human urine and serum samples with satisfactory results.展开更多
A simple,rapid and sensitive method has been described for the determination of the trace amounts of bromide.The method is based on the catalytic effect of bromide ion on the oxidation of methylene blue by bromate in ...A simple,rapid and sensitive method has been described for the determination of the trace amounts of bromide.The method is based on the catalytic effect of bromide ion on the oxidation of methylene blue by bromate in sulfuric acid media.The reaction is monitored spectrophotometrically by measuring the decrease in the absorbance of methylene blue at 665 nm. Bromide was determined in the range of 0.05-1.90μg/mL with the detection limit of 0.03μg/mL.The relative standard deviations of five replicate determinations of 0.20 and 10.0μg/mL of bromide were 2.4%and 1.8%,respectively.The influence of potential interfering ions and substances was studied.The method is applied to the analysis of bromide in clidinium-c tablet as a real sample.展开更多
文摘This study describes a simple,rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine（6-MAM） as major metabolite of heroin in biological samples.The method is based upon the catalytic effect of 6-MAM on the oxidation of Janus Green by bromate in acid media.The reaction was followed spectrophotometrically by measuring the decrease in absorbance of Janus Green at 618 nm.The dependence of sensitivity on the reaction variables was studied.Under optimum conditions,two linear calibration curves over the range 0.1-1.0μg mL^（-1） and 1.0-34.0μg mL^（-1） of 6- MAM were obtained.The detection limit was 1.2×10~2μg mL^（-1） of 6-MAM.The relative standard deviations for six replicate determinations of 0.8 and 5.0μg mL^（-1） of 6-MAM were 1.4 and 1.1%respectively.The effect of various species commonly associated with heroin in real samples was also investigated.The proposed method was successfully applied in human urine and serum samples with satisfactory results.
文摘A simple,rapid and sensitive method has been described for the determination of the trace amounts of bromide.The method is based on the catalytic effect of bromide ion on the oxidation of methylene blue by bromate in sulfuric acid media.The reaction is monitored spectrophotometrically by measuring the decrease in the absorbance of methylene blue at 665 nm. Bromide was determined in the range of 0.05-1.90μg/mL with the detection limit of 0.03μg/mL.The relative standard deviations of five replicate determinations of 0.20 and 10.0μg/mL of bromide were 2.4%and 1.8%,respectively.The influence of potential interfering ions and substances was studied.The method is applied to the analysis of bromide in clidinium-c tablet as a real sample.
