Photodynamic therapy(PDT)is a novel approach for the anti-tumor treatment of photosensitive drugs and laser activation.In the study,5-aminolevulinic acid(ALA)was used as a photosensitizer to mediate PDT to inhibit the...Photodynamic therapy(PDT)is a novel approach for the anti-tumor treatment of photosensitive drugs and laser activation.In the study,5-aminolevulinic acid(ALA)was used as a photosensitizer to mediate PDT to inhibit the growth,metastasis and epithelial-mesenchymal transformation of melanoma cells.Firstly,the therapeutic effect of PDT was investigated through in vitro experiments.Human melanoma A375 cells cultured with ALA were treated with different light intensity,and the cell survival rates of A375 cells were measured by CCK8 method.Subsequently,the metastasis and invasion rates of A375 cells were detected by scratch test and Transwell test.Western blot was used to detect the expression of molecules related to epithelial mesenchymal transformation,such as E-cadherin,Snail,TGF-β,Smad2 and Smad3.In vitro results showed that A375 cells in the ALA-PDT treatment group showed weaker migration and invasion ability,while A375 cells in the control group showed higher migration and invasion rate(P<0.05).E-cadherin expression was significantly increased in the ALA-PDT treatment group(P<0.01),while expression of Snail,Smad2,Smad3 and TGF-βwere significantly decreased in the ALA-PDT treatment group(P<0.05).Therefore,ALA is a good photosensitizer that can inhibit the migration and invasion of melanoma,which also can inhibit the epithelial mesenchymal transformation of melanoma.展开更多
文摘Photodynamic therapy(PDT)is a novel approach for the anti-tumor treatment of photosensitive drugs and laser activation.In the study,5-aminolevulinic acid(ALA)was used as a photosensitizer to mediate PDT to inhibit the growth,metastasis and epithelial-mesenchymal transformation of melanoma cells.Firstly,the therapeutic effect of PDT was investigated through in vitro experiments.Human melanoma A375 cells cultured with ALA were treated with different light intensity,and the cell survival rates of A375 cells were measured by CCK8 method.Subsequently,the metastasis and invasion rates of A375 cells were detected by scratch test and Transwell test.Western blot was used to detect the expression of molecules related to epithelial mesenchymal transformation,such as E-cadherin,Snail,TGF-β,Smad2 and Smad3.In vitro results showed that A375 cells in the ALA-PDT treatment group showed weaker migration and invasion ability,while A375 cells in the control group showed higher migration and invasion rate(P<0.05).E-cadherin expression was significantly increased in the ALA-PDT treatment group(P<0.01),while expression of Snail,Smad2,Smad3 and TGF-βwere significantly decreased in the ALA-PDT treatment group(P<0.05).Therefore,ALA is a good photosensitizer that can inhibit the migration and invasion of melanoma,which also can inhibit the epithelial mesenchymal transformation of melanoma.
