Objective:To investigate the effect of leaf aqueous extract from Gongronema latifolium(G.latifolium)on CCl_(4)induced-oxidative stress in Wistar rat.This effect was assessed by measuring liver marker enzymes activity,...Objective:To investigate the effect of leaf aqueous extract from Gongronema latifolium(G.latifolium)on CCl_(4)induced-oxidative stress in Wistar rat.This effect was assessed by measuring liver marker enzymes activity,analyzing the antioxidant parameters,lipid profile estimation and lipid peroxidation by-product following CCl_(4)induced-oxidative stress.Methods:Milled G.latifolium leaves were subjected to aqueous extraction and the filtrate was evaporated between 40-60°C under reduced pressure and a calculated volume of the leaf extract was administered at a dose of 500 mg/kg body weight.Thirty-five rats were grouped into seven groups of 5 animals each namely;control and experimental groups.The experimental groups were treated with 2.0 mL/kg body weight CCl_(4),25 mg/kg body weight/day silymarin(a standard hepatoprotective antioxidant),500 mg/kg body weight aqueous extract of G.latifolium leaves were administered to the CCl_(4)treated rats for 21 days.Results:Administration of the extract and silymarin increase significantly(P<0.05)in liver marker enzyme(aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase and gamma-glutamyl transpeptidase)activities in serum,liver and kidney in the treated groups when compared with untreated groups.The antioxidant parameters(catalase,peroxidase and glutathione S-transferase)were significantly elevated(P<0.05)in animals treated with the extract and silymarin in comparison with untreated groups.CCl_(4)induced oxidative stress mediated variations in total cholesterol,triacylglycerides,high-density lipoprotein cholesterol,low density lipoprotein cholesterol and very low-density lipoprotein cholesterol were restored significantly(P<0.05)by the extract.Conclusions:The result obtained from this study indicated the antioxidant and antidyslipidemic potentials of the aqueous extract of G.latifolium leaves.展开更多
文摘Objective:To investigate the effect of leaf aqueous extract from Gongronema latifolium(G.latifolium)on CCl_(4)induced-oxidative stress in Wistar rat.This effect was assessed by measuring liver marker enzymes activity,analyzing the antioxidant parameters,lipid profile estimation and lipid peroxidation by-product following CCl_(4)induced-oxidative stress.Methods:Milled G.latifolium leaves were subjected to aqueous extraction and the filtrate was evaporated between 40-60°C under reduced pressure and a calculated volume of the leaf extract was administered at a dose of 500 mg/kg body weight.Thirty-five rats were grouped into seven groups of 5 animals each namely;control and experimental groups.The experimental groups were treated with 2.0 mL/kg body weight CCl_(4),25 mg/kg body weight/day silymarin(a standard hepatoprotective antioxidant),500 mg/kg body weight aqueous extract of G.latifolium leaves were administered to the CCl_(4)treated rats for 21 days.Results:Administration of the extract and silymarin increase significantly(P<0.05)in liver marker enzyme(aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase and gamma-glutamyl transpeptidase)activities in serum,liver and kidney in the treated groups when compared with untreated groups.The antioxidant parameters(catalase,peroxidase and glutathione S-transferase)were significantly elevated(P<0.05)in animals treated with the extract and silymarin in comparison with untreated groups.CCl_(4)induced oxidative stress mediated variations in total cholesterol,triacylglycerides,high-density lipoprotein cholesterol,low density lipoprotein cholesterol and very low-density lipoprotein cholesterol were restored significantly(P<0.05)by the extract.Conclusions:The result obtained from this study indicated the antioxidant and antidyslipidemic potentials of the aqueous extract of G.latifolium leaves.
