LSTDA: Link Stability and Transmission Delay Aware Routing Mechanism for Flying Ad-Hoc Network (FANET)

The paper presents a new protocol called Link Stability and Transmission Delay Aware(LSTDA)for Flying Adhoc Network(FANET)with a focus on network corridors(NC).FANET consists of Unmanned Aerial Vehicles(UAVs)that face challenges in avoiding transmission loss and delay while ensuring stable communication.The proposed protocol introduces a novel link stability with network corridors priority node selection to check and ensure fair communication in the entire network.The protocol uses a Red-Black(R-B)tree to achieve maximum channel utilization and an advanced relay approach.The paper evaluates LSTDA in terms of End-to-End Delay(E2ED),Packet Delivery Ratio(PDR),Network Lifetime(NLT),and Transmission Loss(TL),and compares it with existing methods such as Link Stability Estimation-based Routing(LEPR),Distributed Priority Tree-based Routing(DPTR),and Delay and Link Stability Aware(DLSA)using MATLAB simulations.The results show that LSTDA outperforms the other protocols,with lower average delay,higher average PDR,longer average NLT,and comparable average TL.

利用SPF鸡胚分离传染性法氏囊病病毒超强毒的研究

传染性法氏囊病病毒(IBDV)超强毒(vvIBDV)不适应鸡胚成纤维细胞培养,其分离鉴定通常采用直接接种无特定病原体(SPF)鸡的方法。然而该方法需要特殊设备,成本较高,较繁琐。研究旨在探讨SPF鸡胚作为IBDV分离工具的可行性,将IBDV阳性病料混悬液通过绒毛尿囊膜途径接种9~11日胚龄SPF鸡胚,并盲传三代成功分离获得vvIBDV(IBD16GX01株)。分离毒各代次序列一致,且与SPF鸡分离氨基酸同源性100%。结果表明,SPF鸡胚可用于vvIBDV的分离鉴定,为vvIBDV分子流行病学研究奠定基础。

Engineering Geological and Geotechnical Investigations for Design of Oxygen Plant

The most important aspect of every civil engineering project is acquiring reliable information on the ground on which the project will be constructed. This research includes a site investigation, which is seen as a primary stage in gathering geological, geotechnical, and other essential engineering data for structures’ safe and cost-effective design. Five boreholes at well-spaced spots were drilled for subsurface investigation at a maximum depth of 15 m to 30 m. The standard penetration tests (SPT) were performed at different depths, soil samples were taken at various intervals, and lithological changes were observed. The friction angle was between 19.6ºand 33.03º, whereas the cohesion ranges between 0.25 kg/cm2 and 0.42 kg/cm2, indicating a strong resistance to shearing and a high capacity to sustain the load. Furthermore, the soil samples’ maximum dry density ranges from 1.63 g/cm3 to 1.80 g/cm3. In addition, water table depths were recorded from 6.0 m to 7.0 m. The net bearing capacity for isolated/pad foundation at a depth of 1.5 m to 2.5 m below the ground level has been calculated as 95.0 to 120.0 kPa and 120.0 to 180.0 kPa for raft foundation. The net allowable pressure settlement limits for isolated/pad and raft foundations are 25 mm (1-inch) and 50 mm (2-inches), respectively. The investigation has found no severe geological flaws on the proposed construction site, and therefore it is appropriate for the construction of an Air Separation Unit (ASU) Oxygen Plant.

三种传染性法氏囊病疫苗对文昌鸡免疫效果的研究

传染性法氏囊病(Infectious bursal disease,IBD)是危害文昌鸡的重要传染病之一。为了筛选最适合文昌鸡的IBD防控措施,试验选用新型重组疫苗(rGt HLJVP2)和商品化的弱毒疫苗、中毒力疫苗,评价其免疫效果。结果显示,重组疫苗(rGt HLJVP2)组表现更高的抗体滴度,且致死性攻毒后保护率为100%,为文昌鸡的疫病科学防控提供了新思路。

Anomaly Based Camera Prioritization in Large Scale Surveillance Networks

Digital surveillance systems are ubiquitous and continuously generate massive amounts of data,and manual monitoring is required in order to recognise human activities in public areas.Intelligent surveillance systems that can automatically identify normal and abnormal activities are highly desirable,as these would allow for efficient monitoring by selecting only those camera feeds in which abnormal activities are occurring.This paper proposes an energy-efficient camera prioritisation framework that intelligently adjusts the priority of cameras in a vast surveillance network using feedback from the activity recognition system.The proposed system addresses the limitations of existing manual monitoring surveillance systems using a three-step framework.In the first step,the salient frames are selected from the online video stream using a frame differencing method.A lightweight 3D convolutional neural network(3DCNN)architecture is applied to extract spatio-temporal features from the salient frames in the second step.Finally,the probabilities predicted by the 3DCNN network and the metadata of the cameras are processed using a linear threshold gate sigmoid mechanism to control the priority of the camera.The proposed system performs well compared to state-of-theart violent activity recognition methods in terms of efficient camera prioritisation in large-scale surveillance networks.Comprehensive experiments and an evaluation of activity recognition and camera prioritisation showed that our approach achieved an accuracy of 98%with an F1-score of 0.97 on the Hockey Fight dataset,and an accuracy of 99%with an F1-score of 0.98 on the Violent Crowd dataset.

The circulation of unique reassortment strains of infectious bursal disease virus in Pakistan

Infectious bursal disease(IBD),caused by IBD virus(IBDV),is one of the most devastating and immunosuppressive diseases of the poultry and has been a constraint on the sustainable poultry production around the globe including Pakistan.While the disease is threatening the poulty industry,the nature of predominant strains of IBDV in Pakistan remained l-defined.In this study,an epidemiology survey was conducted in the main chicken-farming regions of Pakistan.The batch of Pakistan IBDVs genes simultaneously covering both VP1 and VP2 were amplified,sequenced,and analyzed.The unique segment-reassortant IBDVs(vv-A/Uniq-B),carrying segmentA from vvIBDV and segment B from one unique ancestor,were identifed as one important type of circulating strains in Pakistan.The data also discovered the characteristic molecular features of Pakistan IBDVs,which will contribute to scientific vaccine selection and effective prevention of the disease.

DNA Fingerprinting of Essential Commercialized Medicinal Plants from Pakistan

Development of fingerprints based on DNA markers is necessary for proper identification and standardization of plant species. These techniques are widely used to develop an unquestionable method of plant identification to protect the patents and quality control for industry. In this study, fifteen commercially important medicinal plants of Pakistan were collected from botanical garden of Qarshi Industries (Pvt.) Ltd, Pakistan. The objective was to optimize the extraction of genomic DNA for use in a PCR-based random amplified polymorphic DNA marker approach. The initial protocol used 60 decamers to amplify scorable amplicons;only nine markers produced significant bands in genomic DNA of medicinal plants. These markers generated 51 bands ranging between 250 and 1600 bp. The most important property of genomic markers is polymorphism to enable specific identification;all the used markers showed 100% polymorphism across 15 different plants. Further, six decamers amplified specific bands to reliably identify 8 species. The amplified bands were arranged in a binary matrix and analyzed by DNAMAN version 5.2.2 statistical software. A homology tree was constructed using binary data for nine markers, and four major clusters/clades were observed. The Rose, Mentha and Stevia accessions had shown clear clustering and grouped in major clusters/clads I, II and III respectively. Sixty decamers amplified 51 polymorphic loci in the genomes of 15 commercially valuable accessions. Moreover clear phylogenetic construction was observed in the generation of homolog tree. This protocol could therefore be useful to provide a baseline to authenticate, identify and perform phylogenetic analysis of important medicinal plants used in the Pakistani herbal medicine industry.

The Oscillatory Motion of Oldroyd-B Fluid by Incorporating Some of the Mechanical Factors

The aim of this paper is to present a numerical study of oscillatory motion of Oldroyd-B fluid in a uniform magnetic field through a small circular pipe. First, we derive the orientation stress tensor by considering the Brownian force. Then, the orientation stress tensor is incorporated by taking Hookean dumbbells on Brownian configuration fields in the Oldroyd-B model. The Oldroyd-B model is then reformulated coupled with the momentum equation and the total stress tensor. Finally, we analyze the orientation stress tensor in the pipe by the numerical simulations of the model and showed that the effect of orientation stress tensor is considerable although the Brownian force is sufficiently small.

Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan

Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However.