Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the reversible hydration of CO<sub>2</sub> to and H<sup>+</sup>. CA plays a crucial role in CO<sub>2</sub>...Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the reversible hydration of CO<sub>2</sub> to and H<sup>+</sup>. CA plays a crucial role in CO<sub>2</sub> transport, acid-base balance, and in linking local acidosis to O<sub>2</sub> unloading from hemoglobin and also facilitates lactate shuttling across the monocarboxylate transporters (MCT). The study aimed to investigate the influence of hyperglycemia on erythrocyte carbonic anhydrase activity and lactic acid in type II diabetic patients. Method: Red blood cell carbonic anhydrase activity was determined in washed lysed-hemolysate by the action of the enzyme on the substrate p-nitrophenyl acetate. The absorbance of released p-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ion exchange method (Spectrum Diagnostic Kit). Blood glucose, lactate, cholesterol and triglyceride were determined using Accutrend GCT meter (Roche, Germany) with cobias<sup>®</sup> test strips. Results: The present study showed that hyperglycemia significantly (p < 0.05) increased both erythrocyte carbonic anhydrase activity and blood lactate level in type II diabetic patients. Conclusion: We may conclude that hyperglycemia may be responsible for the increased activity of carbonic anhydrase activity and blood lactate concentration.展开更多
文摘Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the reversible hydration of CO<sub>2</sub> to and H<sup>+</sup>. CA plays a crucial role in CO<sub>2</sub> transport, acid-base balance, and in linking local acidosis to O<sub>2</sub> unloading from hemoglobin and also facilitates lactate shuttling across the monocarboxylate transporters (MCT). The study aimed to investigate the influence of hyperglycemia on erythrocyte carbonic anhydrase activity and lactic acid in type II diabetic patients. Method: Red blood cell carbonic anhydrase activity was determined in washed lysed-hemolysate by the action of the enzyme on the substrate p-nitrophenyl acetate. The absorbance of released p-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ion exchange method (Spectrum Diagnostic Kit). Blood glucose, lactate, cholesterol and triglyceride were determined using Accutrend GCT meter (Roche, Germany) with cobias<sup>®</sup> test strips. Results: The present study showed that hyperglycemia significantly (p < 0.05) increased both erythrocyte carbonic anhydrase activity and blood lactate level in type II diabetic patients. Conclusion: We may conclude that hyperglycemia may be responsible for the increased activity of carbonic anhydrase activity and blood lactate concentration.
