Objective: To evaluate the in vitro bioactivity and phytochemical analysis of two marine macro-algae viz., Sargassum wightii (S. wightii) and Gracilaria verrucosa (G. verrucosa). Methods: The marine macro-algae were c...Objective: To evaluate the in vitro bioactivity and phytochemical analysis of two marine macro-algae viz., Sargassum wightii (S. wightii) and Gracilaria verrucosa (G. verrucosa). Methods: The marine macro-algae were collected from Gulf of Mannar (latitude 8°55'–9°15' N and longitude 78°–79°16' E), India. The collected marine macro-algae were shade dried, powdered and extracted in Soxhlet apparatus with acetone, methanol, chloroform and petroleum ether separately. Antimicrobial assay was performed against nine bacterial pathogens (isolated from infected ornamental fish) and two fungal strains. The extracts were further tested for anti-hemolytic activity following standard protocol. The methanol extracts were also tested for its anti-cancer property against human AGS cell line. Results: High antibacterial activity was displayed by the petroleum ether extract of G. verrucosa against Micrococcus sp. ((31.5 ± 6.5) mm)Maximum [(21.5 ± 1.0) mm] anti-fungal activity was exerted by the methanol extracts of S. wightii and G. verrucosa against Candida sp. Acetone extract of G. verrucosa produced better anti-hemolytic activity (96.47% ± 0.31%) than other tested extracts. Methanol extract of both seaweeds failed to produce any anti-cancer activity against human AGS cell line. Quantitative phytochemical analysis revealed elevated levels of alkaloids (56 mg/g), saponins (44.5 mg/g), flavonoids (500 mg/g) and phenols (220 mg/g) in G. verrucosa when compared to S. wightii. Conclusions: The tested macro-algae are good source of bioactive compounds, and G. verrucosa displayed better activity than S. wightii and could be explored further.展开更多
文摘Objective: To evaluate the in vitro bioactivity and phytochemical analysis of two marine macro-algae viz., Sargassum wightii (S. wightii) and Gracilaria verrucosa (G. verrucosa). Methods: The marine macro-algae were collected from Gulf of Mannar (latitude 8°55'–9°15' N and longitude 78°–79°16' E), India. The collected marine macro-algae were shade dried, powdered and extracted in Soxhlet apparatus with acetone, methanol, chloroform and petroleum ether separately. Antimicrobial assay was performed against nine bacterial pathogens (isolated from infected ornamental fish) and two fungal strains. The extracts were further tested for anti-hemolytic activity following standard protocol. The methanol extracts were also tested for its anti-cancer property against human AGS cell line. Results: High antibacterial activity was displayed by the petroleum ether extract of G. verrucosa against Micrococcus sp. ((31.5 ± 6.5) mm)Maximum [(21.5 ± 1.0) mm] anti-fungal activity was exerted by the methanol extracts of S. wightii and G. verrucosa against Candida sp. Acetone extract of G. verrucosa produced better anti-hemolytic activity (96.47% ± 0.31%) than other tested extracts. Methanol extract of both seaweeds failed to produce any anti-cancer activity against human AGS cell line. Quantitative phytochemical analysis revealed elevated levels of alkaloids (56 mg/g), saponins (44.5 mg/g), flavonoids (500 mg/g) and phenols (220 mg/g) in G. verrucosa when compared to S. wightii. Conclusions: The tested macro-algae are good source of bioactive compounds, and G. verrucosa displayed better activity than S. wightii and could be explored further.
