The spread of transgenes and exotic germplasm from planted crops into wild or feral species is a difficult problem for public and regulatory acceptance of genetically engineered plants,particularly for wind-pollinated...The spread of transgenes and exotic germplasm from planted crops into wild or feral species is a difficult problem for public and regulatory acceptance of genetically engineered plants,particularly for wind-pollinated trees such as poplar.We report that overexpression of a poplar homolog of the floral repressor SHORT VEGETATIVE PHASE-LIKE(SVL),a homolog of the Arabidopsis MADS-box repressor SHORT VEGETATIVE PHASE(SVP),delayed the onset of flowering several years in three genotypes of field-grown transgenic poplars.Higher expression of SVL correlated with a delay in flowering onset and lower floral abundance,and did not cause morphologically obvious or statistically significant effects on leaf characteristics,tree form,or stem volume.Overexpression effects on reproductive and vegetative phenology in spring was modest and genotype-specific.Our results suggest that use of SVL and related floral repressors can be useful tools to enable a high level of containment for vegetatively propagated short-rotation woody energy or pulp crops.展开更多
基金This project was supported by Biotechnology Risk Assessment Grant Program competitive grant no.2011-68005-30407 and 2010-33522-21736from the USDA National Institute of Food and Agriculture and Agricultural Research Service,National Science Foundation I/UCRC Center for Advanced Forestry(grant 0736283)+1 种基金USDA-IFAS(grant OREZ-FS671-R)the J.Frank Schmidt Charitable Foundation,and the TBGRC/GREAT TREES industrial cooperative at Oregon State University.
文摘The spread of transgenes and exotic germplasm from planted crops into wild or feral species is a difficult problem for public and regulatory acceptance of genetically engineered plants,particularly for wind-pollinated trees such as poplar.We report that overexpression of a poplar homolog of the floral repressor SHORT VEGETATIVE PHASE-LIKE(SVL),a homolog of the Arabidopsis MADS-box repressor SHORT VEGETATIVE PHASE(SVP),delayed the onset of flowering several years in three genotypes of field-grown transgenic poplars.Higher expression of SVL correlated with a delay in flowering onset and lower floral abundance,and did not cause morphologically obvious or statistically significant effects on leaf characteristics,tree form,or stem volume.Overexpression effects on reproductive and vegetative phenology in spring was modest and genotype-specific.Our results suggest that use of SVL and related floral repressors can be useful tools to enable a high level of containment for vegetatively propagated short-rotation woody energy or pulp crops.
