The small bowel represents one of the main sites for cutaneous melanoma metastasis;however, numerous cases of primary intestinal melanoma have recently been described. In view of this, we present the case of a 39-year...The small bowel represents one of the main sites for cutaneous melanoma metastasis;however, numerous cases of primary intestinal melanoma have recently been described. In view of this, we present the case of a 39-year-old woman admitted for nausea, heartburn, abdominal pain, change in bowel habits and weight loss. Contrast-enhanced CT revealed a small bowel mass. Surgical resection of a 6 cm ileal tumour with regional mesenteric lymphadenectomy and end-to-end anastomosis was performed. Histopathological findings indicated the presence of an ileal melanoma metastasis. Subsequent dermatological examination identified a cutaneous lesion on the right forearm, however no malignant cells were found at the histopathological exam. Whole body PET CT with FDG identified multiple frontal and parietal lesions. Genetic testing was positive for BRAF gene V600 E mutation. The patient underwent multiple neurosurgical procedures for the resection of cerebral metastases. Palliative external radiation and chemotherapy was also attempted. After approximately 2 years after the diagnosis, the patient died following multiple episodes of intracranial hypertension.展开更多
A 1.4Kb DNA fragment containing 3’ flanking sequence of fibroin gene of silkworm, Antheraea pernyi, was obtained from the silk gland’s mRNA of 5 th larva. Analysis of this sequence with another A.pernyi fibroin prot...A 1.4Kb DNA fragment containing 3’ flanking sequence of fibroin gene of silkworm, Antheraea pernyi, was obtained from the silk gland’s mRNA of 5 th larva. Analysis of this sequence with another A.pernyi fibroin protein (accession No. D83241) revealed that it consists of a completely open reading frame (ORF), which includes 14 polyalanine-containing units (motifs) and 100bp 3’-UTR. The sequence of the predicted amino acid reveals the highest level of overall identity (90%) with D83241. It was found that it loses a repeat region at the upstream of TAA codon and some mutations. A putative polyadenylation signal AATAAA tail was found in position 1300, which follows the termination codon.展开更多
A novel stochastic resonance algorithm was employed to enhance the signal-to-noise ratio (SNR) of signals of analytical chemistry. By using a gas chromatographic data set, it was proven that the SNR was greatly impro...A novel stochastic resonance algorithm was employed to enhance the signal-to-noise ratio (SNR) of signals of analytical chemistry. By using a gas chromatographic data set, it was proven that the SNR was greatly improved and the quantitative relationship between concentrations and chromatographic responses remained simultaneously. The linear range was extended beyond the instrumental detection limit.展开更多
The reverse atom transfer radical polymerization(RATRP) of (-)-menthyl methacrylate ((-)-MnMA) with AIBN(AIBN/CuCl2/bipyridine(bipy) or (-)sparteine((-)Sp) =1/2/4) initiating system in THF has been studied. The depen...The reverse atom transfer radical polymerization(RATRP) of (-)-menthyl methacrylate ((-)-MnMA) with AIBN(AIBN/CuCl2/bipyridine(bipy) or (-)sparteine((-)Sp) =1/2/4) initiating system in THF has been studied. The dependence of the specific rotation on molecular weight was investigated.展开更多
The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24E that is N-acetylserine were replace...The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24E that is N-acetylserine were replaced by hT(1 for the constitution of hT(1-RP fusion gene in order to express acetyllated thymosin α 1. And also,the Asn-Gly bond was designed to faciliate isolation of the target protein.The fusion gene was cloned into the expression vector, pPIC/9K. The constructs were transformed into HIS4 mutant strain GS115 by electroporation. Both SDS-PAGE analysis and Western blot analysis indicated that the fusion protein was expressed successfully.展开更多
A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors,...A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.展开更多
The geography information system of the 1303 Hongtong M=8 earthquake has been established. Using the spatial analysis function of GIS, the spatial distribution characteristics of damage and isoseismal of the earthquak...The geography information system of the 1303 Hongtong M=8 earthquake has been established. Using the spatial analysis function of GIS, the spatial distribution characteristics of damage and isoseismal of the earthquake are studied. By comparing with the standard earthquake intensity attenuation relationship, the abnormal damage dis-tribution of the earthquake is found, so the relationship of the abnormal distribution with tectonics, site condition and basin are analyzed. In this paper, the influence on the ground motion generated by earthquake source and the underground structures near source also are studied. The influence on seismic zonation, anti-earthquake design, earthquake prediction and earthquake emergency responding produced by the abnormal density distribution are discussed.展开更多
The atom transfer radical polymerization(ATRP) of (-)-menthyl methacrylate((-)-MnMA) mediated by CuCl/bipyridine and ethyl 2-bromopropionate or 1-phenylethyl bromide in THF system has been studied. The dependence of ...The atom transfer radical polymerization(ATRP) of (-)-menthyl methacrylate((-)-MnMA) mediated by CuCl/bipyridine and ethyl 2-bromopropionate or 1-phenylethyl bromide in THF system has been studied. The dependence of the specific rotation on molecular weight and the CD of Poly((-)-MnMA) thus obtained was investigated.展开更多
文摘The small bowel represents one of the main sites for cutaneous melanoma metastasis;however, numerous cases of primary intestinal melanoma have recently been described. In view of this, we present the case of a 39-year-old woman admitted for nausea, heartburn, abdominal pain, change in bowel habits and weight loss. Contrast-enhanced CT revealed a small bowel mass. Surgical resection of a 6 cm ileal tumour with regional mesenteric lymphadenectomy and end-to-end anastomosis was performed. Histopathological findings indicated the presence of an ileal melanoma metastasis. Subsequent dermatological examination identified a cutaneous lesion on the right forearm, however no malignant cells were found at the histopathological exam. Whole body PET CT with FDG identified multiple frontal and parietal lesions. Genetic testing was positive for BRAF gene V600 E mutation. The patient underwent multiple neurosurgical procedures for the resection of cerebral metastases. Palliative external radiation and chemotherapy was also attempted. After approximately 2 years after the diagnosis, the patient died following multiple episodes of intracranial hypertension.
文摘A 1.4Kb DNA fragment containing 3’ flanking sequence of fibroin gene of silkworm, Antheraea pernyi, was obtained from the silk gland’s mRNA of 5 th larva. Analysis of this sequence with another A.pernyi fibroin protein (accession No. D83241) revealed that it consists of a completely open reading frame (ORF), which includes 14 polyalanine-containing units (motifs) and 100bp 3’-UTR. The sequence of the predicted amino acid reveals the highest level of overall identity (90%) with D83241. It was found that it loses a repeat region at the upstream of TAA codon and some mutations. A putative polyadenylation signal AATAAA tail was found in position 1300, which follows the termination codon.
基金supported by the National Natural Science Foundation of China (No.20075024).
文摘A novel stochastic resonance algorithm was employed to enhance the signal-to-noise ratio (SNR) of signals of analytical chemistry. By using a gas chromatographic data set, it was proven that the SNR was greatly improved and the quantitative relationship between concentrations and chromatographic responses remained simultaneously. The linear range was extended beyond the instrumental detection limit.
文摘The reverse atom transfer radical polymerization(RATRP) of (-)-menthyl methacrylate ((-)-MnMA) with AIBN(AIBN/CuCl2/bipyridine(bipy) or (-)sparteine((-)Sp) =1/2/4) initiating system in THF has been studied. The dependence of the specific rotation on molecular weight was investigated.
文摘The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24E that is N-acetylserine were replaced by hT(1 for the constitution of hT(1-RP fusion gene in order to express acetyllated thymosin α 1. And also,the Asn-Gly bond was designed to faciliate isolation of the target protein.The fusion gene was cloned into the expression vector, pPIC/9K. The constructs were transformed into HIS4 mutant strain GS115 by electroporation. Both SDS-PAGE analysis and Western blot analysis indicated that the fusion protein was expressed successfully.
文摘A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.
文摘The geography information system of the 1303 Hongtong M=8 earthquake has been established. Using the spatial analysis function of GIS, the spatial distribution characteristics of damage and isoseismal of the earthquake are studied. By comparing with the standard earthquake intensity attenuation relationship, the abnormal damage dis-tribution of the earthquake is found, so the relationship of the abnormal distribution with tectonics, site condition and basin are analyzed. In this paper, the influence on the ground motion generated by earthquake source and the underground structures near source also are studied. The influence on seismic zonation, anti-earthquake design, earthquake prediction and earthquake emergency responding produced by the abnormal density distribution are discussed.
文摘The atom transfer radical polymerization(ATRP) of (-)-menthyl methacrylate((-)-MnMA) mediated by CuCl/bipyridine and ethyl 2-bromopropionate or 1-phenylethyl bromide in THF system has been studied. The dependence of the specific rotation on molecular weight and the CD of Poly((-)-MnMA) thus obtained was investigated.
