Offshore oil pollution has caused serious impacts on plants and aquatic organisms in the marine ecosystem.Bioremediation of oil pollution by immobilized bacteria has aroused wide attention due to the high degradation ...Offshore oil pollution has caused serious impacts on plants and aquatic organisms in the marine ecosystem.Bioremediation of oil pollution by immobilized bacteria has aroused wide attention due to the high degradation rate compared with free bacteria.The properties of the carrier for immobilization play an important role in the oil degradation efficiency.In our study,a marine oil degrading bacteria Sp8(Shewanella algae)was selected from sea water,and enteromorpha was used as carrier material for the immobilization of Sp8.In order to increase the hydrophobicity,sodium dodecylbenzene sulfonate(SDBS)was used as modifier to modify the surface of enteromorpha by the dipping method.Sodium alginate was used as the embedding carrier,and anhydrous calcium chloride was used as the cross-linking agent to prepare the SDBS-E immobilized microbial agent by the embedding method.Compared with the degradation rate achieved by free bacteria(78.87±8.29%),the diesel removal rate accomplished by SDBS-E immobilized microbial agent increased to 90.39±1.24%.The analysis of diesel removal mechanism showed that the diesel removal pathway mainly included surface adsorption,internal uptake,and biodegradation.The diesel removal efficiency relied on surface adsorption in the early stage,and then depended on biodegradation in the later stage.The removal of diesel by SDBS-E immobilized microbial agent conformed to the quasi-first-order degradation kinetic model.The results of software-MOE suggested that enteromorpha-immobilized microbial agent adsorbed diesel mainly through hydrogen bonds formed with diesel components.This study can provide a research basis and idea for the practical application of immobilization technology to remove petroleum from seawater in the future.展开更多
基金This study was funded by the scientific research fund project of National Natural Science Foundation of China(Grant No.52070123)the Natural Science Foundation of Shandong Province(grant numbers ZR2020ME224,ZR2019PB031)the Project of Shandong Province Higher Educational Young Innovative Talent Introduction and Cultivation Team[Wastewater treatment and resource innovation team].
文摘Offshore oil pollution has caused serious impacts on plants and aquatic organisms in the marine ecosystem.Bioremediation of oil pollution by immobilized bacteria has aroused wide attention due to the high degradation rate compared with free bacteria.The properties of the carrier for immobilization play an important role in the oil degradation efficiency.In our study,a marine oil degrading bacteria Sp8(Shewanella algae)was selected from sea water,and enteromorpha was used as carrier material for the immobilization of Sp8.In order to increase the hydrophobicity,sodium dodecylbenzene sulfonate(SDBS)was used as modifier to modify the surface of enteromorpha by the dipping method.Sodium alginate was used as the embedding carrier,and anhydrous calcium chloride was used as the cross-linking agent to prepare the SDBS-E immobilized microbial agent by the embedding method.Compared with the degradation rate achieved by free bacteria(78.87±8.29%),the diesel removal rate accomplished by SDBS-E immobilized microbial agent increased to 90.39±1.24%.The analysis of diesel removal mechanism showed that the diesel removal pathway mainly included surface adsorption,internal uptake,and biodegradation.The diesel removal efficiency relied on surface adsorption in the early stage,and then depended on biodegradation in the later stage.The removal of diesel by SDBS-E immobilized microbial agent conformed to the quasi-first-order degradation kinetic model.The results of software-MOE suggested that enteromorpha-immobilized microbial agent adsorbed diesel mainly through hydrogen bonds formed with diesel components.This study can provide a research basis and idea for the practical application of immobilization technology to remove petroleum from seawater in the future.
