In Vitro Antibacterial Effect of Traditional Chinese Medicine on Main Pathogenic Bacteria of Recessive Mastitis in Dairy Cows

The inhibition zone diameter, minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of 20 kinds of traditional Chi- nese medicine on Staphylococcus aureus and Streptococcus agalactiae isolated from recessive mastitis were studied using plate test and tube test. The results showed that S. aureus and S. agalactiae were sensitive to all these 20 kinds of traditional Chinese medicine, and were more sensitive to seven kinds of traditional Chinese medicine, including Taraxtwum mongolicum, Coptis chinensis, Forsythia suspense, Andrographis paniculata, Carthamus tinctorius, Scutellaria baicalensis and Den- dranthema indicum.

Isolation and Identification of Pathogenic Bacteria Causing Bovine Subclinical Mastitis in Eastern Hebei Province

In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclinical mastitis positive milk samples were isolated and identified.The results showed that 60.63%（619/1 021） of the sampled cows were diagnosed with subclinical mastitis, and mixed infections accounted for 88.21%（546/619） of the cases. In addition, 82 strains of 14 species were isolated from the subclinical mastitis positive milk samples, including 36 strains of Staphylococcus（43.90%）, 33 strains of Streptococcus（40.24%）, 8 strains of Enterobacteriaceae（9.76%） and 5 strains of Corynebacterium（6.10%）, respectively. The results proved that Staphylococcus aureus and Streptococcus agalactiae are the main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province.

心肌桥所致冠状动脉迂曲与冠状动脉粥样硬化的相关性分析

目的分析心肌桥(MB)所致冠状动脉迂曲与冠状动脉粥样硬化的相关性。方法回顾性分析华北理工大学附属医院2015年3月至2016年6月疑似冠心病经CT冠状动脉成像检查发现MB所致冠状动脉迂曲的88例患者资料,另选取同时期经CT冠状动脉成像检查发现冠状动脉迂曲而无MB的88例患者资料作为对照组。观察冠状动脉粥样硬化及狭窄情况,收集患者的年龄、性别、高血压、高血脂、糖尿病、吸烟史、家族史及体质量指数。比较MB组与非MB组整支冠状动脉、迂曲前段、迂曲段、迂曲后段冠状动脉粥样硬化发生率;比较MB组与非MB组冠状动脉迂曲前段、迂曲段、迂曲后段冠状动脉狭窄程度;比较两组年龄、性别、血压、血脂、血糖、吸烟史、家族史及体质量指数等方面的差异。对不同迂曲程度分级中完全MB组、不完全MB组及非MB组迂曲前段冠脉粥样硬化发生率的差异进行两两比较。结果 MB组整支(χ~2=15.565,P=0.000)、迂曲前段(χ~2=8.606,P=0.003)、迂曲段(χ~2=13.475,P=0.000)及迂曲后段(χ~2=4.314,P=0.038)冠脉粥样硬化发生率均低于非MB组,差异有统计学意义。MB组迂曲前段(χ~2=6.988,P=0.008)、迂曲段(χ~2=10.050,P=0.002)及迂曲后段(χ~2=4.314,P=0.038)轻度狭窄发生率均低于非MB组,差异有统计学意义。两组迂曲前段(χ~2=0.586,P=0.444)、迂曲段(χ~2=1.356,P=0.244)及迂曲后段中重度狭窄发生率差异无统计学意义(P>0.05)。完全MB组在轻度(χ~2=13.526,P=0.000)及中度迂曲(Fisher确切概率P=0.015)中迂曲前段冠脉粥样硬化发生率低于非MB组,差异均具有统计学意义;而在重度迂曲(Fisher确切概率P=0.155)中两组差异无统计学意义(P>0.0167)。不完全MB组在轻度迂曲中迂曲前段冠脉粥样硬化发生率低于非MB组,差异均具有统计学意义(χ~2=9.167,P=0.002);而在中度(Fisher确切概率P=0.047)及重度迂曲(Fisher确切概率P=0.031)中两组差异均无统计学意义(P>0.0167)。完全MB组与不完全MB组在轻度(χ~2=1.239,P=0.266)、中度(Fisher确切概率P=1.000)及重度迂曲(Fisher确切概率P=1.000)中迂曲前段冠脉粥样硬化发生率差异均无统计学意义。结论与非MB者相比,MB尤其是完全MB所致冠状动脉迂曲者冠状动脉粥样硬化发生率较低,冠脉轻度狭窄发生率也较低,但其与冠脉中重度狭窄发生率无相关性。

Influences of Echinacea Polysaccharide on Secretion of IL-8mRNA by LPS-injured IEC-6 Cells

[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was extracted with TRlzon reagent. IL-8 mRNA was amplified by RT-PCR and detected by agar- ose gel electrophoresis. Furthermore, electrophoresis and image was analyzed. [ Result] The 50 μg/mL EPS could partially inhibit IL-8 mRNA level produced by -stimulated IEC-6; with the increasing concentration of EPS （200 and 500 μg/mL） , the inhibitory effect against IL-8 mRNA gradually enhanced. IEC-6 was pretreated by 50, 100,200 and 500 μg/mL EPS for ：24 h, then stimulated by 10 μg/ml 1,PS for 1 and 4 h, respectively. RT-PCR analysis showed that the expres- sion of 1L-8 mRNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8mRNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at I h. [ Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 mRNA by LPS-stimulated cells, and the inhibition of EPS was dependent to concentration and time.