目的观察超像素引导含双流基于局部图像判别器生成对抗网络(SPDD-PatchGAN)用于分割皮肤镜图像中皮肤病变的价值。方法分别于国际皮肤成像合作组织(ISIC)2016数据集及Human Against Machine with 10000 training images(HAM10000)数据...目的观察超像素引导含双流基于局部图像判别器生成对抗网络(SPDD-PatchGAN)用于分割皮肤镜图像中皮肤病变的价值。方法分别于国际皮肤成像合作组织(ISIC)2016数据集及Human Against Machine with 10000 training images(HAM10000)数据集中收集1279及10015幅皮肤病变患者的皮肤镜图像,以数据集中的手动分割结果为参考标准,利用包含多尺度上下文提取模块(MCEM)的残差注意力UNet(RA-UNet)为生成器,采用基于局部图像的超像素引导的双流判别策略为判别器构建SPDD-PatchGAN并以之分割图像中的皮肤病变;与深度卷积生成对抗网络(DCGAN)、UNet、Attention-UNet、上下文编码器网络(CENet)、上下文金字塔融合网络(CPFNet)和生成对抗模型双流生成对抗网络(DAGAN)对比,采用平均交并比(mIoU)、准确率(Accuracy)、召回率(Recall)评价SPDD-PatchGAN的分割效能。结果SPDD-PatchGAN分割皮肤病变的整体效果较佳,且其mIoU、Accuracy及Recall均优于DCGAN、UNet、Attention-UNet、CENet、CPFNet及DAGAN。结论SPDD-PatchGAN可有效分割皮肤镜图像中的皮肤病变。展开更多
BACKGROUND:Plenty of studies have demonstrated that inflammatory reaction is involved in ischemic cerebral damage,and the expression of inflammatory cytokines can be observed at the initial sites of cerebral damage at...BACKGROUND:Plenty of studies have demonstrated that inflammatory reaction is involved in ischemic cerebral damage,and the expression of inflammatory cytokines can be observed at the initial sites of cerebral damage at early period,including interleukin-6,interleukin-8,etc.,which are all the target gene products of nuclear factor kappa B(NF-κB).The process of ischemic damage can be affected by adjusting and controlling NF-κB activity via multi-links.OBJECTIVE:To investigate the inhibitory effect of interleukin-10 on the expression of NF-κB in the ischemic sites of rats with focal cerebral ischemia in rats and its molecular mechanisms.DESIGN:A randomized and controlled animal trial.SETTING:Department of Neurology,the Affiliated Union Hospital of Fujian Medical University.MATERIALS:Thirty-two adult male Sprague-Dawley rats weighing(250±30)g were used.NF-κB p65(RelA)rabbit anti-rat monoclonal primary antibody was the product of Neomarkers Company;Immunohistochemical kit of the SP two-step method was purchased from Beijing Zhongshan Biotechnology Co.,Ltd.METHODS:The experiment was carried out in the Affiliated Union Hospital of Fujian Medical University from August 2005 to April 2006.The rats were randomly assigned into sham-operated group,middle cerebral artery occlusion(MCAO)group,vehicle-treated group and interleukin-10 treated group,8 rats in each group.Focal cerebral ischemia was induced by occlusion of the middle cerebral artery as previously described.Rats in the MCAO group were anesthetized intraperitoneally,thyroid was bluntly dissected.Right common,external and internal carotid arteries were isolated,the trunk of external carotid artery was ligated and freed,an artery clamp was placed at the internal carotid artery,then a'V'shape incision was made at the free section of external carotid artery,filament was inserted for a depth of(18.5±0.5)mm.The rats in the sham-operated group were given the same treatments with the exception of filament insertion.After the successful model establishment for 1 hour,the rats in the interleukin-10 treated group were injected with human recombinant interleukin-10(1μg)via lateral ventricle,whereas those in the vehicle-treated group were injected with 5 mol/L NaP(5μL).The rectal temperature of rats were kept at about 37℃with heating lamps throughout the operation.Twenty-four hours after MCAO,the rats were examined for neurological deficits.Only those animals that scored at 1-3 points were utilized.The rats were decapitated at 24 hours postoperatively.The expression of NF-κB p65 in peri-infarct core was detected immunohistochemically.The percentage of NF-κB p65 subunit positive cells in 1000 cells was calculated.MAIN OUTCOME MEASURES:Expression of NF-κB p65 in peri-infarct core;Percentage of NF-κB p65 subunit positive cells.RESULTS:All the 32 rats were involved in the analysis of results.NF-κB p65 expressed in cytoplasm and some nuclei.It was expressed all in cytoplasm in the sham-operated group,and partly expressed in the nucleus after cerebral ischemia.Small amounts of NF-κB p65 positive neurocytes were observed in the sham-operated group[(3.7±0.6)%],those were obviously increased in the MCAO group[(15.4±3.7)%,P<0.01].NF-κB p65 positive neurocytes were significantly reduced in the interleukin-10 treated group as compared with those in the vehicle-treated groups[(12.1±2.2)%,(15.5±3.6)%,P<0.05].CONCLUSION:Interleukin-10 injected via lateral ventricle can effectively inhibit the expression of NF-κB p65 in the peri-ischemic core in rats,and block the gene transcription involved in the inflammatory cascade reaction.展开更多
At the invitation of the Hameenlinna Cultural Centre of Finland, the Folklore Association of the Czech Republic, the Belarus Society for Friendship and Cultural Relations with Foreign Countries, Saku Music School of E...At the invitation of the Hameenlinna Cultural Centre of Finland, the Folklore Association of the Czech Republic, the Belarus Society for Friendship and Cultural Relations with Foreign Countries, Saku Music School of Estonia and the Chil-展开更多
文摘目的观察超像素引导含双流基于局部图像判别器生成对抗网络(SPDD-PatchGAN)用于分割皮肤镜图像中皮肤病变的价值。方法分别于国际皮肤成像合作组织(ISIC)2016数据集及Human Against Machine with 10000 training images(HAM10000)数据集中收集1279及10015幅皮肤病变患者的皮肤镜图像,以数据集中的手动分割结果为参考标准,利用包含多尺度上下文提取模块(MCEM)的残差注意力UNet(RA-UNet)为生成器,采用基于局部图像的超像素引导的双流判别策略为判别器构建SPDD-PatchGAN并以之分割图像中的皮肤病变;与深度卷积生成对抗网络(DCGAN)、UNet、Attention-UNet、上下文编码器网络(CENet)、上下文金字塔融合网络(CPFNet)和生成对抗模型双流生成对抗网络(DAGAN)对比,采用平均交并比(mIoU)、准确率(Accuracy)、召回率(Recall)评价SPDD-PatchGAN的分割效能。结果SPDD-PatchGAN分割皮肤病变的整体效果较佳,且其mIoU、Accuracy及Recall均优于DCGAN、UNet、Attention-UNet、CENet、CPFNet及DAGAN。结论SPDD-PatchGAN可有效分割皮肤镜图像中的皮肤病变。
基金grants from Fujian Bureau of Education,No.C0440009Fujian Bureau of Science and Technology,No.2005K046
文摘BACKGROUND:Plenty of studies have demonstrated that inflammatory reaction is involved in ischemic cerebral damage,and the expression of inflammatory cytokines can be observed at the initial sites of cerebral damage at early period,including interleukin-6,interleukin-8,etc.,which are all the target gene products of nuclear factor kappa B(NF-κB).The process of ischemic damage can be affected by adjusting and controlling NF-κB activity via multi-links.OBJECTIVE:To investigate the inhibitory effect of interleukin-10 on the expression of NF-κB in the ischemic sites of rats with focal cerebral ischemia in rats and its molecular mechanisms.DESIGN:A randomized and controlled animal trial.SETTING:Department of Neurology,the Affiliated Union Hospital of Fujian Medical University.MATERIALS:Thirty-two adult male Sprague-Dawley rats weighing(250±30)g were used.NF-κB p65(RelA)rabbit anti-rat monoclonal primary antibody was the product of Neomarkers Company;Immunohistochemical kit of the SP two-step method was purchased from Beijing Zhongshan Biotechnology Co.,Ltd.METHODS:The experiment was carried out in the Affiliated Union Hospital of Fujian Medical University from August 2005 to April 2006.The rats were randomly assigned into sham-operated group,middle cerebral artery occlusion(MCAO)group,vehicle-treated group and interleukin-10 treated group,8 rats in each group.Focal cerebral ischemia was induced by occlusion of the middle cerebral artery as previously described.Rats in the MCAO group were anesthetized intraperitoneally,thyroid was bluntly dissected.Right common,external and internal carotid arteries were isolated,the trunk of external carotid artery was ligated and freed,an artery clamp was placed at the internal carotid artery,then a'V'shape incision was made at the free section of external carotid artery,filament was inserted for a depth of(18.5±0.5)mm.The rats in the sham-operated group were given the same treatments with the exception of filament insertion.After the successful model establishment for 1 hour,the rats in the interleukin-10 treated group were injected with human recombinant interleukin-10(1μg)via lateral ventricle,whereas those in the vehicle-treated group were injected with 5 mol/L NaP(5μL).The rectal temperature of rats were kept at about 37℃with heating lamps throughout the operation.Twenty-four hours after MCAO,the rats were examined for neurological deficits.Only those animals that scored at 1-3 points were utilized.The rats were decapitated at 24 hours postoperatively.The expression of NF-κB p65 in peri-infarct core was detected immunohistochemically.The percentage of NF-κB p65 subunit positive cells in 1000 cells was calculated.MAIN OUTCOME MEASURES:Expression of NF-κB p65 in peri-infarct core;Percentage of NF-κB p65 subunit positive cells.RESULTS:All the 32 rats were involved in the analysis of results.NF-κB p65 expressed in cytoplasm and some nuclei.It was expressed all in cytoplasm in the sham-operated group,and partly expressed in the nucleus after cerebral ischemia.Small amounts of NF-κB p65 positive neurocytes were observed in the sham-operated group[(3.7±0.6)%],those were obviously increased in the MCAO group[(15.4±3.7)%,P<0.01].NF-κB p65 positive neurocytes were significantly reduced in the interleukin-10 treated group as compared with those in the vehicle-treated groups[(12.1±2.2)%,(15.5±3.6)%,P<0.05].CONCLUSION:Interleukin-10 injected via lateral ventricle can effectively inhibit the expression of NF-κB p65 in the peri-ischemic core in rats,and block the gene transcription involved in the inflammatory cascade reaction.
文摘At the invitation of the Hameenlinna Cultural Centre of Finland, the Folklore Association of the Czech Republic, the Belarus Society for Friendship and Cultural Relations with Foreign Countries, Saku Music School of Estonia and the Chil-
