The purpose of this study was to evaluate the relationship of tetrazolium salt testing to common indicators(milk line,black layer,dry matter)used to determine corn seed physiological maturity.The seeds used were the h...The purpose of this study was to evaluate the relationship of tetrazolium salt testing to common indicators(milk line,black layer,dry matter)used to determine corn seed physiological maturity.The seeds used were the hybrids Pioneer 4285 and Dow 2B587.These hybrids were harvested 40 days after polinization(DAP),with 4-day intervals until 68 DAP.The harvested seeds were evaluated for viability and vigor(germination test,accelerated aging test and cold test).The parameters used to determine the physiological seed’s maturity were the black layer,the milk line,the seed dry matter,seed water content and the evaluation of seed tissues using the tetrazolium salt to evaluate the endosperm cells.The germination of both materials was greater than 95%and there was no difference between harvest times.Corn seed physiological maturity(PM)was identified at 56 DAP for P4285(F2)and between 48 and 56 DAP for 2B587(F2).The maximum vigor was detected by accelerated aging tests and cold tests before PM for the two genotypes.The activity of endosperm cells is related to the other PM indicators(milk line,black layer,dry matter and water content).The transport of nutrients from plant to the seed ceases at seed physiological maturity.Use of tetrazolium salt allows identifying cell death of the basal region and this parameter corresponds to the others evaluated,thus demonstrating that the tetrazolium salt is effective for corn seed physiological maturity characterization.展开更多
文摘The purpose of this study was to evaluate the relationship of tetrazolium salt testing to common indicators(milk line,black layer,dry matter)used to determine corn seed physiological maturity.The seeds used were the hybrids Pioneer 4285 and Dow 2B587.These hybrids were harvested 40 days after polinization(DAP),with 4-day intervals until 68 DAP.The harvested seeds were evaluated for viability and vigor(germination test,accelerated aging test and cold test).The parameters used to determine the physiological seed’s maturity were the black layer,the milk line,the seed dry matter,seed water content and the evaluation of seed tissues using the tetrazolium salt to evaluate the endosperm cells.The germination of both materials was greater than 95%and there was no difference between harvest times.Corn seed physiological maturity(PM)was identified at 56 DAP for P4285(F2)and between 48 and 56 DAP for 2B587(F2).The maximum vigor was detected by accelerated aging tests and cold tests before PM for the two genotypes.The activity of endosperm cells is related to the other PM indicators(milk line,black layer,dry matter and water content).The transport of nutrients from plant to the seed ceases at seed physiological maturity.Use of tetrazolium salt allows identifying cell death of the basal region and this parameter corresponds to the others evaluated,thus demonstrating that the tetrazolium salt is effective for corn seed physiological maturity characterization.
