AIM: To study the effect of oral steroids upon clinical response and rectal mucosa secretion of eosinophil cationic protein (ECP), myeloperoxidase (MPO), basic fibroblast growth factor (bFGF), vascular endothelial gro...AIM: To study the effect of oral steroids upon clinical response and rectal mucosa secretion of eosinophil cationic protein (ECP), myeloperoxidase (MPO), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and albumin in patients with collagenous colitis (CC). METHODS: A segmental perfusion technique was used to collect perfusates from rectum of CC patients once before and twice (one and four weeks) after the start of steroid treatment. Clinical data was monitored and ECP, MPO, bFGF, VEGF and albumin concentrations were analyzed by immunochemical methods in perfusates and in serum. RESULTS: Steroids reduced the number of bowel movements by more than five times within one week and all patients reported improved subjective well- being at wk 1 and 4. At the same time, the median concentrations of ECP, bFGF, VEGF and albumin in rectal perfusates decreased significantly. MPO values were above the detection limit in only 3 patients before treatment and in none during treatment. VEGF, bFGF, ECP and albumin concentrations correlated with each other with the exception of ECP and albumin. A decrease of serum ECP and VEGF concentrations was also seen even if the overtime reduction was not significant.CONCLUSION: Oral steroid treatment in CC patients induced a simultaneous reduction of bowel movements and rectal release of ECP, bFGF, VEGF and albumin, suggesting that these polypeptides and increased mucosal permeability are important components of the pathophysiology in collagenous colitis.展开更多
BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is a common disorder,with an estimated prevalence ranging from 20%to 35%in the general population.Several scores based on easily measurable biochemical and clinical p...BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is a common disorder,with an estimated prevalence ranging from 20%to 35%in the general population.Several scores based on easily measurable biochemical and clinical parameters,including the fatty liver index(FLI),hepatic steatosis index(HSI),lipid accumulation product(LAP),and NAFLD liver fat score(LFS),have been developed for the detection of NAFLD.However,comparative information regarding the efficacy of these scores for predicting NAFLD in population-based samples comprising normal and high-risk individuals is lacking.AIM To evaluate four NAFLD detection scores in two samples with different NAFLD risks.METHODS NAFLD screening was performed in a population-based sample of 50-year-old individuals in Uppsala,Sweden[n=310;Prospective investigation of obesity,energy and metabolism(POEM)study]and a high-risk population comprising patients with a body mass index>25 kg/m2 and either high plasma triglycerides(≥1.7 mmol/L)or type 2 diabetes(n=310;EFFECT studies).NAFLD was defined as liver fat>5.5%using magnetic resonance imaging-proton density fat fraction.FLI,HSI,LAP,and NAFLD LFS were assessed.A logistic regression model was used to evaluate the effectiveness of the different scores.RESULTS The prevalence of NAFLD was 23%in POEM.FLI showed the highest receiver operating characteristic area under the curve(ROC AUC;0.82)and was significantly better than the LAP score(P=0.005 vs LAP,P=0.08 vs LFS,P=0.12 vs HSI)for detection of NAFLD.The other three indices performed equally in POEM(0.77-0.78).The prevalence of NAFLD was 74%in EFFECT;LFS performed best(ROC AUC 0.80)in this sample.The ROC AUC for LFS(0.80)was significantly higher than that for FLI(P=0.0019)and LAP(P=0.0022),but not HSI(P=0.11).We performed a sensitivity analysis with stratification for the two high-risk subgroups(patients with diabetes or hypertriglyceridemia)from the EFFECT studies.LAP performed best in patients with hypertriglyceridemia.No major differences were observed between the other scores.CONCLUSION The four investigated NAFLD scores performed differently in the populationbased vs high-risk setting.FLI was preferable in the population-based setting,while LFS performed best in the high-risk setting.展开更多
Objective: To examine the correlation between plasma hCG results obtained with the new i-STAT® hCG point of care test with those concomitantly obtained from the central hospital laboratory utilizing the same pa...Objective: To examine the correlation between plasma hCG results obtained with the new i-STAT® hCG point of care test with those concomitantly obtained from the central hospital laboratory utilizing the same patient samples. Methods: Prospective cross-sectional laboratory test evaluation. We compared plasma hCG results obtained with the i-STAT® hCG test (Abbott Point of Care, Princeton, NJ, USA) with Architect Ci8200 (Abbott Laboratories, Abbott Park, IL, USA). We also calculated the total coefficient of variation (CV) for the i-STAT® method. Results: The two methods showed a good linear correlation (R2 = 0.994;slope 1.03) and CV for the i-STAT® method was 2.1% - 5.2%. Conclusion: We suggest that the i-STAT® hCG blood assay could be used as a complement to urine hCG assays in clinical situations when rapid test results are needed and urine is not available.展开更多
Flat metaoptics components are looking to replace classical optics elements and could lead to extremely compact biophotonics devices if integrated with on-chip light sources and detectors.However,using metasurfaces to...Flat metaoptics components are looking to replace classical optics elements and could lead to extremely compact biophotonics devices if integrated with on-chip light sources and detectors.However,using metasurfaces to shape light into wide angular range wavefronts with high efficiency,as is typically required in high-contrast microscopy applications,remains a challenge.Here we demonstrate curved GaAs metagratings integrated on vertical-cavity surface-emitting lasers(VCSELs)that enable on-chip illumination in total internal reflection and dark field microscopy.Based on an unconventional design that circumvents the aspect ratio dependent etching problems in monolithic integration,we demonstrate off-axis emission centred at 60°in air and 63°in glass with>90%and>70%relative deflection efficiency,respectively.The resulting laser beam is collimated out-of-plane but maintains Gaussian divergence in-plane,resulting in a long and narrow illumination area.We show that metagrating-integrated VCSELs of different kinds can be combined to enable rapid switching between dark-field and total internal reflection illumination.Our approach provides a versatile illumination solution for high-contrast imaging that is compatible with conventional microscopy setups and can be integrated with biophotonics devices,such as portable microscopy,NIR-II range bioimaging,and lab-on-a-chip devices.展开更多
基金Supported by grants from the Swedish Medical Research Council, the Swedish Society of Medicine, the Medical Faculty of Uppsala University and Department of Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge
文摘AIM: To study the effect of oral steroids upon clinical response and rectal mucosa secretion of eosinophil cationic protein (ECP), myeloperoxidase (MPO), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and albumin in patients with collagenous colitis (CC). METHODS: A segmental perfusion technique was used to collect perfusates from rectum of CC patients once before and twice (one and four weeks) after the start of steroid treatment. Clinical data was monitored and ECP, MPO, bFGF, VEGF and albumin concentrations were analyzed by immunochemical methods in perfusates and in serum. RESULTS: Steroids reduced the number of bowel movements by more than five times within one week and all patients reported improved subjective well- being at wk 1 and 4. At the same time, the median concentrations of ECP, bFGF, VEGF and albumin in rectal perfusates decreased significantly. MPO values were above the detection limit in only 3 patients before treatment and in none during treatment. VEGF, bFGF, ECP and albumin concentrations correlated with each other with the exception of ECP and albumin. A decrease of serum ECP and VEGF concentrations was also seen even if the overtime reduction was not significant.CONCLUSION: Oral steroid treatment in CC patients induced a simultaneous reduction of bowel movements and rectal release of ECP, bFGF, VEGF and albumin, suggesting that these polypeptides and increased mucosal permeability are important components of the pathophysiology in collagenous colitis.
文摘BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is a common disorder,with an estimated prevalence ranging from 20%to 35%in the general population.Several scores based on easily measurable biochemical and clinical parameters,including the fatty liver index(FLI),hepatic steatosis index(HSI),lipid accumulation product(LAP),and NAFLD liver fat score(LFS),have been developed for the detection of NAFLD.However,comparative information regarding the efficacy of these scores for predicting NAFLD in population-based samples comprising normal and high-risk individuals is lacking.AIM To evaluate four NAFLD detection scores in two samples with different NAFLD risks.METHODS NAFLD screening was performed in a population-based sample of 50-year-old individuals in Uppsala,Sweden[n=310;Prospective investigation of obesity,energy and metabolism(POEM)study]and a high-risk population comprising patients with a body mass index>25 kg/m2 and either high plasma triglycerides(≥1.7 mmol/L)or type 2 diabetes(n=310;EFFECT studies).NAFLD was defined as liver fat>5.5%using magnetic resonance imaging-proton density fat fraction.FLI,HSI,LAP,and NAFLD LFS were assessed.A logistic regression model was used to evaluate the effectiveness of the different scores.RESULTS The prevalence of NAFLD was 23%in POEM.FLI showed the highest receiver operating characteristic area under the curve(ROC AUC;0.82)and was significantly better than the LAP score(P=0.005 vs LAP,P=0.08 vs LFS,P=0.12 vs HSI)for detection of NAFLD.The other three indices performed equally in POEM(0.77-0.78).The prevalence of NAFLD was 74%in EFFECT;LFS performed best(ROC AUC 0.80)in this sample.The ROC AUC for LFS(0.80)was significantly higher than that for FLI(P=0.0019)and LAP(P=0.0022),but not HSI(P=0.11).We performed a sensitivity analysis with stratification for the two high-risk subgroups(patients with diabetes or hypertriglyceridemia)from the EFFECT studies.LAP performed best in patients with hypertriglyceridemia.No major differences were observed between the other scores.CONCLUSION The four investigated NAFLD scores performed differently in the populationbased vs high-risk setting.FLI was preferable in the population-based setting,while LFS performed best in the high-risk setting.
文摘Objective: To examine the correlation between plasma hCG results obtained with the new i-STAT® hCG point of care test with those concomitantly obtained from the central hospital laboratory utilizing the same patient samples. Methods: Prospective cross-sectional laboratory test evaluation. We compared plasma hCG results obtained with the i-STAT® hCG test (Abbott Point of Care, Princeton, NJ, USA) with Architect Ci8200 (Abbott Laboratories, Abbott Park, IL, USA). We also calculated the total coefficient of variation (CV) for the i-STAT® method. Results: The two methods showed a good linear correlation (R2 = 0.994;slope 1.03) and CV for the i-STAT® method was 2.1% - 5.2%. Conclusion: We suggest that the i-STAT® hCG blood assay could be used as a complement to urine hCG assays in clinical situations when rapid test results are needed and urine is not available.
基金This work was funded by the Knut and Alice Wallenberg Foundation and performed in part at Myfab Chalmers.The authors acknowledge Emelie Tornéus for assistance preparing the cell samples and Ruggero Verre for assistance with the nanofabrication.Cell samples were a kind gift from Caroline Adiels.Nanorods were synthesized by Lei Shao.
文摘Flat metaoptics components are looking to replace classical optics elements and could lead to extremely compact biophotonics devices if integrated with on-chip light sources and detectors.However,using metasurfaces to shape light into wide angular range wavefronts with high efficiency,as is typically required in high-contrast microscopy applications,remains a challenge.Here we demonstrate curved GaAs metagratings integrated on vertical-cavity surface-emitting lasers(VCSELs)that enable on-chip illumination in total internal reflection and dark field microscopy.Based on an unconventional design that circumvents the aspect ratio dependent etching problems in monolithic integration,we demonstrate off-axis emission centred at 60°in air and 63°in glass with>90%and>70%relative deflection efficiency,respectively.The resulting laser beam is collimated out-of-plane but maintains Gaussian divergence in-plane,resulting in a long and narrow illumination area.We show that metagrating-integrated VCSELs of different kinds can be combined to enable rapid switching between dark-field and total internal reflection illumination.Our approach provides a versatile illumination solution for high-contrast imaging that is compatible with conventional microscopy setups and can be integrated with biophotonics devices,such as portable microscopy,NIR-II range bioimaging,and lab-on-a-chip devices.
