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Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background 被引量:7
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作者 柴建芳 吴志明 +2 位作者 赵和 andre laroche 王海波 《Acta Botanica Sinica》 CSCD 2003年第4期379-383,共5页
为解决AFLP中样品间共同片段对多态性片段识别的干扰 ,作者建立了一种“减法AFLP”标记技术 ,并运用这种技术成功对小麦中的外源染色体片段进行了标记。运用该技术 ,样品间的共同扩增片段显著减少 ,在非变性聚丙稀酰胺凝胶上带型差异十... 为解决AFLP中样品间共同片段对多态性片段识别的干扰 ,作者建立了一种“减法AFLP”标记技术 ,并运用这种技术成功对小麦中的外源染色体片段进行了标记。运用该技术 ,样品间的共同扩增片段显著减少 ,在非变性聚丙稀酰胺凝胶上带型差异十分明显 ,多态性片段很容易分辨。其真实性通过将其中两条成功转换成外源染色体片段特异的SCAR标记得到了验证。减法AFLP标记技术的建立为标记作物中的外源染色体片段提供了一种有效手段。 展开更多
关键词 subtracted AFLP subtractive hybridization AFLP alien chromosome fragment WHEAT
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The Stripe Rust Resistance Gene Yr10 Encodes an Evolutionary-Conserved and Unique CC-NBS-LRR Sequence in Wheat 被引量:27
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作者 Wei Liu Michele Frick +8 位作者 Rene Huel Cory L. Nykiforuk Xiaomin Wang Denis A. Gaudet Francois Eudes Robert L. Conner Alan Kuzyk Zhensheng Kang andre laroche 《Molecular Plant》 SCIE CAS CSCD 2014年第12期1740-1755,共16页
The first seedling or all-stage resistance (R) R gene against stripe rust isolated from Moro wheat (Triticum aes- tivum L.) using a map-based cloning approach was identified as Yr10. Clone 4B of this gene encodes ... The first seedling or all-stage resistance (R) R gene against stripe rust isolated from Moro wheat (Triticum aes- tivum L.) using a map-based cloning approach was identified as Yr10. Clone 4B of this gene encodes a highly evolutionary- conserved and unique CC-NBS-LRR sequence. Clone 4E, a homolog of Yr10, but lacking transcription start site (TSS) and putative TATA-box and CAAT-box, is likely a non-expressed pseudogene. Clones 4B and 4E are 84% identical and divergent in the intron and the LRR domain. Gene silencing and transgenesis were used in conjunction with inoculation with differen- tially avirulent and virulent stripe rust strains to demonstrate Yr10 functionality. The Yr10 CC-NBS-LRR sequence is unique among known CC-NBS-LRR R genes in wheat but highly conserved homologs (E = 0.0) were identified in Aegilops tauschii and other monocots including Hordeum vulgare and Brachypodium distachyon. Related sequences were also identified in genomic databases of maize, rice, and in sorghum. This is the first report of a CC-NBS-LRR resistance gene in plants with limited homologies in its native host, but with numerous homologous R genes in related monocots that are either host or non-hosts for stripe rust. These results represent a unique example of gene evolution and dispersion across species. 展开更多
关键词 seedling or all-stage stripe rust resistance gene functionality TRANSGENESIS gene silencing homolog sequences coiled-coil region nucleot de-b nding site leucine-rich repeat domain.
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