Tomato(Solanum lycopersicum)fruits are typically red at ripening,with high levels of carotenoids and a low content in flavonoids.Considerable work has been done to enrich the spectrum of their healthbeneficial phytoch...Tomato(Solanum lycopersicum)fruits are typically red at ripening,with high levels of carotenoids and a low content in flavonoids.Considerable work has been done to enrich the spectrum of their healthbeneficial phytochemicals,and interspecific crosses with wild species have successfully led to purple anthocyanin-colored fruits.The Aft(Anthocyanin fruit)tomato accession inherited from Solanum chilense the ability to accumulate anthocyanins in fruit peel through the introgression of loci controlling anthocyanin pigmentation,including four R2R3 MYB transcription factor-encoding genes.Here,we carried out a comparative functional analysis of these transcription factors in wild-type and Aft plants,and tested their ability to take part in the transcriptional complexes that regulate the biosynthetic pathway and their effi-ciency in inducing anthocyanin pigmentation.Significant differences emerged for SlAN2like,both in the expression level and protein functionality,with splicing mutations determining a complete loss of function of the wild-type protein.This transcription factor thus appears to play a key role in the anthocyanin fruit pigmentation.Our data provide new clues to the long-awaited genetic basis of the Aft phenotype and contribute to understand why domesticated tomato fruits display a homogeneous red coloration without the typical purple streaks observed in wild tomato species.展开更多
The effects of the plant hormone auxin have been widely studied over many decades.Auxin regulates diverse processes in plants,and auxin signaling is known to play key roles in both plant development and plant response...The effects of the plant hormone auxin have been widely studied over many decades.Auxin regulates diverse processes in plants,and auxin signaling is known to play key roles in both plant development and plant response to environmental cues(Paque and Weijers,2016).To date,most of the reports dealing with perturbations of auxin signaling relied on auxin sensors based on transcriptional readouts(i.e.,changes in the output level of the reporter due to transcriptional effects of auxins).These sensors,mostly based on the DR5 or the Dll reporter systems,have enabled broad characterization of auxin distributions under various developmental or stress conditions.展开更多
Stomatal closure upon pathogen attack is a process involving Ca2+entry into guard cells after perception of pathogen-associated molecular patterns(PAMPs),small microbial molecules recognized by plant receptors.A centr...Stomatal closure upon pathogen attack is a process involving Ca2+entry into guard cells after perception of pathogen-associated molecular patterns(PAMPs),small microbial molecules recognized by plant receptors.A central role in this process is played by the signal transducing kinase BIK1(Monaghan et al.,2015),which regulates cytosolic Ca2+entry and reactive oxygen species production.However,the Ca2+channel responsible for pathogen-induced cytosolic Ca2+increase in guard cells remains unknown.Recently,in a Nature paper,Thor et al.(2020)reported the characterization of an Arabidopsis Ca2+-permeable channel,OSCA1.3,which is regulated by the BIK1 kinase to participate in this adaptive response(Figure 1).展开更多
文摘Tomato(Solanum lycopersicum)fruits are typically red at ripening,with high levels of carotenoids and a low content in flavonoids.Considerable work has been done to enrich the spectrum of their healthbeneficial phytochemicals,and interspecific crosses with wild species have successfully led to purple anthocyanin-colored fruits.The Aft(Anthocyanin fruit)tomato accession inherited from Solanum chilense the ability to accumulate anthocyanins in fruit peel through the introgression of loci controlling anthocyanin pigmentation,including four R2R3 MYB transcription factor-encoding genes.Here,we carried out a comparative functional analysis of these transcription factors in wild-type and Aft plants,and tested their ability to take part in the transcriptional complexes that regulate the biosynthetic pathway and their effi-ciency in inducing anthocyanin pigmentation.Significant differences emerged for SlAN2like,both in the expression level and protein functionality,with splicing mutations determining a complete loss of function of the wild-type protein.This transcription factor thus appears to play a key role in the anthocyanin fruit pigmentation.Our data provide new clues to the long-awaited genetic basis of the Aft phenotype and contribute to understand why domesticated tomato fruits display a homogeneous red coloration without the typical purple streaks observed in wild tomato species.
文摘The effects of the plant hormone auxin have been widely studied over many decades.Auxin regulates diverse processes in plants,and auxin signaling is known to play key roles in both plant development and plant response to environmental cues(Paque and Weijers,2016).To date,most of the reports dealing with perturbations of auxin signaling relied on auxin sensors based on transcriptional readouts(i.e.,changes in the output level of the reporter due to transcriptional effects of auxins).These sensors,mostly based on the DR5 or the Dll reporter systems,have enabled broad characterization of auxin distributions under various developmental or stress conditions.
文摘Stomatal closure upon pathogen attack is a process involving Ca2+entry into guard cells after perception of pathogen-associated molecular patterns(PAMPs),small microbial molecules recognized by plant receptors.A central role in this process is played by the signal transducing kinase BIK1(Monaghan et al.,2015),which regulates cytosolic Ca2+entry and reactive oxygen species production.However,the Ca2+channel responsible for pathogen-induced cytosolic Ca2+increase in guard cells remains unknown.Recently,in a Nature paper,Thor et al.(2020)reported the characterization of an Arabidopsis Ca2+-permeable channel,OSCA1.3,which is regulated by the BIK1 kinase to participate in this adaptive response(Figure 1).
