Mangroves are environments that have fast cycles associated with high concentration of bacterial decomposers.These are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance ...Mangroves are environments that have fast cycles associated with high concentration of bacterial decomposers.These are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance genes.This study aims to evaluate the metabolic profile of the microbiota in mangrove sediments,and verify the presence of Gram-negative bacteria resistant to meropenem.The samples were obtained from location along the Anil river and were seeded in medium supplemented with the antibiotic meropenem in increasing concentrations.The DNA was analyzed by multiplex PCR for detecting resistance genes forβ-lactam antibiotics.The bacteria were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry(MALDI-TOF MS).The genes were sequenced by ABI PRISM 3100,analyzed by MEGA 6.0 program,and the sequence identified was assessed by GenBank using the BLAST algorithm.Ecoplate■the kit was used to determine the metabolic profile of the microbiota.The results of the six bacterial isolates showed the blaKPC-2 gene and were identified as Stenotrophomonas maltophilia and Pseudomonas putida.The samples showed a greater diversity Shannon index,a rich substrate consumption and high equity.There was a metabolic richness such as carbon consumption profiles,being a factor of adaptation of pathogenic bacteria carrying resistance genes toβ-lactamics antibiotics.展开更多
Punica granatum L.possesses known antimicrobial and anti-inflammatory actions.Preparations of different parts of this plant have been commonly used in the folk medicine to prevent and treat infections;and studies have...Punica granatum L.possesses known antimicrobial and anti-inflammatory actions.Preparations of different parts of this plant have been commonly used in the folk medicine to prevent and treat infections;and studies have supported the use of P.granatum extracts for this purpose.However,little is known of the effects of P.granatum-derived fractions in severe infectious diseases such as sepsis.Here,we investigated the actions of the hexane fraction(HF),rich in lipophilic compounds,obtained from the leaves of this plant,in a murine model of polymicrobial sepsis.Oral pre-treatment with HF increased mortality in septic mice.The same animals presented with lower levels of interleukin-6,nitric oxide and hydrogen peroxide in their peritoneal lavage samples than vehicle mice.HF also increased polymorphonuclear cell accumulation in to the peritoneum of mice with sepsis.Macrophage-derived nitric oxide upon lipopolysaccharide stimuli was markedly reduced by incubation with HF.Diterpenes,triterpenes,phytosterols,vitamin E and ascorbyl palmitate were detected in the HF.These results indicate that P.granatum leaf lipophilic fractions may worsen sepsis outcome.This effect may be associated with its bioactive compounds which act synergistically or not,increasing sepsis mortality in vivo.展开更多
文摘Mangroves are environments that have fast cycles associated with high concentration of bacterial decomposers.These are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance genes.This study aims to evaluate the metabolic profile of the microbiota in mangrove sediments,and verify the presence of Gram-negative bacteria resistant to meropenem.The samples were obtained from location along the Anil river and were seeded in medium supplemented with the antibiotic meropenem in increasing concentrations.The DNA was analyzed by multiplex PCR for detecting resistance genes forβ-lactam antibiotics.The bacteria were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry(MALDI-TOF MS).The genes were sequenced by ABI PRISM 3100,analyzed by MEGA 6.0 program,and the sequence identified was assessed by GenBank using the BLAST algorithm.Ecoplate■the kit was used to determine the metabolic profile of the microbiota.The results of the six bacterial isolates showed the blaKPC-2 gene and were identified as Stenotrophomonas maltophilia and Pseudomonas putida.The samples showed a greater diversity Shannon index,a rich substrate consumption and high equity.There was a metabolic richness such as carbon consumption profiles,being a factor of adaptation of pathogenic bacteria carrying resistance genes toβ-lactamics antibiotics.
文摘Punica granatum L.possesses known antimicrobial and anti-inflammatory actions.Preparations of different parts of this plant have been commonly used in the folk medicine to prevent and treat infections;and studies have supported the use of P.granatum extracts for this purpose.However,little is known of the effects of P.granatum-derived fractions in severe infectious diseases such as sepsis.Here,we investigated the actions of the hexane fraction(HF),rich in lipophilic compounds,obtained from the leaves of this plant,in a murine model of polymicrobial sepsis.Oral pre-treatment with HF increased mortality in septic mice.The same animals presented with lower levels of interleukin-6,nitric oxide and hydrogen peroxide in their peritoneal lavage samples than vehicle mice.HF also increased polymorphonuclear cell accumulation in to the peritoneum of mice with sepsis.Macrophage-derived nitric oxide upon lipopolysaccharide stimuli was markedly reduced by incubation with HF.Diterpenes,triterpenes,phytosterols,vitamin E and ascorbyl palmitate were detected in the HF.These results indicate that P.granatum leaf lipophilic fractions may worsen sepsis outcome.This effect may be associated with its bioactive compounds which act synergistically or not,increasing sepsis mortality in vivo.
