Fructus Broussonetae extract improves cognitive function and endoplasmic reticulum stress in Alzheimer's disease models

This study investigated the effects and possible targets of Fructus Broussonetiae extract, a traditional Chinese medicinal herb, on a model of Alzheimer＇s disease induced by beta-amyloid peptide 25 35 and D-galactose. The results revealed that intragastric administration of Fructus Broussonetiae significantly increased the expression of immunoglobulin-binding protein, a key factor in the endoplasmic reticulum stress-signaling pathway in rat hippocampus. In contrast, the treatment significantly decreased expression levels of PKR-like endoplasmic reticulum kinase and C/EBP homologous protein, and substantially improved learning, memory and spatial recognition dysfunction in rats. This evidence indicates that Fructus Broussonetiae extract improves spatial learning and memory abilities in rats by affecting the regulation of hippocampal endoplasmic reticulum stress and activation of the apoptosis pathway.

安颤灵对泛素-蛋白酶体通路损伤细胞模型的保护作用

目的利用中药血清药理学方法探讨安颤灵含药血清对lactacystin诱导PCI2细胞损伤的保护作用及其可能机制。方法按照血清药理学实验方法制备安颤灵含药血清，将含药血清分为大、中、小剂量组（分别按7．3g／kg、3．65g／kg、1．83g／kg添加药物），用lactacystin建立PCI2细胞泛素-蛋白酶体系统（UPS）损伤模型。MTT方法检测各组的细胞活力。将对数生长期的PCl2细胞分为正常组（DMEM＋10％空白鼠血清）、损伤组（DMEM＋10％空白鼠血清＋10μmol／Llactacystin）、安颤灵中剂量组（含DMEM＋10％中剂量安颤灵含药血清＋10μmol／Llactacystin），通过免疫组化方法，观察各组细胞α-突触核蛋白、酪氨酸羟化酶（TH）表达的情况。结果与大、小剂量组相比，中剂量组细胞的存活率明显升高，差异有统计学意义（P〈0．05）；与正常组相比，损伤组细胞α-突触核蛋白阳性细胞数增多，而TH阳性细胞明显减少，差异有统计学意义（P〈0．05）；与损伤组相比．安颤灵中剂量组细胞α-突触核蛋白阳性细胞数减少，TH阳性细胞明显增加，差异有统计学意义（P〈0．05）。结论安颤灵含药血清能改善细胞UPS的功能，降低细胞内α-突触核蛋白的聚集并上调TH表达，从而起到提高细胞存活率，保护神经元的作用。