Pseudoalteromonas,with a ubiquitous distribution,is one of the most abundant marine bacterial genera.It is especially abundant in the deep sea and polar seas,where it has been found to have a broad metabolic capacity ...Pseudoalteromonas,with a ubiquitous distribution,is one of the most abundant marine bacterial genera.It is especially abundant in the deep sea and polar seas,where it has been found to have a broad metabolic capacity and unique co-existence strategies with other organisms.However,only a few Pseudoalteromonas phages have so far been isolated and investigated and their genomic diversity and distribution patterns are still unclear.Here,the genomes,taxonomic features and distribution patterns of Pseudoalteromonas phages are systematically analyzed,based on the microbial and viral genomes and metagenome datasets.A total of 143 complete or nearly complete Pseudoalteromonas-associated phage genomes(PSAPGs)were identifed,including 34 Pseudoalteromonas phage isolates,24 proviruses,and 85 Pseudoalteromonas-associated uncultured viral genomes(UViGs);these were assigned to 47 viral clusters at the genus level.Many integrated proviruses(n=24)and flamentous phages were detected(n=32),suggesting the prevalence of viral lysogenic life cycle in Pseudoalteromonas.PSAPGs encoded 66 types of 249 potential auxiliary metabolic genes(AMGs)relating to peptidases and nucleotide metabolism.They may also participate in marine biogeochemical cycles through the manipulation of the metabolism of their hosts,especially in the phosphorus and sulfur cycles.Siphoviral and flamentous PSAPGs were the predominant viral lineages found in polar areas,while some myoviral and siphoviral PSAPGs encoding transposase were more abundant in the deep sea.This study has expanded our understanding of the taxonomy,phylogenetic and ecological scope of marine Pseudoalteromonas phages and deepens our knowledge of viral impacts on Pseudoalteromonas.It will provide a baseline for the study of interactions between phages and Pseudoalteromonas in the ocean.展开更多
In this article the graphics relating to Figs.2 and 3 captions had been interchanged;the fgure(s)should have appeared as shown below.The original article has been corrected.Open Access This article is licensed under a...In this article the graphics relating to Figs.2 and 3 captions had been interchanged;the fgure(s)should have appeared as shown below.The original article has been corrected.Open Access This article is licensed under a Creative Commons Attribution 4.0 International License,which permits use,sharing.展开更多
Marine microbial eukaryotes are important primary producers and play critical roles in key biogeochemical cycles.Recent advances in sequencing technology have focused attention on the extent of microbial biodiversity,...Marine microbial eukaryotes are important primary producers and play critical roles in key biogeochemical cycles.Recent advances in sequencing technology have focused attention on the extent of microbial biodiversity,revealing a huge,previously underestimated phylogenetic diversity with many new lineages.This technology has now become the most important tool to understand the ecological signifcance of this huge and novel diversity in polar oceans.In particular,high-throughput sequencing technologies have been successfully applied to enumerate and compare marine microbial diversity in polar environments.Here,a brief overview of polar microbial eukaryote diversity,as revealed by in-situ surveys of the high-throughput sequencing on 18S rRNA gene,is presented.Using these‘omic’approaches,further attention still needs to be focused on diferences between specifc locations and/or entire polar oceans and on bipolar comparisons of diversity and distribution.展开更多
Microphytobenthos and sea ice algae comprise globally significant photosynthetic biofilms.While their microalgal and bacterial constituents are well characterized,there is very little information on their viral commun...Microphytobenthos and sea ice algae comprise globally significant photosynthetic biofilms.While their microalgal and bacterial constituents are well characterized,there is very little information on their viral communities or on the virus-bacteria and virus-algae interactions within them.While high levels of interaction might be expected because of the high density of cells,infection rates,particularly of microalgae,have been found to be low.It remains unclear whether this is a result of environment characteristics,developed resistance or because of the small number of studies.展开更多
基金This work was supported by the Laoshan Laboratory(No.LSKJ202203201)National Key Research and Development Program of China(2022YFC2807500)+1 种基金Natural Science Foundation of China(No.41976117,42120104006,42176111 and 42188102)and the Fundamental Research Funds for the Central Universities(202172002,201812002,202072001 and Andrew McMinn).
文摘Pseudoalteromonas,with a ubiquitous distribution,is one of the most abundant marine bacterial genera.It is especially abundant in the deep sea and polar seas,where it has been found to have a broad metabolic capacity and unique co-existence strategies with other organisms.However,only a few Pseudoalteromonas phages have so far been isolated and investigated and their genomic diversity and distribution patterns are still unclear.Here,the genomes,taxonomic features and distribution patterns of Pseudoalteromonas phages are systematically analyzed,based on the microbial and viral genomes and metagenome datasets.A total of 143 complete or nearly complete Pseudoalteromonas-associated phage genomes(PSAPGs)were identifed,including 34 Pseudoalteromonas phage isolates,24 proviruses,and 85 Pseudoalteromonas-associated uncultured viral genomes(UViGs);these were assigned to 47 viral clusters at the genus level.Many integrated proviruses(n=24)and flamentous phages were detected(n=32),suggesting the prevalence of viral lysogenic life cycle in Pseudoalteromonas.PSAPGs encoded 66 types of 249 potential auxiliary metabolic genes(AMGs)relating to peptidases and nucleotide metabolism.They may also participate in marine biogeochemical cycles through the manipulation of the metabolism of their hosts,especially in the phosphorus and sulfur cycles.Siphoviral and flamentous PSAPGs were the predominant viral lineages found in polar areas,while some myoviral and siphoviral PSAPGs encoding transposase were more abundant in the deep sea.This study has expanded our understanding of the taxonomy,phylogenetic and ecological scope of marine Pseudoalteromonas phages and deepens our knowledge of viral impacts on Pseudoalteromonas.It will provide a baseline for the study of interactions between phages and Pseudoalteromonas in the ocean.
文摘In this article the graphics relating to Figs.2 and 3 captions had been interchanged;the fgure(s)should have appeared as shown below.The original article has been corrected.Open Access This article is licensed under a Creative Commons Attribution 4.0 International License,which permits use,sharing.
基金This work is supported by the Natural Science Foundation of China(no.41676178)the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao),China(nos.2018SDKJ0104-4,2018SDKJ0406-6)+1 种基金the National Key Research and Development Program of China(no.2017YFA0603200)the Grant from Education Department of Shandong Province(S190007170001).
文摘Marine microbial eukaryotes are important primary producers and play critical roles in key biogeochemical cycles.Recent advances in sequencing technology have focused attention on the extent of microbial biodiversity,revealing a huge,previously underestimated phylogenetic diversity with many new lineages.This technology has now become the most important tool to understand the ecological signifcance of this huge and novel diversity in polar oceans.In particular,high-throughput sequencing technologies have been successfully applied to enumerate and compare marine microbial diversity in polar environments.Here,a brief overview of polar microbial eukaryote diversity,as revealed by in-situ surveys of the high-throughput sequencing on 18S rRNA gene,is presented.Using these‘omic’approaches,further attention still needs to be focused on diferences between specifc locations and/or entire polar oceans and on bipolar comparisons of diversity and distribution.
基金This study was funded by the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0406-6)National Key Research and Development Program of China(2018 YFC1406704)+1 种基金The Fundamental Research Funds for the Central Universities(201812002)the Natural Science Foundation of China(Nos.41976117 and 41606153).
文摘Microphytobenthos and sea ice algae comprise globally significant photosynthetic biofilms.While their microalgal and bacterial constituents are well characterized,there is very little information on their viral communities or on the virus-bacteria and virus-algae interactions within them.While high levels of interaction might be expected because of the high density of cells,infection rates,particularly of microalgae,have been found to be low.It remains unclear whether this is a result of environment characteristics,developed resistance or because of the small number of studies.
