Single-cell and spatial-omic technologies are providing unprecedented detail into the molecular principles underlying a wide range of developmental and disease contexts.These observations are substantially deepening m...Single-cell and spatial-omic technologies are providing unprecedented detail into the molecular principles underlying a wide range of developmental and disease contexts.These observations are substantially deepening many areas of biological research.However,despite the increasing application of these techniques to biological problems we are only revealing the tip of the iceberg,and there exist large gaps in our understanding of the behavior of single cells in vitro and in vivo that need to be filled.Additionally,the development of robust analytical tools and meta-analysis of existing data lag behind the rapid generation of new data.展开更多
Recent innovations in single cell sequencing-based technologies are shining a light on the heterogeneity of cellular populations in unprecedented detail.However,several cellular aspects are currently underutilized in ...Recent innovations in single cell sequencing-based technologies are shining a light on the heterogeneity of cellular populations in unprecedented detail.However,several cellular aspects are currently underutilized in single cell studies.One aspect is the expression and activity of transposable elements(TEs).TEs are selfish sequences of DNA that can replicate,and have been wildly successful in colonizing genomes.However,most TEs are mutated,fragmentary and incapable of transposition,yet they are actively bound by multiple transcription factors,host complex patterns of chromatin modifications,and are expressed in mRNAs as part of the transcriptome in both normal and diseased states.The contribution of TEs to development and cellular function remains unclear,and the routine inclusion of TEs in single cell sequencing analyses will potentially lead to insight into stem cells,development and human disease.展开更多
文摘Single-cell and spatial-omic technologies are providing unprecedented detail into the molecular principles underlying a wide range of developmental and disease contexts.These observations are substantially deepening many areas of biological research.However,despite the increasing application of these techniques to biological problems we are only revealing the tip of the iceberg,and there exist large gaps in our understanding of the behavior of single cells in vitro and in vivo that need to be filled.Additionally,the development of robust analytical tools and meta-analysis of existing data lag behind the rapid generation of new data.
基金This work was supported by National Natural Science Foundation of China(31970589)the Shenzhen Innovation Committee of Science and Technology grants(ZDSYS20200811144002008).
文摘Recent innovations in single cell sequencing-based technologies are shining a light on the heterogeneity of cellular populations in unprecedented detail.However,several cellular aspects are currently underutilized in single cell studies.One aspect is the expression and activity of transposable elements(TEs).TEs are selfish sequences of DNA that can replicate,and have been wildly successful in colonizing genomes.However,most TEs are mutated,fragmentary and incapable of transposition,yet they are actively bound by multiple transcription factors,host complex patterns of chromatin modifications,and are expressed in mRNAs as part of the transcriptome in both normal and diseased states.The contribution of TEs to development and cellular function remains unclear,and the routine inclusion of TEs in single cell sequencing analyses will potentially lead to insight into stem cells,development and human disease.
