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Intrauterine Exposure to Chronic 22 kHz Sound Affects Inhibitory Avoidance and Serotonergic Parameters in Forebrain Areas of Dams and Rat Offspring 被引量:1
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作者 Patrícia da Silva Oliveira Ariomester Nascimento Daniel +3 位作者 Polliana Toledo Nunes Karina Abdo Costa Hani Camille Yehia angela maria ribeiro 《Journal of Behavioral and Brain Science》 2015年第2期25-39,共15页
In the present study we evaluated the effects of chronic exposure to sounds at 22 kHz during pregnancy on the central serotonergic and behavioral parameters in Wistar rat dams after the suckling period and on their ma... In the present study we evaluated the effects of chronic exposure to sounds at 22 kHz during pregnancy on the central serotonergic and behavioral parameters in Wistar rat dams after the suckling period and on their male rat offspring. In addition, we also assessed the effects of an acute 22 kHz sound, associated with the chronic intrauterine exposure, on the emotional responses of adult offspring. The primary hypothesis was that experiencing 22 kHz stimuli during an early stage of development would interfere with brain serotonergic parameters and, later, with the adult rat’s defensive responses. The corollary question was whether a 22 kHz sound exposure would differentially affect inhibitory avoidance and escape responses and central serotonergic parameters. Female rats were divided into four groups: non-pregnant control;non-pregnant chronic exposure;pregnant control;and pregnant chronic exposure. Male offspring were divided into four groups: chronic intrauterine sound exposure;acute sound exposure in adulthood;chronic intrauterine exposure with acute exposure in adulthood;and no exposure. Chronic sound exposure affected inhibitory avoidance and serotonergic parameters in female rats. For offspring, there was an interaction between chronic and acute sound exposure effects on inhibitory avoidance response but not on escape response. There were significant effects of chronic intrauterine exposure on serotonin turnover in the hippocampus and PFC of females. For offspring, the turnover was increased by chronic exposure only in PFC, and in amygdala it was increased by acute exposure. These results illuminate the potential of an early acoustic sound exposure for causing central serotonergic and emotional behavioral changes that can persist into later periods of life. 展开更多
关键词 INTRAUTERINE 22 KHZ SOUND Exposure Defensive Responses SEROTONERGIC PARAMETERS Adult OFFSPRING
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Validation of a HPLC Method for Quantification of Thiamine and Its Phosphate Esters in Rat Brain Tissue
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作者 Polliana Toledo Nunes Patrí +2 位作者 cia da Silva Oliveira Vany Ferraz angela maria ribeiro 《Journal of Behavioral and Brain Science》 2017年第2期79-93,共15页
The present data show a fast and efficient biological sample processing method for the extraction of thiamine (vitamin B1) and its mono-(TMP) and di-(TDP) phosphate esters from hippocampus, thalamus and prefrontal cor... The present data show a fast and efficient biological sample processing method for the extraction of thiamine (vitamin B1) and its mono-(TMP) and di-(TDP) phosphate esters from hippocampus, thalamus and prefrontal cortex (PFC) and blood sample of the rodents. In addition, using the hippocampus and standards of these three compounds we validated an isocratic fluorescence HPLC procedure for a simultaneous detection of them in a single chromatogram within a total run time of about 12 min. Reproducibility for TDP, TMP and B1 was 2.66%, 4.50% and 7.43% (intraday) and 37.54%, 25.39% and 25.87% (interday), respectively. Recovery assays were between 96.0% and 101.7%. The calibration curves were linear and the concentrations of the three compounds, all in nanomolar range, were determined in the brain areas and in the blood samples. When compared to the current methods in the literature, this new method provides information on essential variables, such as linearity range and limit of detection, reproducibility and stability of thiamine, TMP and TDP in rat brain samples. The present data on sample processing and B1 and its phosphate ester level determinations are the first to be validated using hippocampus samples of rats. 展开更多
关键词 THIAMINE HPLC Rat BRAIN SAMPLES VALIDATION Method
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