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Enzyme Biosensor for Detection of Organophosphate Pesticide Residues Base on <i>Screen Printed Carbon Electrode</i>(SPCE)-Bovine Serum Albumin (BSA) 被引量:1
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作者 ani mulyasuryani Mochammad Dofir 《Engineering(科研)》 2014年第5期230-235,共6页
The maximum level of organophosphate pesticide residues in rice is 0.1 mg/kg and 0.5 mg/kg in vegetables. The control of pesticide residues in agricultural products required a method of analysis quickly and accurately... The maximum level of organophosphate pesticide residues in rice is 0.1 mg/kg and 0.5 mg/kg in vegetables. The control of pesticide residues in agricultural products required a method of analysis quickly and accurately. The research developed a biosensor for the detection of organophosphate pesticide residues in agricultural products. The research studied immobilized organophosphate hydrolase (OPH) mass and characterization of biosensor. The solution conductivity measurement in the conductivity cell consists of a 1 × 5 mm2 pair of electrodes screen printed carbon electrode (SPCE). The instrument is a converted local conductometer. From the results of study concluded that the optimum performance of the biosensor was obtained from the 105 μg OPH, at pH 8.5 with a response time of 45 seconds. In that condition the sensitivity of biosensor is 28.04 μS/ppm and 0.18 ppm detection limit and the maximum concentration of pesticide which can be measured is 1 ppm. Biosensors have been applied to measure pesticide residues in some vegetable samples. 展开更多
关键词 CONDUCTOMETRIC Biosensor ORGANOPHOSPHATE Pesticides ORGANOPHOSPHATE Hydrolase SCREEN PRINTED Carbon Electrode
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